79 research outputs found
The CRESST II Dark Matter Search
Direct Dark Matter detection with cryodetectors is briefly discussed, with
particular mention of the possibility of the identification of the recoil
nucleus. Preliminary results from the CREEST II Dark Matter search, with 730
kg-days of data, are presented. Major backgrounds and methods of identifying
and dealing with them are indicated.Comment: Talk at DSU workshop, ITP Beijing, Oct. 2011. 9 figures, 2 table
Results from 730 kg days of the CRESST-II Dark Matter Search
The CRESST-II cryogenic Dark Matter search, aiming at detection of WIMPs via
elastic scattering off nuclei in CaWO crystals, completed 730 kg days of
data taking in 2011. We present the data collected with eight detector modules,
each with a two-channel readout; one for a phonon signal and the other for
coincidently produced scintillation light. The former provides a precise
measure of the energy deposited by an interaction, and the ratio of
scintillation light to deposited energy can be used to discriminate different
types of interacting particles and thus to distinguish possible signal events
from the dominant backgrounds. Sixty-seven events are found in the acceptance
region where a WIMP signal in the form of low energy nuclear recoils would be
expected. We estimate background contributions to this observation from four
sources: 1) "leakage" from the e/\gamma-band 2) "leakage" from the
\alpha-particle band 3) neutrons and 4) Pb-206 recoils from Po-210 decay. Using
a maximum likelihood analysis, we find, at a high statistical significance,
that these sources alone are not sufficient to explain the data. The addition
of a signal due to scattering of relatively light WIMPs could account for this
discrepancy, and we determine the associated WIMP parameters.Comment: 17 pages, 13 figure
Status of the CRESST Dark Matter Search
The CRESST experiment aims for a detection of dark matter in the form of
WIMPs. These particles are expected to scatter elastically off the nuclei of a
target material, thereby depositing energy on the recoiling nucleus. CRESST
uses scintillating CaWO4 crystals as such a target. The energy deposited by an
interacting particle is primarily converted to phonons which are detected by
transition edge sensors. In addition, a small fraction of the interaction
energy is emitted from the crystals in the form of scintillation light which is
measured in coincidence with the phonon signal by a separate cryogenic light
detector for each target crystal. The ratio of light to phonon energy permits
the discrimination between the nuclear recoils expected from WIMPs and events
from radioactive backgrounds which primarily lead to electron recoils. CRESST
has shown the success of this method in a commissioning run in 2007 and, since
then, further investigated possibilities for an even better suppression of
backgrounds. Here, we report on a new class of background events observed in
the course of this work. The consequences of this observation are discussed and
we present the current status of the experiment.Comment: Proceedings of the 13th International Workshop on Low Temperature
Detectors, 4 pages, 3 figure
Composite CaWO4 Detectors for the CRESST-II Experiment
CRESST-II, standing for Cryogenic Rare Events Search with Superconducting
Thermometers phase II, is an experiment searching for Dark Matter. In the LNGS
facility in Gran Sasso, Italy, a cryogenic detector setup is operated in order
to detect WIMPs by elastic scattering off nuclei, generating phononic lattice
excitations and scintillation light. The thermometers used in the experiment
consist of a tungsten thin-film structure evaporated onto the CaWO4 absorber
crystal. The process of evaporation causes a decrease in the scintillation
light output. This, together with the need of a big-scale detector production
for the upcoming EURECA experiment lead to investigations for producing
thermometers on smaller crystals which are glued onto the absorber crystal. In
our Run 31 we tested composite detectors for the first time in the Gran Sasso
setup. They seem to produce higher light yields as hoped and could provide an
additional time based discrimination mechanism for low light yield clamp
events.Comment: Proceedings of the Thirteenth International Workshop on Low
Temperature Detectors 4 pages, 9 figure
Effects of deletion of the Streptococcus pneumoniae lipoprotein diacylglyceryl transferase gene lgt on ABC transporter function and on growth in vivo
