The relationship between light, dopamine release and horizontal cell coupling in the mudpuppy retina.

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/110002/1/tjp19914401291.pd

Techniques for improved convergence in neighboring optimum guidance

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/77140/1/AIAA-1969-888-643.pd

CB1 Receptor Antagonism Blocks Stress-Potentiated Reinstatement of Cocaine Seeking in Rats

Rationale Under some conditions, stress, rather than directly triggering cocaine seeking, potentiates reinstatement to other stimuli, including a subthreshold cocaine dose. The mechanisms responsible for stress-potentiated reinstatement are not well defined. Endocannabinoid signaling is increased by stress and regulates synaptic transmission in brain regions implicated in motivated behavior. Objectives The objective of this study was to test the hypothesis that cannabinoid type 1 receptor (CB1R) signaling is required for stress-potentiated reinstatement of cocaine seeking in rats. Methods Following i.v. cocaine self-administration (2 h access/day) and extinction in male rats, footshock stress alone does not reinstate cocaine seeking but reinstatement is observed when footshock is followed by an injection of an otherwise subthreshold dose of cocaine (2.5 mg/kg, i.p.). CB1R involvement was tested by systemic administration of the CB1R antagonist AM251 (0, 1, or 3 mg/kg, i.p.) prior to testing for stress-potentiated reinstatement. Results Stress-potentiated reinstatement was blocked by both 1 and 3 mg/kg AM251. By contrast, AM251 only attenuated food-reinforced lever pressing at the higher dose (i.e., 3 mg/kg) and did not affect locomotor activity at either dose tested. Neither high-dose cocaine-primed reinstatement (10 mg/kg, i.p.) nor footshock stress-triggered reinstatement following long-access cocaine self-administration (6 h access/day) was affected by AM251 pretreatment. Footshock stress increased concentrations of both endocannabinoids, N-arachidonylethanolamine and 2-arachidonoylglycerol, in regions of the prefrontal cortex. Conclusions These findings demonstrate that footshock stress increases prefrontal cortical endocannabinoids and stress-potentiated reinstatement is CB1R-dependent, suggesting that CB1R is a potential therapeutic target for relapse prevention, particularly in individuals whose cocaine use is stress-related

Detection Of Brugia Malayi And Brugia Pahangi Parasites By Biotinlabeled Dna Probes

Morphologically, the larvae of Brugian parasites are difficult to differentiate by conventional methods. Recently, radioactive labeled DNA probes2'3 have been developed to distinguish the larvae of these parasites. However, these probes have a short shelf-life and are hazardous to the users. Two oligonucleotide DNA probes have been tested, one is specific for B.malayi and the other specific for B.pahangi. They were each labeled with Biotin in three different ways by using : a one-tailed 30mer biotinylated uridine residues, a two-tailed 30mer biotinylated uridine-thymidine residues. The dot blot assays were tested at various temperatures (30°C-80°C) using different concentrations of parasite DNAs (12.8ng-0.1ng). Our preliminary results indicated that the sensitivity and specificity of the biotinylated DNA probes, with a two-tailed 45mer biotinylated residues, were highly acceptable for field use

Corticosterone Acts in the Nucleus Accumbens to Enhance Dopamine Signaling and Potentiate Reinstatement of Cocaine Seeking

Stressful life events are important contributors to relapse in recovering cocaine addicts, but the mechanisms by which they influence motivational systems are poorly understood. Studies suggest that stress may “set the stage” for relapse by increasing the sensitivity of brain reward circuits to drug-associated stimuli. We examined the effects of stress and corticosterone on behavioral and neurochemical responses of rats to a cocaine prime after cocaine self-administration and extinction. Exposure of rats to acute electric footshock stress did not by itself reinstate drug-seeking behavior but potentiated reinstatement in response to a subthreshold dose of cocaine. This effect of stress was not observed in adrenalectomized animals, and was reproduced in nonstressed animals by administration of corticosterone at a dose that reproduced stress-induced plasma levels. Pretreatment with the glucocorticoid receptor antagonist RU38486 did not block the corticosterone effect. Corticosterone potentiated cocaine-induced increases in extracellular dopamine in the nucleus accumbens (NAc), and pharmacological blockade of NAc dopamine receptors blocked corticosterone-induced potentiation of reinstatement. Intra-accumbens administration of corticosterone reproduced the behavioral effects of stress and systemic corticosterone. Corticosterone treatment acutely decreased NAc dopamine clearance measured by fast-scan cyclic voltammetry, suggesting that inhibition of uptake2-mediated dopamine clearance may underlie corticosterone effects. Consistent with this hypothesis, intra-accumbens administration of the uptake2 inhibitor normetanephrine potentiated cocaine-induced reinstatement. Expression of organic cation transporter 3, a corticosterone-sensitive uptake2 transporter, was detected on NAc neurons. These findings reveal a novel mechanism by which stress hormones can rapidly regulate dopamine signaling and contribute to the impact of stress on drug intake

