Studies of glutathione metabolism in sheep erythrocytes

The tripeptide, reduced glutathione (GSH), is present in high concentration in mammalian erythrocytes, where its major role is the protection of the cell against oxidative damage. Sheep exhibit two distinct types of erythrocyte GSH deficiency. In Finnish Landrace sheep (Finns) low GSH is inherited as an autosomal recessive trait, and is associated with a markedly diminished erythrocyte life-span, and the presence of unusually high concentrations of some erythrocyte amino acids, notably ornithine and lysine. In contrast, in Tasmanian Merino sheep (Merinos) the low GSH characteristic is inherited in an autosomal dominant manner and both the erythrocyte life-span and amino acid concentrations are normal. This Thesis describes an investigation of the biochemical mechanisms responsible for the Finn and Merino erythrocyte GSH deficiencies. The Finn and Merino sheep investigated were maintained by the ARC Animal Breeding Research Organisation, Edinburgh. Both breeds contained substantial numbers of GSH-deficient animals. In agreement with other studies, GSH-deficient Finn erythrocytes were found to contain very high concentrations of ornithine and lysine, a phenomenon not encountered in Merinos. Sheep were allotted their GSH type on the basis of their erythrocyte total non-protein reduced thiol concentration as determined by the non-specific thiol reagent 5, 5'-dithiobis-(-2-nitrobenzoate) (DTNB). The validity of equating total DTNB reactive thiol with GSH was established by estimating GSH using novel automated versions of methods employing DTNB and alloxan as chromogens. GSH-deficient erythrocytes of both breeds were also found to have a diminished GS3G concentration so that they had a diminished total glutathione content (GSH + 2GSSG). The alloxan GSH and GSSG estimates were used to calculate the redox potential of the GSH:G3SG couple in the various cell types. In both breeds the difference in redox potential between normal and GSH-deficient cells was small. It is suggested that the diminished life-span of GSH-deficient Finn cells may not be a direct consequence of their GSH status

A Model to Estimate Sediment Yield from Field-Sized Areas: Development of Model

A tool for evaluating sediment yield from field-sized areas is needed for planning management practices to control sediment yield. We developed a reasonably simple simulation model which incorporates fundamental principles of erosion, deposition, and sediment transport mechanics. The model summarizes the state-of-the-art in erosion and sediment yield modeling with appropriate simplifications required to couple the governing equations. Limited testing showed that the procedures developed here give improved estimates over the Universal Soil Loss Equation. Specific components of the model were tested using experimental data from overland flow, erodible channel, and impoundment studies. These results suggest that the model produces reasonable estimates of erosion, sediment transport, and deposition under a variety of circumstances common to field-scale areas. Alternative management practices such as conservation tillage, terracing, and contouring can be evaluated separately or in combination to determine their influence on sediment yield. Given a particular location with specified characteristics for climate, soils, topography, and crops, the model provides a means of evaluating alternative management practices to suit a particular farming operation

ARPES Study of X-Point Band Overlaps in LaB6_6 and SmB6_6 - Contrast to SrB6_6 and EuB6_6

In contrast to our recent finding of an X-point band gap in divalent hexaborides, we report here that angle resolved photoemission spectroscopy (ARPES) data shows that the gap is absent for trivalent LaB$_6$ and is absent or nearly so for mixed valent SmB$_6$. This finding demonstrates a nontrivial evolution of the band structure from divalent to trivalent hexaborides.Comment: submitted to SCES '0

Profiling Gene Expression to Distinguish the Likely Active Diazotrophs from a Sea of Genetic Potential in Marine Sediments

Nitrogen (N) cycling microbial communities in marine sediments are extremely diverse, and it is unknown whether this diversity reflects extensive functional redundancy. Sedimentary denitrifiers remove significant amounts of N from the coastal ocean and diazotrophs are typically regarded as inconsequential. Recently, N fixation has been shown to be a potentially important source of N in estuarine and continental shelf sediments. Analysis of expressed genes for nitrite reductase (nirS) and a nitrogenase subunit (nifH) was used to identify the likely active denitrifiers and nitrogen fixers in surface sediments from different seasons in Narragansett Bay (Rhode Island, USA). The overall diversity of diazotrophs expressing nifH decreased along the estuarine gradient from the estuarine head to an offshore continental shelf site. Two groups of sequences related to anaerobic sulphur/iron reducers and sulphate reducers dominated libraries of expressed nifH genes. Quantitative polymerase chain reaction (qPCR) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) data shows the highest abundance of both groups at a mid bay site, and the highest nifH expression at the head of the estuary, regardless of season. Several potential environmental factors, including water temperature, oxygen concentration and metal contamination, may influence the abundance and nifH expression of these two bacterial groups

