279 research outputs found
Aggrecanolysis in human osteoarthritis: confocal localization and biochemical characterization of ADAMTS5–hyaluronan complexes in articular cartilages
SummaryObjectiveHuman osteoarthritis (OA) is characterized by aggrecanase-mediated depletion of cartilage aggrecan. We have examined the abundance, location and some biochemical properties of the six known aggrecanases (A disintegrin and metalloproteinase with thrombospondin-like motifs 1 (ADAMTS1) 4, 5, 8, 9 and 15) in normal and OA human cartilages.MethodsFormalin-fixed, ethylenediamine tetraacetic acid (EDTA)-decalcified sections of full-depth cartilage from human OA tibial plateaus and normal control samples were studied by confocal imaging. Probes included specific antibodies to aggrecanases and two aggrecan epitopes, as well as biotinylated hyaluronan binding protein (HABP) for hyaluronan (HA) visualization. Cartilage extracts were analyzed by Western blot for the individual proteinases and aggrecan fragments.ResultsADAMTS5 was present in association with cells throughout normal cartilage and was markedly increased in OA, particularly in clonal groups in the superficial and transitional zones, where it was predominantly co-localized with HA. Consistent with the confocal analysis, a high molecular weight complex of ADAMTS5 and HA was isolated from human OA cartilage by isotonic salt extraction and chromatography on Superose 6. The complex eluted with an apparent molecular size of about 2×106 and contained major ADAMTS5 forms of 150, 60, 40 and 30kDa. The yield of most forms on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was markedly enhanced by prior digestion of the complex with either Streptomyces hyaluronidase or chondroitinase ABC.ConclusionADAMTS5 abundance and distribution in human OA cartilages is consistent with a central role for this enzyme in destructive aggrecanolysis. HA-dependent sequestration of ADAMTS5 in the pericellular matrix may be a mechanism for regulating the activity of this proteinase in human OA cartilage
AdS/CFT for Four-Point Amplitudes involving Gravitino Exchange
In this paper we compute the tree-level four-point scattering amplitude of
two dilatini and two axion-dilaton fields in type IIB supergravity in AdS5 x
S5. A special feature of this process is that there is an "exotic" channel in
which there are no singleparticle poles. Another novelty is that this process
involves the exchange of a bulk gravitino. The amplitude is interpreted in
terms of N = 4 supersymmetric Yang-Mills theory at large 't Hooft coupling.
Properties of the Operator Product Expansion are used to analyze the various
contributions from single- and double-trace operators in the weak and strongly
coupled regimes, and to determine the anomalous dimensions of semi-short
operators. The analysis is particularly clear in the exotic channel, given the
absence of BPS states.Comment: 32 pages, 1 figure. Published Version. Minor change
Dielectric functions and collective excitations in MgB_2
The frequency- and momentum-dependent dielectric function as well as the energy loss function Im[-\protect{]} are calculated for intermetallic superconductor
by using two {\it ab initio} methods: the plane-wave pseudopotential method and
the tight-binding version of the LMTO method. We find two plasmon modes
dispersing at energies -8 eV and -22 eV. The high energy
plasmon results from a free electron like plasmon mode while the low energy
collective excitation has its origin in a peculiar character of the band
structure. Both plasmon modes demonstrate clearly anisotropic behaviour of both
the peak position and the peak width. In particular, the low energy collective
excitation has practically zero width in the direction perpendicular to boron
layers and broadens in other directions.Comment: 3 pages with 10 postscript figures. Submitted to PRB on May 14 200
Surface and Image-Potential States on the MgB_2(0001) Surfaces
We present a self-consistent pseudopotential calculation of surface and
image-potential states on for both -terminated () and
-terminated () surfaces. We find a variety of very clear surface and
subsurface states as well as resonance image-potential states n=1,2 on both
surfaces. The surface layer DOS at is increased by 55% at and by
90% at the surface compared to DOS in the corresponding bulk layers.Comment: 3 pages, 6 figure
Holographic Renormalization of Foliation Preserving Gravity and Trace Anomaly
From the holographic renormalizationg group viewpoint, while the scale
transformation plays a primary role in the duality by providing the extra
dimension, the special conformal transformation seems to only play a secondary
role. We, however, claim that the space-time diffeomorphism is crucially
related to the latter. For its demonstration, we study the holographic
renormalization group flow of a foliation preserving diffeomophic theory of
gravity (a.k.a. space-time flipped Horava gravity). We find that the dual field
theory, if any, is only scale invariant but not conformal invariant. In
particular, we show that the holographic trace anomaly in four-dimension
predicts the Ricci scalar squared term that would be incompatible with the
Wess-Zumino consistency condition if it were conformal. This illustrates how
the foliation preserving diffeomophic theory of gravity could be inconsistent
with a theorem of the dual unitary quantum field theory.Comment: 18 pages, v2: reference added, v3: comments on more recent literature
added in response to referee's reques
