Utah\u27s Rural Communities: Planning for the Future

Two of the biggest concerns facing rural communities in the Intermountain West today are the contrasting problems of rapid growth and development as opposed to economic decline and stagnation

Utah\u27s Great Outdoor Open Space Project

As our populations increase, and more and more development takes place, critical lands and waters are threatened or even lost in the ensuing rush for economic progress

Development and Field Evaluation of an Aquifer Stratification Testing System

An aquifer stratification testing system was designed, constructed, and tested. The system was designed to characterize vertical profiles of both quantity and quality of water produced from actively pumping groundwater wells. During testing, all systems functioned properly and either met or exceeded anticipated performance requirements. The system was first tested in an irrigation well completed in the Edwards aquifer and owned by the Onion Creek Club located in Manchaca, Texas, just south of Austin. The purpose of the exercise was to assess test systems performance and to develop and refine testing procedures. Dye-tracer injection tests were performed at five depths from 325 to 405 ft, and a velocity/discharge profile was generated for that interval. The profile was consistent with the karst nature of the Edwards aquifer. Water sampling procedures were tested and evaluated, but no samples were analyzed. Further testing was performed in two public water supply wells completed in the Ogallala Formation of the High Plains aquifer and belonging to the City of Wolfforth, Texas, to investigate arsenic and fluoride concentration distributions, which both exceed maximum contaminant level (MCL) standards for arsenic (10 μg/L) and fluoride (4 mg/L) in produced water. The results indicated vertical trends in both arsenic and fluoride aquifer concentration profiles, although concentrations at all depths exceeded MCL standards for both arsenic and fluoride. Despite distinctly different water discharge profiles between wells, a consistent inverse depth relationship between arsenic and fluoride concentrations was identified in both wells. Aquifer arsenic concentrations generally decreased, and aquifer fluoride concentrations generally increased with increasing depth. Aquifer arsenic concentrations ranged from 11.4 to 14.1 μg/L in Well 8 and from 16.0 to 22.2 μg/L in Well 5. Aquifer fluoride concentrations ranged from 5.53 to 7.98 mg/L in Well 8 and from 4.28 to 5.87 mg/L in Well 5. Future system and procedural modifications should focus on improving and enhancing the flexibility and data acquisition capabilities of the system. Future field testing programs should be performed to develop a water quality stratification database to enhance general understanding of groundwater quality and flow processes and provide insight on potential contamination sources.Bureau of Economic Geolog

Antifungal Susceptibility Profiles of 1698 Yeast Reference Strains Revealing Potential Emerging Human Pathogens

New molecular identification techniques and the increased number of patients with various immune defects or underlying conditions lead to the emergence and/or the description of novel species of human and animal fungal opportunistic pathogens. Antifungal susceptibility provides important information for ecological, epidemiological and therapeutic issues. The aim of this study was to assess the potential risk of the various species based on their antifungal drug resistance, keeping in mind the methodological limitations. Antifungal susceptibility profiles to the five classes of antifungal drugs (polyens, azoles, echinocandins, allylamines and antimetabolites) were determined for 1698 yeast reference strains belonging to 992 species (634 Ascomycetes and 358 Basidiomycetes). Interestingly, geometric mean minimum inhibitory concentrations (MICs) of all antifungal drugs tested were significantly higher for Basidiomycetes compared to Ascomycetes (p<0.001). Twenty four strains belonging to 23 species of which 19 were Basidiomycetes seem to be intrinsically “resistant” to all drugs. Comparison of the antifungal susceptibility profiles of the 4240 clinical isolates and the 315 reference strains belonging to 53 shared species showed similar results. Even in the absence of demonstrated in vitro/in vivo correlation, knowing the in vitro susceptibility to systemic antifungal agents and the putative intrinsic resistance of yeast species present in the environment is important because they could become opportunistic pathogens

Transistors

Contains reports on eight research projects.Lincoln Laboratory under Contract AF19(122)-45

Why highly expressed proteins evolve slowly

Much recent work has explored molecular and population-genetic constraints on the rate of protein sequence evolution. The best predictor of evolutionary rate is expression level, for reasons which have remained unexplained. Here, we hypothesize that selection to reduce the burden of protein misfolding will favor protein sequences with increased robustness to translational missense errors. Pressure for translational robustness increases with expression level and constrains sequence evolution. Using several sequenced yeast genomes, global expression and protein abundance data, and sets of paralogs traceable to an ancient whole-genome duplication in yeast, we rule out several confounding effects and show that expression level explains roughly half the variation in Saccharomyces cerevisiae protein evolutionary rates. We examine causes for expression's dominant role and find that genome-wide tests favor the translational robustness explanation over existing hypotheses that invoke constraints on function or translational efficiency. Our results suggest that proteins evolve at rates largely unrelated to their functions, and can explain why highly expressed proteins evolve slowly across the tree of life.Comment: 40 pages, 3 figures, with supporting informatio

Polycystic kidney disease: an unrecognized emerging infectious disease?

Polycystic kidney disease (PKD) is one of the most common genetic diseases in humans. We contend that it may be an emerging infectious disease and/or microbial toxicosis in a vulnerable human subpopulation. Use of a differential activation protocol for the Limulus amebocyte lysate (LAL) assay showed bacterial endotoxin and fungal (1-->3)-beta-D-glucans in cyst fluids from human kidneys with PKD. Fatty acid analysis of cyst fluid confirmed the presence of 3-hydroxy fatty acids characteristic of endotoxin. Tissue and cyst fluid from three PKD patients were examined for fungal components. Serologic tests showed Fusarium, Aspergillus, and Candida antigens. IgE, but not IgG, reactive with Fusarium and Candida were also detected in cyst fluid. Fungal DNA was detected in kidney tissue and cyst fluid from these three PKD patients, but not in healthy human kidney tissue. We examine the intertwined nature of the actions of endotoxin and fungal components, sphingolipid biology in PKD, the structure of PKD gene products, infections, and integrity of gut function to establish a mechanistic hypothesis for microbial provocation of human cystic disease. Proof of this hypothesis will require identification of the microbes and microbial components involved and multifaceted studies of PKD cell biology