Trends in High-Risk HLA Susceptibility Genes Among Colorado Youth With Type 1 Diabetes

OBJECTIVE—Type 1 diabetes is associated with a wide spectrum of susceptibility and protective genotypes within the HLA class II system. It has been reported that adults diagnosed with youth-onset type 1 diabetes more recently have been found to have fewer classical high-risk HLA class II genotypes than those diagnosed several decades ago. We hypothesized that such temporal trends in the distribution of HLA-DR, DQ genotypes would be evident, and perhaps even stronger, among 5- to 17-year-old Hispanic and non-Hispanic white (NHW) youth diagnosed with type 1 diabetes in Colorado between 1978 and 2004

Arab Springs Making Space: Territoriality and Moral Geographies for Asylum Seekers in Italy

This paper engages the instabilities of the category of ‘asylum’ looking at Arab Uprisings' mobility to Italy and highlighting the contested encounter between governmental practices in managing asylum seekers and the embodied experiences of mobility from Libya and Tunisia to Italy in 2011–12. Focusing on asylum application processing, our analysis tackles its sorting rationalities (asylum seekers versus irregular migrants, country of birth versus country of refuge), its produced spatialities (processing centers and ‘humanitarian emergency zones’), and its moral predicaments (how vulnerability and protection are put to work). Our aim is to contribute to a political epistemology of asylum whereby asylum's normative instabilities are mobilized to trouble its exclusionary boundaries and the profiling of the refugee as the alter ego figure of the citizen. The paper revolves around four episodes: it opens attending to an epistemological challenge; it stages a critical engagement with the Italian ‘North Africa Emergency’ in the second and third section; finally, it puts this analysis to work on the terrain of a political struggle to demand a right to presence for Libyan war evacuees

A robust nitrifying community in a bioreactor at 50 °C opens up the path for thermophilic nitrogen removal

The increasing production of nitrogen-containing fertilizers is crucial to meet the global food demand, yet high losses of reactive nitrogen associated with the food production/consumption chain progressively deteriorate the natural environment. Currently, mesophilic nitrogen-removing microbes eliminate nitrogen from wastewaters. Although thermophilic nitrifiers have been separately enriched from natural environments, no bioreactors are described that couple these processes for the treatment of nitrogen in hot wastewaters. Samples from composting facilities were used as inoculum for the batch-wise enrichment of thermophilic nitrifiers (350 days). Subsequently, the enrichments were transferred to a bioreactor to obtain a stable, high-rate nitrifying process (560 days). The community contained up to 17% ammonia-oxidizing archaea (AOAs) closely related to 'Candidatus Nitrososphaera gargensis', and 25% nitrite-oxidizing bacteria (NOBs) related to Nitrospira calida. Incorporation of C-13-derived bicarbonate into the respective characteristic membrane lipids during nitrification supported their activity as autotrophs. Specific activities up to 198 +/- 10 and 894 +/- 81 mg N g(-1) VSS per day for AOAs and NOBs were measured, where NOBs were 33% more sensitive to free ammonia. The NOBs were extremely sensitive to free nitrous acid, whereas the AOAs could only be inhibited by high nitrite concentrations, independent of the free nitrous acid concentration. The observed difference in product/substrate inhibition could facilitate the development of NOB inhibition strategies to achieve more cost-effective processes such as deammonification. This study describes the enrichment of autotrophic thermophilic nitrifiers from a nutrient-rich environment and the successful operation of a thermophilic nitrifying bioreactor for the first time, facilitating opportunities for thermophilic nitrogen removal biotechnology