Unified N=2 Maxwell-Einstein and Yang-Mills-Einstein Supergravity Theories in Four Dimensions

We study unified N=2 Maxwell-Einstein supergravity theories (MESGTs) and unified Yang-Mills Einstein supergravity theories (YMESGTs) in four dimensions. As their defining property, these theories admit the action of a global or local symmetry group that is (i) simple, and (ii) acts irreducibly on all the vector fields of the theory, including the ``graviphoton''. Restricting ourselves to the theories that originate from five dimensions via dimensional reduction, we find that the generic Jordan family of MESGTs with the scalar manifolds [SU(1,1)/U(1)] X [SO(2,n)/SO(2)X SO(n)] are all unified in four dimensions with the unifying global symmetry group SO(2,n). Of these theories only one can be gauged so as to obtain a unified YMESGT with the gauge group SO(2,1). Three of the four magical supergravity theories defined by simple Euclidean Jordan algebras of degree 3 are unified MESGTs in four dimensions. Two of these can furthermore be gauged so as to obtain 4D unified YMESGTs with gauge groups SO(3,2) and SO(6,2), respectively. The generic non-Jordan family and the theories whose scalar manifolds are homogeneous but not symmetric do not lead to unified MESGTs in four dimensions. The three infinite families of unified five-dimensional MESGTs defined by simple Lorentzian Jordan algebras, whose scalar manifolds are non-homogeneous, do not lead directly to unified MESGTs in four dimensions under dimensional reduction. However, since their manifolds are non-homogeneous we are not able to completely rule out the existence of symplectic sections in which these theories become unified in four dimensions.Comment: 47 pages; latex fil

A Field Study Using the Polymerase Chain Reaction (Pcr) to Screen for Brugia Microfilariae in Human and Animal Blood

Blood samples from 43 humans and 14 cats positive with Brugia microfilariae were analyzed in a field study in Tanjung Pinang, Indonesia. The study used the polymerase chain reaction (PCR) to compare the sensitivity of radioactive and biotinylated species-specific oligonuleotide probes. The cloning char­acterization of the Hha I repeat DNA family found in filarial parasites of the genus Brugia, and the development of species-specific probes for B.malayi and B.pahangi based on these repeats has been described elsewhere (PNAS USA 83: 797-801); Mol.Biochem. Parasitol. 2$: 163-170). The use of radioisotopes for labelling DNA probes is both expensive and inconvenient. To replace these probes, biotinylated DNA probes have been designed for non- radioactive detection of B.malayi and B.pahangi. These oligonucleotide probes have long tails of biotinylated uridine residues added to their 5\u27 end. As little as 100 pg of Brugia DNA can be detected on dot blot with these probes. Detection of the probes is based on an avidin-alkaline phosphatase colorimetric assay. In order to distinguish between infected from uninfected individuals, it is necessary to detect the amount of DNA in one microfilaria (about 60 pg). The polymerase chain reaction (PCR) is a procedure in which a small amount of DNA can be amplified up to 1 million-fold. A part of each sample in this study was PCR amplified and compared with the unamplified portion using both the radioactive and biotinylated DNA probe. The PCR amplified samples were accurately identified by both the radioactive and biotinylated B.malayi and B.pahangi probes. Even samples with as few as two microfilariae per lOOul of blood were easily detected. The samples that were not PCR amplified were accurately identified after only long exposures (greater than one week) to the radioactive probes. The biotinylated probes, were not sensitive enough for accurate identification of the non-PCR amplified samples. The polymerase chain reaction is, therefore, a promising new tool for enhancing the sensitivity of parasite detection assays based on DNA probes. This will be especially important in designing assay based on non-radioactive DNA probes

