5 research outputs found
Additional file 1: of The NOTCH3 score: a pre-clinical CADASIL biomarker in a novel human genomic NOTCH3 transgenic mouse model with early progressive vascular NOTCH3 accumulation
Supplemental materials. (DOCX 2768 kb
Additional file 4: of Fcγ receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis
NIMPR14- and F4/80-positive cells in the infiltrate and exudate in the joints of FcγRI,II,III−/− mice and their WT controls. Representative photomicrographs of (a) NIMPR14 and (b) F4/80 staining showing neutrophils and macrophages in the infiltrate and exudate of the knee joints of FcγRI,II,III−/− mice and their WT controls at day 7 after induction of antigen-induced arthritis. Original magnification ×400 for infiltrate and ×200 and ×400 for exudate. (PDF 401 kb
Additional file 2: of FcÃŽÅ‚ receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis
Gating strategy for flow cytometric analysis. Gating strategy for flow cytometric analysis used to identify CD11bposLy6Chigh and CD11blow/negLy6Chigh osteoclast precursor populations. First, single cells were selected. For identification of CD11bposLy6Chigh monocytes, cells negative for CD90.2, CD45R/B220, CD49b, NK1.1, and Ly6G and positive for CD11b were selected (gate A). Subsequently, cells were back-gated for side scatter and forward scatter to exclude cells with high granulosity (gate B), and finally Ly6Chigh cells were selected (gate C). For identification of CD11blow/negLy6Chigh, after exclusion of CD90.2-, CD45R/B220-, CD49b-, NK1.1-, Ly6G-positive cells (gate D), cells were gated for their expression of CD11b and Ly6C (CD11Blow/negLy6Chigh) (gate E). (PDF 299 kb
Additional file 3: of Fcγ receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis
NIMPR14- and F4/80-positive cells in the infiltrate and in the exudate in the joints of FcγRI,II,III,IV−/− mice and their WT controls. Representative photomicrographs of (a) NIMPR14 and (b) F4/80 staining showing neutrophils and macrophages in the infiltrate and exudate of the knee joints of FcγRI,II,III,IV−/− mice and their WT controls at day 7 after induction of antigen-induced arthritis. Original magnification ×400 for infiltrate and ×200 and ×400 for exudate. (PDF 422 kb
Additional file 1: of Fcγ receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis
Graphical representation of bone erosion scoring method and quantification of noncartilage collagenous tissue and proteoglycan (PG) depletion. a Graphical representation of the 13 locations along the patella, femur, tibia, and cruciate ligament where bone erosion was scored. b Quantification of the noncartilage collagenous tissue (blue staining in Safranin O/Fast Green staining) in femur and tibia showed no differences between naive FcγRI,II,III,IV−/− and wild-type (WT) mice. ns Not significant. c Quantification of PG depletion showed a significant decrease at the tibiofemoral area in the joints of FcγRI,II,III,IV−/− mice as compared with their WT controls (n = 10 and 14 joints per group, respectively) at both 7 and 21 days after AIA induction. Scatterplots are shown, with horizontal and vertical lines showing mean ± SEM values. ns Not significant. * P < 0.05, ** P < 0.01 versus WT controls. (PDF 437 kb