Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain

Cortical interneurons, generated predominantly in the medial ganglionic eminence, migrate around and avoid the developing striatum in the subpallium en route to the cortex. This is attributable to the chemorepulsive cues of class 3 semaphorins expressed in the striatal mantle and acting through neuropilin (Nrp1 and Nrp2) receptors expressed in these cells. Cortical interneurons also express Robo receptors, and we show here that in mice lacking Robo1, but not Robo2, these cells migrate aberrantly through the striatum. In vitro experiments demonstrated that interneurons lacking Robo1 function are significantly less responsive to the effects of semaphorins. Failure to respond to semaphorin appears to be attributable to a reduction in Nrp1 and PlexinA1 receptors within these cells. Biochemical studies further demonstrated that Robo1 binds directly to Nrp1, but not to semaphorins, and this interaction is mediated by a region contained within its first two Ig domains. Thus, we show for the first time that Robo1 interacts with Nrp1 to modulate semaphorin signaling in the developing forebrain and direct the migration of interneurons through the subpallium and into the cortex

Do Femtonewton Forces Affect Genetic Function? A Review

Protein-Mediated DNA looping is intricately related to gene expression. Therefore any mechanical constraint that disrupts loop formation can play a significant role in gene regulation. Polymer physics models predict that less than a piconewton of force may be sufficient to prevent the formation of DNA loops. Thus, it appears that tension can act as a molecular switch that controls the much larger forces associated with the processive motion of RNA polymerase. Since RNAP can exert forces over 20 pN before it stalls, a ‘substrate tension switch’ could offer a force advantage of two orders of magnitude. Evidence for such a mechanism is seen in recent in vitro micromanipulation experiments. In this article we provide new perspective on existing theory and experimental data on DNA looping in vitro and in vivo . We elaborate on the connection between tension and a variety of other intracellular mechanical constraints including sequence specific curvature and supercoiling. In the process, we emphasize that the richness and versatility of DNA mechanics opens up a whole new paradigm of gene regulation to explore.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41816/1/10867_2005_Article_9002.pd

TESS discovery of a super-earth and three sub-neptunes hosted by the bright, sunlike star HD 108236

We report the discovery and validation of four extrasolar planets hosted by the nearby, bright, Sun-like (G3V) star HD 108236 using data from the Transiting Exoplanet Survey Satellite (TESS). We present transit photometry, reconnaissance, and precise Doppler spectroscopy, as well as high-resolution imaging, to validate the planetary nature of the objects transiting HD 108236, also known as the TESS Object of Interest (TOI) 1233. The innermost planet is a possibly rocky super-Earth with a period of 3.79523+0.00047-0.00044 days and has a radius of 1.586 ± 0.098 R⊗.The outer planets are sub-Neptunes, with potential gaseous envelopes, having radii of 2.068+0.10-0.091 R⊗, 2.72 ± 0.11 R⊗, and 3.12+0.13-0.12 R⊗ and periods of 6.20370+0.00064-0.00052 days, 14.17555+0.00099-0.0011 days, and 19.5917+0.0022-0.0020 days, respectively. With V and Ks magnitudes of 9.2 and 7.6, respectively, the bright host star makes the transiting planets favorable targets for mass measurements and, potentially, for atmospheric characterization via transmission spectroscopy. HD 108236 is the brightest Sun-like star in the visual (V ) band known to host four or more transiting exoplanets. The discovered planets span a broad range of planetary radii and equilibrium temperatures and share a common history of insolation from a Sun-like star (R∗ = 0.888 ± 0.017 R⊙, Teff = 5730 ± 50 K), making HD 108236 an exciting, opportune cosmic laboratory for testing models of planet formation and evolution

