Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus

The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide. Such signal peptides are commonly used to direct archaeal flagellin subunits or bacterial (pseudo)pilins into extracellular macromolecular surface appendages. Likewise, S. solfataricus binding proteins have been suggested to assemble in higher ordered surface structures as well, tentatively termed the bindosome. Here we show that S. solfataricus contains a specific system that is needed for the functional surface localization of sugar binding proteins. This system, encoded by the bas (bindosome assembly system) operon, is composed of five proteins: basABC, three homologues of so-called bacterial (pseudo)pilins; BasE, a cytoplasmic ATPase; and BasF, an integral membrane protein. Deletion of either the three (pseudo)pilin genes or the basEF genes resulted in a severe defect of the cells to grow on substrates which are transported by sugar binding proteins containing class III signal peptides, while growth on glucose and maltose was restored when the corresponding genes were reintroduced in these cells. Concomitantly, ΔbasABC and ΔbasEF cells were severely impaired in glucose uptake even though the sugar binding proteins were normally secreted across the cytoplasmic membrane. These data underline the hypothesis that the bas operon is involved in the functional localization of sugar binding proteins at the cell surface of S. solfataricus. In contrast to surface structure assembly systems of Gram-negative bacteria, the bas operon seems to resemble an ancestral simplified form of these machineries.

Acute inhibition of MEK suppresses congenital melanocytic nevus syndrome in a murine model driven by activated NRAS and Wnt signaling

Congenital melanocytic nevus (CMN) syndrome is the association of pigmented melanocytic nevi with extra-cutaneous features, classically melanotic cells within the central nervous system, most frequently caused by a mutation of NRAS codon 61. This condition is currently untreatable and carries a significant risk of melanoma within the skin, brain, or leptomeninges. We have previously proposed a key role for Wnt signaling in the formation of melanocytic nevi, suggesting that activated Wnt signaling may be synergistic with activated NRAS in the pathogenesis of CMN syndrome. Some familial pre-disposition suggests a germ-line contribution to CMN syndrome, as does variability of neurological phenotypes in individuals with similar cutaneous phenotypes. Accordingly, we performed exome sequencing of germ-line DNA from patients with CMN to reveal rare or undescribed Wnt-signaling alterations. A murine model harboring activated NRASQ61K and Wnt signaling in melanocytes exhibited striking features of CMN syndrome, in particular neurological involvement. In the first model of treatment for this condition, these congenital, and previously assumed permanent, features were profoundly suppressed by acute post-natal treatment with a MEK inhibitor. These data suggest that activated NRAS and aberrant Wnt signaling conspire to drive CMN syndrome. Post-natal MEK inhibition is a potential candidate therapy for patients with this debilitating condition

Lognormal Properties of SGR 1806-20 and Implications for Other SGR Sources

The time interval between successive bursts from SGR 1806-20 and the intensity of these bursts are both consistent with lognormal distributions. Monte Carlo simulations of lognormal burst models with a range of distribution parameters have been investigated. The main conclusions are that while most sources like SGR 1806-20 should be detected in a time interval of 25 years, sources with means about 100 times longer have a probability of about 5\% of being detected in the same interval. A new breed of experiments that operate for long periods are required to search for sources with mean recurrence intervals much longer than SGR 1806-20.Comment: 4 pages, latex with seperate file containing 2 uuencoded, gzip'ed, tarred, .eps figures. Replaced with file that does not use kluwer.sty to allow automatic postscript generation. To appear in proceedings of ESLAB 2

Electronic polarization in pentacene crystals and thin films

Electronic polarization is evaluated in pentacene crystals and in thin films on a metallic substrate using a self-consistent method for computing charge redistribution in non-overlapping molecules. The optical dielectric constant and its principal axes are reported for a neutral crystal. The polarization energies P+ and P- of a cation and anion at infinite separation are found for both molecules in the crystal's unit cell in the bulk, at the surface, and at the organic-metal interface of a film of N molecular layers. We find that a single pentacene layer with herring-bone packing provides a screening environment approaching the bulk. The polarization contribution to the transport gap P=(P+)+(P-), which is 2.01 eV in the bulk, decreases and increases by only ~ 10% at surfaces and interfaces, respectively. We also compute the polarization energy of charge-transfer (CT) states with fixed separation between anion and cation, and compare to electroabsorption data and to submolecular calculations. Electronic polarization of ~ 1 eV per charge has a major role for transport in organic molecular systems with limited overlap.Comment: 10 revtex pages, 6 PS figures embedde

X-Ray Scattering Measurements of the Transient Structure of a Driven Charge-Density-Wave

We report time-resolved x-ray scattering measurements of the transient structural response of the sliding {\bf Q}$_{1}$ charge-density-wave (CDW) in NbSe$_{3}$ to a reversal of the driving electric field. The observed time scale characterizing this response at 70K varies from $\sim$ 15 msec for driving fields near threshold to $\sim$ 2 msec for fields well above threshold. The position and time-dependent strain of the CDW is analyzed in terms of a phenomenological equation of motion for the phase of the CDW order parameter. The value of the damping constant, $\gamma = (3.2 \pm 0.7) \times 10^{-19}$ eV $\cdot$ seconds $\cdot$ \AA$^{-3}$, is in excellent agreement with the value determined from transport measurements. As the driving field approaches threshold from above, the line shape becomes bimodal, suggesting that the CDW does not depin throughout the entire sample at one well-defined voltage.Comment: revtex 3.0, 7 figure

On the symmetry of minimizers

For a large class of variational problems we prove that minimizers are symmetric whenever they are $C^1$.Comment: 17 pages, to appear in Arch. Rational Mech. Anal.; added Example 7 and some reference

Influence of thermal fluctuations on quantum phase transitions in one-dimensional disordered systems: Charge density waves and Luttinger liquids

The low temperature phase diagram of 1D weakly disordered quantum systems like charge or spin density waves and Luttinger liquids is studied by a \emph{full finite temperature} renormalization group (RG) calculation. For vanishing quantum fluctuations this approach is amended by an \emph{exact} solution in the case of strong disorder and by a mapping onto the \emph{Burgers equation with noise} in the case of weak disorder, respectively. At \emph{zero} temperature we reproduce the quantum phase transition between a pinned (localized) and an unpinned (delocalized) phase for weak and strong quantum fluctuations, respectively, as found previously by Fukuyama or Giamarchi and Schulz. At \emph{finite} temperatures the localization transition is suppressed: the random potential is wiped out by thermal fluctuations on length scales larger than the thermal de Broglie wave length of the phason excitations. The existence of a zero temperature transition is reflected in a rich cross-over phase diagram of the correlation functions. In particular we find four different scaling regions: a \emph{classical disordered}, a \emph{quantum disordered}, a \emph{quantum critical} and a \emph{thermal} region. The results can be transferred directly to the discussion of the influence of disorder in superfluids. Finally we extend the RG calculation to the treatment of a commensurate lattice potential. Applications to related systems are discussed as well.Comment: 19 pages, 7 figure

Scintillation Counters for the D0 Muon Upgrade

We present the results of an upgrade to the D0 muon system. Scintillating counters have been added to the existing central D0 muon system to provide rejection for cosmic ray muons and out-of-time background, and to provide additional fast timing information for muons in an upgraded Tevatron. Performance and results from the 1994-1996 Tevatron run are presented.Comment: 30 pages, 25 postscript figure

L-band integral field spectroscopy of the HR 8799 planetary system

Stars and planetary system