Angular Conditions,Relations between Breit and Light-Front Frames, and Subleading Power Corrections
We analyze the current matrix elements in the general collinear (Breit)
frames and find the relation between the ordinary (or canonical) helicity
amplitudes and the light-front helicity amplitudes. Using the conservation of
angular momentum, we derive a general angular condition which should be
satisfied by the light-front helicity amplitudes for any spin system. In
addition, we obtain the light-front parity and time-reversal relations for the
light-front helicity amplitudes. Applying these relations to the spin-1 form
factor analysis, we note that the general angular condition relating the five
helicity amplitudes is reduced to the usual angular condition relating the four
helicity amplitudes due to the light-front time-reversal condition. We make
some comments on the consequences of the angular condition for the analysis of
the high- deuteron electromagnetic form factors, and we further apply the
general angular condition to the electromagnetic transition between spin-1/2
and spin-3/2 systems and find a relation useful for the analysis of the
N- transition form factors. We also discuss the scaling law and the
subleading power corrections in the Breit and light-front frames.Comment: 24 pages,2 figure
A Field-theoretical Interpretation of the Holographic Renormalization Group
A quantum-field theoretical interpretation is given to the holographic RG
equation by relating it to a field-theoretical local RG equation which
determines how Weyl invariance is broken in a quantized field theory. Using
this approach we determine the relation between the holographic C theorem and
the C theorem in two-dimensional quantum field theory which relies on the
Zamolodchikov metric. Similarly we discuss how in four dimensions the
holographic C function is related to a conjectured field-theoretical C
function. The scheme dependence of the holographic RG due to the possible
presence of finite local counterterms is discussed in detail, as well as its
implications for the holographic C function. We also discuss issues special to
the situation when mass deformations are present. Furthermore we suggest that
the holographic RG equation may also be obtained from a bulk diffeomorphism
which reduces to a Weyl transformation on the boundary.Comment: 24 pages, LaTeX, no figures; references added, typos corrected,
paragraph added to section
Tomato: a crop species amenable to improvement by cellular and molecular methods
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato. Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato. Important developments have come from molecular approaches. Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species. Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known. Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes. Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato.
TESS hunt for young and maturing exoplanets (THYME). V. A sub-neptune transiting a young star in a newly discovered 250myr association
The detection and characterization of young planetary systems offer a direct path to study the processes that shape planet evolution. We report on the discovery of a sub-Neptune-sized planet orbiting the young star HD 110082 (TOI-1098). Transit events we initially detected during TESS Cycle 1 are validated with time-series photometry from Spitzer. High-contrast imaging and high-resolution, optical spectra are also obtained to characterize the stellar host and confirm the planetary nature of the transits. The host star is a late-F dwarf (Må = 1.2Me) with a low-mass, M dwarf binary companion (Må = 0.26Me) separated by nearly one arcminute (∼6200 au). Based on its rapid rotation and Lithium absorption, HD 110082 is young, but is not a member of any known group of young stars (despite proximity to the Octans association). To measure the age of the system, we search for coeval, phase-space neighbors and compile a sample of candidate siblings to compare with the empirical sequences of young clusters and to apply quantitative age-dating techniques. In doing so, we find that HD 110082 resides in a new young stellar association we designate MELANGE-1, with an age of 250-+7050 Myr. Jointly modeling the TESS and Spitzer light curves, we measure a planetary orbital period of 10.1827 days and radius of Rp = 3.2 ± 0.1R+. HD 110082 b’s radius falls in the largest 12% of field-age systems with similar host-star mass and orbital period. This finding supports previous studies indicating that young planets have larger radii than their field-age counterparts
Paleobiology of titanosaurs: reproduction, development, histology, pneumaticity, locomotion and neuroanatomy from the South American fossil record
Fil: García, Rodolfo A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Salgado, Leonardo. Instituto de Investigación en Paleobiología y Geología. General Roca. Río Negro; ArgentinaFil: Fernández, Mariela. Inibioma-Centro Regional Universitario Bariloche. Bariloche. Río Negro; ArgentinaFil: Cerda, Ignacio A.. Instituto de Investigación en Paleobiología y Geología. Museo Provincial Carlos Ameghino. Cipolletti; ArgentinaFil: Carabajal, Ariana Paulina. Museo Carmen Funes. Plaza Huincul. Neuquén; ArgentinaFil: Otero, Alejandro. Museo de La Plata. Universidad Nacional de La Plata; ArgentinaFil: Coria, Rodolfo A.. Instituto de Paleobiología y Geología. Universidad Nacional de Río Negro. Neuquén; ArgentinaFil: Fiorelli, Lucas E.. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica. Anillaco. La Rioja; Argentin
- …