A hot terrestrial planet orbiting the bright M dwarf L 168-9 unveiled by TESS

We report the detection of a transiting super-Earth-sized planet (R = 1.39 ± 0.09 R⊕ ) in a 1.4-day orbit around L 168-9 (TOI-134), a bright M1V dwarf (V = 11, K = 7.1) located at 25.15 ± 0.02 pc. The host star was observed in the first sector of the Transiting Exoplanet Survey Satellite (TESS) mission. For confirmation and planet mass measurement purposes, this was followed up with ground-based photometry, seeing-limited and high-resolution imaging, and precise radial velocity (PRV) observations using the HARPS and Magellan/PFS spectrographs. By combining the TESS data and PRV observations, we find the mass of L 168-9 b to be 4.60 ± 0.56 M⊕ and thus the bulk density to be 1.74-0.33+0.44 times higher than that of the Earth. The orbital eccentricity is smaller than 0.21 (95% confidence). This planet is a level one candidate for the TESS mission's scientific objective of measuring the masses of 50 small planets, and it is one of the most observationally accessible terrestrial planets for future atmospheric characterization

A pair of tess planets spanning the radius valley around the nearby mid-m dwarf ltt 3780

We present the confirmation of two new planets transiting the nearby mid-M dwarf LTT 3780 (TIC 36724087, TOI-732, V = 13.07, K s = 8.204, R s = 0.374 R o˙, M s = 0.401 M o˙, d = 22 pc). The two planet candidates are identified in a single Transiting Exoplanet Survey Satellite sector and validated with reconnaissance spectroscopy, ground-based photometric follow-up, and high-resolution imaging. With measured orbital periods of P b = 0.77, P c = 12.25 days and sizes r p,b = 1.33 ± 0.07, r p,c = 2.30 ± 0.16 R ⊕, the two planets span the radius valley in period-radius space around low-mass stars, thus making the system a laboratory to test competing theories of the emergence of the radius valley in that stellar mass regime. By combining 63 precise radial velocity measurements from the High Accuracy Radial velocity Planet Searcher (HARPS) and HARPS-N, we measure planet masses of mpb 2.62+ 0.48 and-0.46= mpc 8.6+1.6-1.3 M⊕, which indicates that LTT 3780b has a bulk composition consistent with being Earth-like, while LTT 3780c likely hosts an extended H/He envelope. We show that the recovered planetary masses are consistent with predictions from both photoevaporation and core-powered mass-loss models. The brightness and small size of LTT 3780, along with the measured planetary parameters, render LTT 3780b and c as accessible targets for atmospheric characterization of planets within the same planetary system and spanning the radius valley

Symposium: Odor-based social behavior in mammals: signals, brain and behavior

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Hydrological mass balance of boreal watersheds in the Canadian Shield

International audienceThis study aims at evaluating the hydrological balance of large watersheds of the Canadian Shield in the James Bay area in Northwestern Quebec, Canada. The focus is set on six rivers of the Canadian Shield altogether draining more than 185,000 km2 of the Boreal Shield, Taiga Shield and Hudson Plains ecozones of Canada. River discharge measurements, geochemical data (δ2H, δ18O and electrical conductivity [EC] of water), remote sensing, and GIS models are used jointly to calculate water balances. The approach allows for partitioning the influence of rainwater, snowmelt, surface runoff, evaporation, transpiration, and groundwater discharge to the hydrological balances of watersheds. On an annual basis, the results suggest that runoff from rainwater (30–61 % of total precipitation) and snowmelt (18–40 % of total precipitation) are the main contributions to river discharge, while the contribution of groundwater discharge to rivers represents 3 of water. The stable isotope mass balances allow for estimating watershed-scale evaporation over inflow ratios ranging between 2 and 10 % and suggest that transpiration has an isotopic composition close to summer rainwater. The hydrological balances further suggest that the total pool of water stored in the active portion of watersheds represents 10–20 % of the total annual precipitation, while the exports of groundwater beyond the limits of surface watersheds are negligible. The seasonal trends in the hydrological balances of monitored watersheds were further documented to provide insights into the sensitivity of watersheds as they face climate change. The observations are used to propose recommendations for monitoring of rivers in the Canadian Shield and to identify future research needs

A compensative model for the angle-dependence of motion estimates in non-invasive vascular elastography

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