Role of miR-2392 in driving SARS-CoV-2 infection

MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation that have a major impact on many diseases and provide an exciting avenue toward antiviral therapeutics. From patient transcriptomic data, we determined that a circulating miRNA, miR-2392, is directly involved with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) machinery during host infection. Specifically, we show that miR-2392 is key in driving downstream suppression of mitochondrial gene expression, increasing inflammation, glycolysis, and hypoxia, as well as promoting many symptoms associated with coronavirus disease 2019 (COVID-19) infection. We demonstrate that miR-2392 is present in the blood and urine of patients positive for COVID-19 but is not present in patients negative for COVID-19. These findings indicate the potential for developing a minimally invasive COVID-19 detection method. Lastly, using in vitro human and in vivo hamster models, we design a miRNA-based antiviral therapeutic that targets miR-2392, significantly reduces SARS-CoV-2 viability in hamsters, and may potentially inhibit a COVID-19 disease state in humans

Effects of increased deposition of atmospheric nitrogen on an upland Calluna moor: N and P transformations

This study determined the effects of increased N deposition on rates of N and P transformations in an upland moor. The litter layer and the surface of the organic Oh horizon were taken from plots that had received long-term additions of ammonium nitrate at rates of 40, 80 and 120 kg N ha 1 yr 1. Net mineralisation processes were measured in both field and laboratory incubations. Soil phosphomonoesterase (PME) activity and rates of N2O release were measured in laboratory incubations and root-surface PME activity measured in laboratory microcosms using Calluna vulgaris bioassay seedlings. Net mineralisation rates were relatively slow, with net ammonification consistently stimulated by N addition. Net nitrification was marginally stimulated by N addition in the laboratory incubation. N additions also increased soil and root-surface (PME) activity and rates of N2O release. Linear correlations were found between litter C:N ratio and all the above processes except net nitrification in field incubations. When compared with data from a survey of European forest sites, values of litter C:N ratio were greater than a threshold below which substantial, N input-related increases in net nitrification rates occurred. The maintenance of high C:N ratios with negligible rates of net nitrification was associated with the common presence of ericaceous litter and a mor humus layer in both this moorland as well as the forest sites. ©2005 Elsevier Ltd. All rights reserved

Effects of increased deposition of atmospheric nitrogen on an upland moor: Nitrogen budgets and nutrient accumulation

This study was designed to investigate the effect of long-term (11 years) ammonium nitrate additions on standing mass, nutrient content (% and kg ha−1), and the proportion of the added N retained within the different compartments of the system. The results showed that more than 90% of all N in the system was found in the soil, particularly in the organic (Oh) horizon. Added N increased the standing mass of vegetation and litter and the N content (kg N ha−1) of almost all measured plant, litter and soil compartments. Green tissue P and K content (kg ha−1) were increased, and N:P ratios were increased to levels indicative of P limitation. At the lowest treatment, most of the additional N was found in plant/litter compartments, but at higher treatments, there were steep increases in the amount of additional N in the underlying organic and mineral (Eag) horizons. The budget revealed that the proportion of added N found in the system as a whole increased from 60%, 80% and up to 90% in response to the 40, 80 and 120 kg N ha−1 year−1 treatments, respectively. Additions of 40 kg N ha−1 over 11 years accumulated mainly in plant and litter compartments; higher additions accumulated mainly in the organic and mineral horizons

The effect of powdery mildew infection of grape berries on juice and wine composition and on sensory properties of Chardonnay wines

The composition of juice and wine made from Chardonnay, Shiraz and Cabernet Sauvignon grapes with increasing levels of powdery mildew infection was assessed over two vintages (2000 and 2001). Chardonnay juice and wine made from grape lots with 0 %, 1-5 %, 10-30 % and 31-100 % of bunches visually infected with powdery mildew showed an increase in titratable acidity, total phenolics, hydroxycinnamates and flavonoids with increasing level of infection. In vintage 2001, Chardonnay bunches with greater than 30% powdery mildew infection had lower total soluble solids (TSS) than lower infection categories and healthy grapes. Powdery mildew infection of Cabernet Sauvignon bunches (1-20%) also resulted in lower TSS, and in lower wine phenolic concentration and spectral colour values compared with healthy grapes. The total microbial population was higher on powdery mildew affected Chardonnay, Shiraz and Cabernet Sauvignon grapes than on healthy grapes. Sensory descriptive analysis of Chardonnay wines from the 2001 vintage showed that wines made from grapes with powdery mildew infection were rated higher in 'oily' and 'viscosity' attributes than wines made from disease-free grapes. Other sensory attribute differences were also apparent, but these were likely to be related to differences in fermentation rate or secondary compositional effects of the powdery mildew infection, notably differing TSS and acidity in the juice. No mouldy or 'off aromas' were associated with wine made from infected grapes. Nevertheless, the study indicated that even very low levels of infection appear to be detrimental to wine sensory characteristics.Belinda E. Stummer, I. Leigh Francis, Andrew J. Markides and Eileen S. Scot