The EBV-encoded latent membrane proteins, LMP2A and LMP2B, limit the actions of interferon by targeting interferon receptors for degradation

Although frequently expressed in Epstein–Barr virus (EBV)-positive malignancies, the role that latent membrane protein 2A and 2B (LMP2A and LMP2B) have in the oncogenic process remains obscure. Here we show a novel function for these proteins in epithelial cells, namely, their ability to modulate signalling from type I/II interferon receptors (IFNRs). We show that LMP2A- and LMP2B-expressing epithelial cells show decreased responsiveness to interferon (IFN)α and IFNγ, as assessed by STAT1 phosphorylation, ISGF3 and GAF-mediated binding to IFN-stimulated response element and IFNγ-activated factor sequence elements and luciferase reporter activation. Transcriptional profiling highlighted the extent of this modulation, with both viral proteins impacting ‘globally’ on IFN-stimulated gene expression. Although not affecting the levels of cell-surface IFNRs, LMP2A and LMP2B accelerated the turnover of IFNRs through processes requiring endosome acidification. This function may form part of EBV's strategy to limit anti-viral responses and define a novel function for LMP2A and LMP2B in modulating signalling from receptors that participate in innate immune responses

Numerical studies of the two- and three-dimensional gauge glass at low temperature

We present results from Monte Carlo simulations of the two- and three-dimensional gauge glass at low temperature using the parallel tempering Monte Carlo method. Our results in two dimensions strongly support the transition being at T_c=0. A finite-size scaling analysis, which works well only for the larger sizes and lower temperatures, gives the stiffness exponent theta = -0.39 +/- 0.03. In three dimensions we find theta = 0.27 +/- 0.01, compatible with recent results from domain wall renormalization group studies.Comment: 7 pages, 10 figures, submitted to PR

Geological controls on radon potential in Northern Ireland

Moderate and high radon potential in Northern Ireland is associated mainly with (i) the Neoproterozoic psammites, semipelites, meta-limestones, volcanics and mafic intrusives of Counties Londonderry and Tyrone; (ii) Silurian Hawick Group greywackes and, to a much more limited extent Gala Group greywackes, in the southern sector of Counties Armagh and Down; (iii) Ordovician and Silurian acid intrusives and volcanics in eastern Counties Londonderry and Tyrone; (iv) Middle-Late Devonian conglomerates in County Tyrone; (v) Lower Carboniferous (Dinantian) limestone in the western sector of Northern Ireland, especially in County Fermanagh; (vi) Palaeogene and Late Caledonian acid intrusive rocks of the Mourne Mountains Complex, Slieve Gullion Complex and Newry Granodiorite Complex in the SE sector in County Down and County Armagh. Moderate to high radon potential is sometimes associated with glacio-fluvial sand and gravel deposits where these overlie a range of bedrocks, some of which have relatively low radon potential. In this latter case the enhanced radon potential is probably caused by the high permeability of superficial deposits. Radon potential tends to be lower when bedrocks characterised by moderate or high radon potential are overlain by relatively impermeable silt-clay alluvium, glaciolacustrine, and lacustrine deposits; peat; and glacial till and moraine. Redistribution of rock debris derived from uranium-rich bedrocks, such as the Mourne Mountains granites, through glacial, alluvial and other processes can also result in higher radon potential being associated with superficial deposits relative to underlying bedrock

Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil

Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as derived from biovar trifolii [11 restriction fragment length polymorphism (RFLP) types] and 15 clones identified as viciae (seven RFLP types). These identifications were confirmed by sequencing. There were no clones related to S. meliloti nodD. For comparison, 122 strains were isolated from nodules of white clover (Trifolium repens) growing at the field site, and 134 from nodules on trap plants of T. repens inoculated with the soil. The nodule isolates were of four nodD RFLP types, with 77% being of a single type. All four of these patterns were also found among the clones from soil DNA, and the same type was the most abundant, although it made up only 34% of the trifolii-like clones. We conclude that clover selects specific genotypes from the available soil population, and that R. leguminosarum biovar trifolii was approximately five times more abundant than biovar viciae in this pasture soil, whereas S. meliloti was rare

Nature of the Spin-glass State in the Three-dimensional Gauge Glass

We present results from simulations of the gauge glass model in three dimensions using the parallel tempering Monte Carlo technique. Critical fluctuations should not affect the data since we equilibrate down to low temperatures, for moderate sizes. Our results are qualitatively consistent with earlier work on the three and four dimensional Edwards-Anderson Ising spin glass. We find that large scale excitations cost only a finite amount of energy in the thermodynamic limit, and that those excitations have a surface whose fractal dimension is less than the space dimension, consistent with a scenario proposed by Krzakala and Martin, and Palassini and Young.Comment: 5 pages, 7 figure