Verifying design plans and detecting deficiencies in concrete bridge using GPR

During the construction of concrete structures such as bridges, many deficiencies may occur due to an incorrect application or changes in the original design plans and construction errors. Frequently, areas with very poorly vibrated concrete, insufficiently grouted tendon ducts and incorrectly positioned reinforcement bars appear. Thus, the detection of these construction deficiencies is essential to prevent further damage to the bridge. Subsequently, a concrete specimen was prepared aimed at simulating some of the problems that can occur during the construction. The specimen was then mapped using a GPR system to check the effectiveness of this tool to provide information about those deficiencies. The acquisition was carried out in reflection mode and the results were further processed using 3D reconstruction software in order to obtain a more realistic and comprehensible image. These measurements showed rather good results. The 3D image provided much more detailed information about the elements placed inside the specimen relatively to 2D radargrams, which are generally used for primary target identification.Fundação para a Ciência e a Tecnologia (FCT) - POCTI SFRH/BD/6409/2001.Sustainable Bridges” European project FP6-PLT-0165

Utilização do radar de prospecção geotécnica na localização das bainhas de pré-esforço nas pontes da Barra e Lanheses

Algumas pontes de betão armado, em serviço há mais de 30 anos, apresentam, actualmente, elevados índices de deterioração, que têm levado as entidades a proceder à sua beneficiação e reforço estrutural. Neste artigo, o georadar é utilizado para localizar elementos estruturais fundamentais à segurança de duas pontes de betão armado, que doutra maneira, poderiam ser danificadas e, assim, por em causa a segurança das mesmas.União Europeia (UE) - Projecto “Sustainable Bridges” - FP6-PLT-01653

Application of radar techniques to the verification of design plans and the detection of defects in concrete bridges

Non-destructive tests (NDT) are an essential tool used in special inspections to gather detailed information about the condition of a bridge. The inspection of bridge decks is a critical task, and, currently, can be successfully carried out using a wide range of NDT techniques. Nevertheless, some of these techniques are excessively expensive and time consuming. One of these techniques, the ground penetrating radar (GPR), has been used for some decades in the non-destructive inspection and diagnosis of concrete bridges. GPR is useful to find general information about the true position of reinforcement and tendon ducts, and check the quality of the construction and materials. A significant number of reinforced and prestressed concrete bridges are deteriorating at a rapid rate and need to be repaired and strengthened. During these rehabilitation processes, designers are often faced with a lack of original design plans and unawareness of the real position of reinforcement and tendon ducts. In this paper, three case studies of the use of GPR techniques for the inspection of concrete bridges are presented and analysed. The main aim of this research is to show the strong need and usefulness of these techniques, which can provide non-visible information about structural geometry and integrity required for strengthening and rehabilitation purposes.L. T. would like to acknowledge the support from the 'Sustainable Bridges' European project, grant number FP6-PLT-01653 (www.sustainablebridges.net). F. M. F. acknowledges the partial funding of this work by the FCT through the scholarship POCTI SFRH/BPD/26706/2005

Radar investigation on civil structures using 3D data reconstruction and transmission tomography

Non-destructive investigation using Ground Penetrating Radar is becoming more popular during the inspection of civil structures. Currently, traditional 2D imaging is also used as a preliminary tool to find possible areas of interest for more detailed inspection, which can be accomplished by 3D image reconstruction or tomography techniques. In this paper, a general overview of the work done at University of Minho regarding these techniques is presented. Data acquisition was performed on two masonry walls and on one large concrete specimen. In case of masonry specimens traditional 2D imaging was supplemented by the use of transmission tomography. Data acquisition on the concrete specimen was done in reflection mode and results were further processed using 3D reconstruction software. Results from these specimens are very promising. Radar tomography and 3D image reconstruction techniques provided much more detailed information about structural integrity and shapes and location of the voids placed inside the tested specimens relatively to 2D imaging originally used for potential target identification.Portuguese Foundation for the Science and Technology (FCT) - “Sustainable Bridges” European project by the grant number FP6-PLT-01653, grant number POCTI SFRH/BD/6409/200

Practical implications of GPR investigation using 3D data reconstruction and transmission tomography

Non-destructive investigation using ground penetrating radar is becoming more popular in the inspection of civil structures. Currently, traditional 2D imaging is used as a preliminary tool to fi nd possible areas of interest for more detailed inspection, which can be accomplished by more advanced techniques like 3D image reconstruction or tomography. In this paper, a general overview of the work done at University of Minho regarding these techniques is presented, together with their limitations and advantages over typical radargrams, with implications for civil engineering applications. For this purpose, data acquisition on two large masonry walls and one large concrete specimen have been carried out, using refl ection mode, 3D reconstruction and transmission tomography. The specimens have been specially built for non-destructive inspection techniques testing, incorporating different materials and internal voids. Radar tomography and 3D image reconstruction techniques provided much more detailed information about structural integrity and shapes and location of the voids, when compared to 2D imaging originally used for potential target identification.Fundação para a Ciência e a Tecnologia (FCT) - POCTI SFRH/BD/6409/2001"Sustainable Bridges" European project - FP6-PLT-0165

Critical Early Roles for col27a1a and col27a1b in Zebrafish Notochord Morphogenesis, Vertebral Mineralization and Post-embryonic Axial Growth

