Spectroscopic parameters for silacyclopropynylidene, SiC2_2, from extensive astronomical observations toward CW Leo (IRC +10216) with the Herschel satellite

A molecular line survey has been carried out toward the carbon-rich asymptotic giant branch star CW Leo employing the HIFI instrument on board of the Herschel satellite. Numerous features from 480 GHz to beyond 1100 GHz could be assigned unambiguously to the fairly floppy SiC$_2$ molecule. However, predictions from laboratory data exhibited large deviations from the observed frequencies even after some lower frequency data from this survey were incorporated into a fit. Therefore, we present a combined fit of all available laboratory data together with data from radio-astronomical observations.Comment: 7 pages, 1 figure, J. Mol. Spectrosc., appeared; CDMS links corrected (version 2; current version: 3; may be updated later this year

REGENERATION OF TRANSGENIC PAPAYA (Carica papaya) PLANTS

Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles carrying chimeric genes coding for NPT II, GUS, and the coat protein of a mild mutant strain HA 5-1 of papaya ringspot virus. All tissue types were cultured prior to and following particle bombardment on half-strength.MS medium supplemented with 10 mg/1-1 2,4-D, 400 mg/lrI glutamine, and 6% sucrose. Upon transfer to 2,4-D-free medium containing 150 mg/l-1 kanamycin sulfate, 12 isolates regenerated somatic embryos, and five of these produced leafy shoots six to nine months following bombardment. Tissues from 11 isolates were assayed for NPTII activity, and nine were positive. Five out of 12 isolates assayed for GUS expression were positive. Three isolates were positive for both NPTII and GUS