External egg morphology of common stored-product pests from the families Anobiidae (Ptininae) and Dermestidae (Coleoptera)

External egg morphology of some common stored-product pests from the families Anobiidae (Ptininae: Ptinus, Niptus) and Dermestidae (Trogoderma, Reesa) based on optical and scanning electron microscope (SEM) micrographs are presented. Diagnostic characteristics are described. Differences in the eggs of these families, genera and some species were found with respect to shape, size and surface structures. Keywords: Egg morphology, SEM micrographs, Stored-product pest identificatio

The Flower-Like Hierarchical Architectures Assembled from Aniline Oligomers

The flower-like hierarchical architectures assembled from aniline oligomers by a template-free method are reported. They are important because of their close relation to a conducting polymer, polyaniline. Their formation process is ascribed to the self-assembly of oligoanilines under non-covalent interactions, such as hydrogen bonding, hydrophobic forces, and π–π stacking. The model of directional growth is offered to explain the formation of petal-like objects and, subsequently, flowers. In order to investigate the chemical structure of the oligomers, a series of characterizations have been carried out, such as UV–visible, Fourier-transform infrared and Raman spectroscopies. Based on the results of characterization methods, a formation mechanism of the aniline oligomers and their self-assembly are proposed. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3484

The Formation of Oligoaniline Microspheres in Alkaline Media

Aniline oligomers are generally believed to be responsible for the self-assembly that guides the growth of polyaniline nanostructures. The oxidations of aniline with ammonium peroxydisulfate, which are started and finished above pH 2.5, produce aniline oligomers only. Under alkaline conditions, oligoaniline microspheres spheres are formed as the dominating morphology. They will be potentially useful in applications that do not require conductivity, such as in electrorheology, corrosion protection, as ionic conductors or catalyst supports. Aniline oligomers prepared at alkaline conditions as microspheres have been studied by UV–Vis, infrared and Raman spectroscopies in the combination with optical and electron microscopic techniques. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/3483

Integrated force interaction simulation model for milling strategy optimization of thin-walled Blisk blade machining

Complex shaped thin-walled blades that are extensively used in jet engines or stream turbines are very difficult to machine due to low rigidity of the blades, typically limited space between the blades and strict requirements on the surface quality and accuracy. The paper focuses on multi-axis machining of thin-walled and complex shaped Blisk blades made of aluminium alloys. The resulting surface quality and accuracy is mainly affected by the risk of elevated vibration occurrence, both forced and self-excited, and static deflections between the compliant tool and workpiece. An innovative integration of the transformed FE model of the blade into virtual machining simulation has been proposed, allowing to effectively solve the complex optimization task considering both the criterion of stable machining condition and static deflections as well. When choosing a machining strategy and cutting conditions, there are many variables that fundamentally affect the process. These variables are not easy to choose correctly the first time, so it is advisable to choose to use a simulation model in production preparation. The proposed simulation model allowed to effectively optimize the process parameters to keep the machining process stable and the static deformation of tool and workpiece under a defined level. The proposed model and optimization strategy was validated on a thin-walled blade machining. At the top part of the blade, the surface roughness decreased from 1.6 Ra to 0.84 Ra, and the maximum deviations from the reference model were reduced from 0.18 mm to 0.08 mm

Investigating substitutions in antibody–antigen complexes using molecular dynamics: a case study with broad-spectrum, Influenza A antibodies

In studying the binding of host antibodies to the surface antigens of pathogens, the structural and functional characterization of antibody–antigen complexes by X-ray crystallography and binding assay is important. However, the characterization requires experiments that are typically time consuming and expensive: thus, many antibody–antigen complexes are under-characterized. For vaccine development and disease surveillance, it is often vital to assess the impact of amino acid substitutions on antibody binding. For example, are there antibody substitutions capable of improving binding without a loss of breadth, or antigen substitutions that lead to antigenic escape? The questions cannot be answered reliably from sequence variation alone, exhaustive substitution assays are usually impractical, and alanine scans provide at best an incomplete identification of the critical residue–residue interactions. Here, we show that, given an initial structure of an antibody bound to an antigen, molecular dynamics simulations using the energy method molecular mechanics with Generalized Born surface area (MM/GBSA) can model the impact of single amino acid substitutions on antibody–antigen binding energy. We apply the technique to three broad-spectrum antibodies to influenza A hemagglutinin and examine both previously characterized and novel variant strains observed in the human population that may give rise to antigenic escape. We find that in some cases the impact of a substitution is local, while in others it causes a reorientation of the antibody with wide-ranging impact on residue–residue interactions: this explains, in part, why the change in chemical properties of a residue can be, on its own, a poor predictor of overall change in binding energy. Our estimates are in good agreement with experimental results—indeed, they approximate the degree of agreement between different experimental techniques. Simulations were performed on commodity computer hardware; hence, this approach has the potential to be widely adopted by those undertaking infectious disease research. Novel aspects of this research include the application of MM/GBSA to investigate binding between broadly binding antibodies and a viral glycoprotein; the development of an approach for visualizing substrate–ligand interactions; and the use of experimental assay data to rescale our predictions, allowing us to make inferences about absolute, as well as relative, changes in binding energy

Prognos (R) in the diagnosis of amalgam hypersensitivity - A diagnostic case-control study

Objective: We aimed to investigate whether the Prognos (R) device might be a useful tool in the diagnosis of disorders suspected to be due to dental amalgam fillings. Participants and Methods: A diagnostic case-control study was performed in 27 patients who complained about health problems attributed to amalgam ( cases), 27 healthy volunteers with amalgam fillings ( controls I), and 27 healthy amalgam-free volunteers ( controls II). All participants were tested before and after application of 300 mg DMPS (2.3-dimercapto-1-propanesulfonic acid) with Prognos, a diagnostic device for the energetic measurement of Traditional Chinese Medicine meridians. In addition, mercury was measured in blood, urine, and saliva, and a lymphocyte transformation test (LTT) was performed. Results: Diagnoses derived from the first and second Prognos testing did not agree above chance (Cohen's Kappa = -0.11, 95% confidence interval -0.33 to 0.10; p = 0.30). Agreement for secondary outcome measures was poor, too. Prognos measurements did not differ between cases and controls. Correlations with measurements in urine, blood and saliva were low. Conclusion: In this study Prognos could not be shown to be a useful tool in the diagnosis of disorders suspected to be due to dental amalgam fillings

Advancing Scientific Discourse in the Controversy Surrounding the Comprehensive System for the Rorschach: A Rejoinder to Meyer (2000)

A recent commentary by Meyer (2000) in the Journal of Personality Assessment alleged that Rorschach critic Wood and his colleagues had intentionally published information that they knew to be in error. To substantiate this contention, Meyer’s commentary published information that was part of the peer review process at another journal. In this rejoinder, we present factual information that shows we have consistently acted in good faith. This rejoinder suggests that the scientific debate regarding the Comprehensive System for the Rorschach is unlikely to be advanced by speculating about the intentions of Rorschach critics, or by publishing information from the peer review process that is usually kept confidential