Fibrillar collagens are well known for their links to human diseases, with which all have been associated except for the two most recently identified fibrillar collagens, type XXIV collagen and type XXVII collagen. To assess functions and potential disease phenotypes of type XXVII collagen, we examined its roles in zebrafish embryonic and post-embryonic development.We identified two type XXVII collagen genes in zebrafish, col27a1a and col27a1b. Both col27a1a and col27a1b were expressed in notochord and cartilage in the embryo and early larva. To determine sites of type XXVII collagen function, col27a1a and col27a1b were knocked down using morpholino antisense oligonucleotides. Knockdown of col27a1a singly or in conjunction with col27a1b resulted in curvature of the notochord at early stages and formation of scoliotic curves as well as dysmorphic vertebrae at later stages. These defects were accompanied by abnormal distributions of cells and protein localization in the notochord, as visualized by transmission electron microscopy, as well as delayed vertebral mineralization as detected histologically.Together, our findings indicate a key role for type XXVII collagen in notochord morphogenesis and axial skeletogenesis and suggest a possible human disease phenotype

Catalytic C(sp3)-H bond activation in tertiary alkylamines.

The development of robust catalytic methods to assemble tertiary alkylamines provides a continual challenge to chemical synthesis. In this regard, transformation of a traditionally unreactive C-H bond, proximal to the nitrogen atom, into a versatile chemical entity would be a powerful strategy for introducing functional complexity to tertiary alkylamines. A practical and selective metal-catalysed C(sp3)-H activation facilitated by the tertiary alkylamine functionality, however, remains an unsolved problem. Here, we report a Pd(II)-catalysed protocol that appends arene feedstocks to tertiary alkylamines via C(sp3)-H functionalization. A simple ligand for Pd(II) orchestrates the C-H activation step in favour of deleterious pathways. The reaction can use both simple and complex starting materials to produce a range of multifaceted γ-aryl tertiary alkylamines and can be rendered enantioselective. The enabling features of this transformation should be attractive to practitioners of synthetic and medicinal chemistry as well as in other areas that use biologically active alkylamines

Pair-Wise Regulation of Convergence and Extension Cell Movements by Four Phosphatases via RhoA

Various signaling pathways regulate shaping of the main body axis during early vertebrate development. Here, we focused on the role of protein-tyrosine phosphatase signaling in convergence and extension cell movements. We identified Ptpn20 as a structural paralogue of PTP-BL and both phosphatases were required for normal gastrulation cell movements. Interestingly, knockdowns of PTP-BL and Ptpn20 evoked similar developmental defects as knockdown of RPTPα and PTPε. Co-knockdown of RPTPα and PTP-BL, but not Ptpn20, had synergistic effects and conversely, PTPε and Ptpn20, but not PTP-BL, cooperated, demonstrating the specificity of our approach. RPTPα and PTPε knockdowns were rescued by constitutively active RhoA, whereas PTP-BL and Ptpn20 knockdowns were rescued by dominant negative RhoA. Consistently, RPTPα and PTP-BL had opposite effects on RhoA activation, both in a PTP-dependent manner. Downstream of the PTPs, we identified NGEF and Arhgap29, regulating RhoA activation and inactivation, respectively, in convergence and extension cell movements. We propose a model in which two phosphatases activate RhoA and two phosphatases inhibit RhoA, resulting in proper cell polarization and normal convergence and extension cell movements

The Role of Glypicans in Wnt Inhibitory Factor-1 Activity and the Structural Basis of Wif1's Effects on Wnt and Hedgehog Signaling

Proper assignment of cellular fates relies on correct interpretation of Wnt and Hedgehog (Hh) signals. Members of the Wnt Inhibitory Factor-1 (WIF1) family are secreted modulators of these extracellular signaling pathways. Vertebrate WIF1 binds Wnts and inhibits their signaling, but its Drosophila melanogaster ortholog Shifted (Shf) binds Hh and extends the range of Hh activity in the developing D. melanogaster wing. Shf activity is thought to depend on reinforcing interactions between Hh and glypican HSPGs. Using zebrafish embryos and the heterologous system provided by D. melanogaster wing, we report on the contribution of glypican HSPGs to the Wnt-inhibiting activity of zebrafish Wif1 and on the protein domains responsible for the differences in Wif1 and Shf specificity. We show that Wif1 strengthens interactions between Wnt and glypicans, modulating the biphasic action of glypicans towards Wnt inhibition; conversely, glypicans and the glypican-binding “EGF-like” domains of Wif1 are required for Wif1's full Wnt-inhibiting activity. Chimeric constructs between Wif1 and Shf were used to investigate their specificities for Wnt and Hh signaling. Full Wnt inhibition required the “WIF” domain of Wif1, and the HSPG-binding EGF-like domains of either Wif1 or Shf. Full promotion of Hh signaling requires both the EGF-like domains of Shf and the WIF domains of either Wif1 or Shf. That the Wif1 WIF domain can increase the Hh promoting activity of Shf's EGF domains suggests it is capable of interacting with Hh. In fact, full-length Wif1 affected distribution and signaling of Hh in D. melanogaster, albeit weakly, suggesting a possible role for Wif1 as a modulator of vertebrate Hh signaling