Lipoproteins are an important class of surface associated proteins that have diverse roles and frequently are involved in the virulence of bacterial pathogens. As prolipoproteins are attached to the cell membrane by a single enzyme, prolipoprotein diacylglyceryl transferase (Lgt), deletion of the corresponding gene potentially allows the characterisation of the overall importance of lipoproteins for specific bacterial functions. We have used a Δlgt mutant strain of Streptococcus pneumoniae to investigate the effects of loss of lipoprotein attachment on cation acquisition, growth in media containing specific carbon sources, and virulence in different infection models. Immunoblots of triton X-114 extracts, flow cytometry and immuno-fluorescence microscopy confirmed the Δlgt mutant had markedly reduced lipoprotein expression on the cell surface. The Δlgt mutant had reduced growth in cation depleted medium, increased sensitivity to oxidative stress, reduced zinc uptake, and reduced intracellular levels of several cations. Doubling time of the Δlgt mutant was also increased slightly when grown in medium with glucose, raffinose and maltotriose as sole carbon sources. These multiple defects in cation and sugar ABC transporter function for the Δlgt mutant were associated with only slightly delayed growth in complete medium. However the Δlgt mutant had significantly reduced growth in blood or bronchoalveolar lavage fluid and a marked impairment in virulence in mouse models of nasopharyngeal colonisation, sepsis and pneumonia. These data suggest that for S. pneumoniae loss of surface localisation of lipoproteins has widespread effects on ABC transporter functions that collectively prevent the Δlgt mutant from establishing invasive infection
Discrimination of Recoil Backgrounds in Scintillating Calorimeters
The alpha decay of \n{{}^{210}Po} is a dangerous background to rare event
searches. Here, we describe observations related to this alpha decay in the
Cryogenic Rare Event Search with Superconducting Thermometers (CRESST). We find
that lead nuclei show a scintillation light yield in our \n{CaWO_4} crystals
of relative to electrons of the same energy. We describe a
way to discriminate this source of nuclear recoil background by means of a
scintillating foil, and demonstrate its effectiveness. This leads to an
observable difference in the pulse shape of the light detector, which can be
used to tag these events. Differences in pulse shape of the phonon detector
between lead and electron recoils are also extracted, opening the window to
future additional background suppression techniques based on pulse shape
discrimination in such experiments.Comment: 5 pages, 9 figure
Electron and Gamma Background in CRESST Detectors
The CRESST experiment monitors 300g CaWO_4 crystals as targets for particle
interactions in an ultra low background environment. In this paper, we analyze
the background spectra that are recorded by three detectors over many weeks of
data taking. Understanding these spectra is mandatory if one wants to further
reduce the background level, and allows us to cross-check the calibration of
the detectors. We identify a variety of sources, such as intrinsic
contaminations due to primordial radioisotopes and cosmogenic activation of the
target material. In particular, we detect a 3.6keV X-ray line from the decay of
41-Ca with an activity of (26\pm4)\mu Bq, corresponding to a ratio
41-Ca/40-Ca=(2.2\pm0.3)\times10^{-16}.Comment: 7 pages, 12 figure
Oligomeric Coiled-Coil Adhesin YadA Is a Double-Edged Sword
Yersinia adhesin A (YadA) is an essential virulence factor for the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis. Suprisingly, it is a pseudogene in Yersinia pestis. Even more intriguing, the introduction of a functional yadA gene in Y. pestis EV76 was shown to correlate with a decrease in virulence in a mouse model. Here, we report that wild type (wt) Y. enterocolitica E40, as well as YadA-deprived E40 induced the synthesis of neutrophil extracellular traps (NETs) upon contact with neutrophils, but only YadA-expressing Y. enterocolitica adhered to NETs and were killed. As binding seemed to be a prerequisite for killing, we searched for YadA-binding substrates and detected the presence of collagen within NETs. E40 bacteria expressing V98D,N99A mutant YadA with a severely reduced ability to bind collagen were found to be more resistant to killing, suggesting that collagen binding contributes significantly to sensitivity to NETs. Wt Y. pestis EV76 were resistant to killing by NETs, while recombinant EV76 expressing YadA from either Y. pseudotuberculosis or Y. enterocolitica were sensitive to killing by NETs, outlining the importance of YadA for susceptibility to NET-dependent killing. Recombinant EV76 endowed with YadA from Y. enterocolitica were also less virulent for the mouse than wt EV76, as shown before. In addition, EV76 carrying wt YadA were less virulent for the mouse than EV76 expressing YadAV98D,N99A. The observation that YadA makes Yersinia sensitive to NETs provides an explanation as for why evolution selected for the inactivation of yadA in the flea-borne Y. pestis and clarifies an old enigma. Since YadA imposes the same cost to the food-borne Yersinia but was nevertheless conserved by evolution, this observation also illustrates the duality of some virulence functions
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