9,933 research outputs found
Non-destructive imaging of an individual protein
The mode of action of proteins is to a large extent given by their ability to
adopt different conformations. This is why imaging single biomolecules at
atomic resolution is one of the ultimate goals of biophysics and structural
biology. The existing protein database has emerged from X-ray crystallography,
NMR or cryo-TEM investigations. However, these tools all require averaging over
a large number of proteins and thus over different conformations. This of
course results in the loss of structural information. Likewise it has been
shown that even the emergent X-FEL technique will not get away without
averaging over a large quantity of molecules. Here we report the first
recordings of a protein at sub-nanometer resolution obtained from one
individual ferritin by means of low-energy electron holography. One single
protein could be imaged for an extended period of time without any sign of
radiation damage. Since ferritin exhibits an iron core, the holographic
reconstructions could also be cross-validated against TEM images of the very
same molecule by imaging the iron cluster inside the molecule while the protein
shell is decomposed
Development of digital computer program for thermal network correction. Phase 2 - Program development. Phase 3 - Demonstration/application Final report
Developing digital computer program for correcting soft parameters of thermal network by Kalman filtering metho
Development of a Radioimmunoassay for Hog Pancreatic Kallikrein and its Apllication in Pharmacological Studies
Solution to the twin image problem in holography
While the invention of holography by Dennis Gabor truly constitutes an
ingenious concept, it has ever since been troubled by the so called twin image
problem limiting the information that can be obtained from a holographic
record. Due to symmetry reasons there are always two images appearing in the
reconstruction process. Thus, the reconstructed object is obscured by its
unwanted out of focus twin image. Especially for emission electron as well as
for x- and gamma-ray holography, where the source-object distances are small,
the reconstructed images of atoms are very close to their twin images from
which they can hardly be distinguished. In some particular instances only,
experimental efforts could remove the twin images. More recently, numerical
methods to diminish the effect of the twin image have been proposed but are
limited to purely absorbing objects failing to account for phase shifts caused
by the object. Here we show a universal method to reconstruct a hologram
completely free of twin images disturbance while no assumptions about the
object need to be imposed. Both, amplitude and true phase distributions are
retrieved without distortion
Electron-phonon coupling in 122 Fe pnictides analyzed by femtosecond time-resolved photoemission
Based on results from femtosecond time-resolved photoemission, we compare
three different methods for determination of the electron-phonon coupling
constant {\lambda} in Eu and Ba-based 122 FeAs compounds. We find good
agreement between all three methods, which reveal a small {\lambda} < 0.2. This
makes simple electron-phonon mediated superconductivity unlikely in these
compounds.Comment: 11 pages, 3 figure
MAGIC sensitivity to millisecond-duration optical pulses
The MAGIC telescopes are a system of two Imaging Atmospheric Cherenkov
Telescopes (IACTs) designed to observe very high energy (VHE) gamma rays above
~50 GeV. However, as IACTs are sensitive to Cherenkov light in the UV/blue and
use photo-detectors with a time response well below the ms scale, MAGIC is also
able to perform simultaneous optical observations. Through an alternative
system installed in the central PMT of MAGIC II camera, the so-called central
pixel, MAGIC is sensitive to short (1ms - 1s) optical pulses. Periodic signals
from the Crab pulsar are regularly monitored. Here we report for the first time
the experimental determination of the sensitivity of the central pixel to
isolated 1-10 ms long optical pulses. The result of this study is relevant for
searches of fast transients such as Fast Radio Bursts (FRBs).Comment: Proceedings of the 35th International Cosmic Ray Conference (ICRC
2017), Bexco, Busan, Korea (arXiv:1708.05153
X-ray absorption spectroscopy on layered cobaltates Na_xCoO_2
Measurements of polarization and temperature dependent soft x-ray absorption
have been performed on Na_xCoO_2 single crystals with x=0.4 and x=0.6. They
show a deviation of the local trigonal symmetry of the CoO_6 octahedra, which
is temperature independent in a temperature range between 25 K and 372 K. This
deviation was found to be different for Co^{3+} and Co^{4+} sites. With the
help of a cluster calculation we are able to interpret the Co L_{23}-edge
absorption spectrum and find a doping dependent energy splitting between the
t_{2g} and the e_g levels (10Dq) in Na_xCoO_2.Comment: 7 pages, 8 figure
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