Reduction of Campylobacter and Salmonella in pigs treated with A select nitrocompound

The aim of this study was to test the effectiveness of administering a select nitrocompound (S-NO) on reducing naturally colonized Campylobacter and experimentally infected Salmonella in the weaned pig gut. Pigs were divided into four groups; control (0 g S-NO/pig), 1X (0.2 g S-NO/pig), 5X (1 g S-NO/pig), and 10X (2 g S-NO/pig). Treatments were administered via oral gavage 24 h before sacrifice. Mean ± SD populations (log10 cfu/g) of Campylobacter in the cecum were reduced (P \u3c 0.05) in pigs receiving the 10X dose when compared with untreated controls (1.64 ± 1.30 vs 5.31 ± 0.58, respectively). Campylobacter concentrations in rectal contents from pigs administered the 5X dose were reduced (P \u3c 0.05) compared to control (2.65 ± 2.86 vs 5.90 ± 0.94, respectively). Rectal Salmonella concentrations were reduced (P \u3c 0.05) in all of the S-NO-treated groups. Adverse effects of S-NO on pig health were not observed. These results demonstrate that S-NO may have potential as an intervention to reduce pig colonization by Campylobacter and Salmonella

Event-based camera refractory period characterization and initial clock drift evaluation

Event-based camera (EBC) technology provides high-dynamic range operation and shows promise for efficient capture of spatio-temporal information, producing a sparse data stream and enabling consideration of nontraditional data processing solutions (e.g., new algorithms, neuromorphic processors, etc.). Given the fundamental difference in camera architecture, the EBC response and noise behavior differ considerably compared to standard CCD/CMOS framing sensors. These differences necessitate the development of new characterization techniques and sensor models to evaluate hardware performance and elucidate the trade-space between the two camera architectures. Laboratory characterization techniques reported previously include noise level as a function of static scene light level (background activity) and contrast responses referred to as S-curves. Here we present further progress on development of basic characterization methods and test capabilities for commercial-off-the-shelf (COTS) visible EBCs, with a focus on measurement of pixel deadtime (refractory period) including results for the 4th-generation sensor from Prophesee and Sony. Refractory period is empirically determined from analysis of the interspike intervals (ISIs), and results visualized using log-histograms of the minimum per-pixel ISI values for a subset of pixels activated by a controlled dynamic scene. Our tests of the Prophesee gen4 EVKv2 yield refractory period estimates ranging from 6.1 msec to 6.8 μsec going from the slowest (20) to fastest (100) settings of the relevant bias parameter, bias_refr. We also introduce and demonstrate the concept of pixel bandwidth measurement from data captured while viewing a static scene – based on recording data at a range of refractory period setting and then analyzing noise-event statistics. Finally, we present initial results for estimating and correcting EBC clock drift using a GPS PPS signal to generate special timing events in the event-list data streams generated by the DAVIS346 and DVXplorer EBCs from iniVation

Counting and effective rigidity in algebra and geometry

The purpose of this article is to produce effective versions of some rigidity results in algebra and geometry. On the geometric side, we focus on the spectrum of primitive geodesic lengths (resp., complex lengths) for arithmetic hyperbolic 2-manifolds (resp., 3-manifolds). By work of Reid, this spectrum determines the commensurability class of the 2-manifold (resp., 3-manifold). We establish effective versions of these rigidity results by ensuring that, for two incommensurable arithmetic manifolds of bounded volume, the length sets (resp., the complex length sets) must disagree for a length that can be explicitly bounded as a function of volume. We also prove an effective version of a similar rigidity result established by the second author with Reid on a surface analog of the length spectrum for hyperbolic 3-manifolds. These effective results have corresponding algebraic analogs involving maximal subfields and quaternion subalgebras of quaternion algebras. To prove these effective rigidity results, we establish results on the asymptotic behavior of certain algebraic and geometric counting functions which are of independent interest.Comment: v.2, 39 pages. To appear in Invent. Mat