Chemotaxis Side Chamber

Brine sample exposed to a right-to-left serine gradient. Data file 2015.03.30 06-14. Data for Fig. 9 A in paper

Brine at -4C

Malene Bay brine kept overnight at -4 C with no additions. File 2015.03.28 08-11. Data for Figure 8D in paper and Video 6

Brine at +4 C

Malene Bay brine kept overnight at +4 C with the addition of 1/2 strength 2216 marine medium. File 2015.03.30 05-28. Data for Figures 8F in paper and Video 8

Malene Bay eukaryotes

Malene Bay, unmodified, showing eukaryotes. Data for Fig. 6. Data file 2015.03.25 10-1

Chemotaxis Middle Chamber

Holograms of Malene Bay brine sample exposed to a bottom-to-top serine gradient (data file 2015.03.30 06-28) Data for Fig. 9 B, C in paper and Video S9

FourierMaskNuukData

Fourier mask for data sets

FourierMaskNuukData

Fourier mask for data sets

Seawater at +4C

Malene Bay seawater kept overnight at +4 C with the addition of 1/2 strength 2216 marine medium. File 2015.03.30 05-58. Data for Figures 7 and 8E in paper and Videos 4 and 7

Schematic and images of the compact, twin-beam digital holographic microscope.

<p>(A) Schematic showing four main elements (discussed in the text): the source, the sample (specimen path is labeled <i>Spec</i>. and reference path is labeled <i>Ref</i>.), the microscope, and the sensor. (B) Solid model of the hardware. The fiber-fed source assembly is at the bottom, and the imaging camera is at the top. The microscope optics, comprised of the two aspheric lenses and the relay lens, are contained within the 300 mm long lens tube. In the laboratory, a three-axis stage between the source the microscope optics provides easy manual manipulation of the specimen under study. (C) Photograph of the field instrument (top case removed). The optical train, electronics, and computer are contained within a waterproof box. (D) Photo of instrument fully enclosed, as used in the field. The arrow indicates where a sample chamber is inserted; the structure pictured is a placeholder only.</p

Images by epifluorescence microscopy of brine samples.

<p>Organisms are stained with acridine orange and DAPI according to a dual-staining technique [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147700#pone.0147700.ref041" target="_blank">41</a>] and showed an assemblage of diatoms (A, C) and flagellates (B, D) from Malene Bay (A, B) and Kobbefjord (C, D). All scale bars are 10 μm. From Malene Bay: (A) A diatom, probably <i>Navicula vanhoeffenii</i> and (B) a flagellate, possibly <i>Chlamydomonas</i> or <i>Telonemia</i>; from Kobbefjord: (C) a diatom, probably <i>Fragilariopsis oceanica</i>, and (D) a flagellate, <i>Pyraminmonas sp</i>. <i>(</i><a href="http://westerndiatoms.colorado.edu/" target="_blank">http://westerndiatoms.colorado.edu/</a>) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147700#pone.0147700.ref046" target="_blank">46</a>] [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147700#pone.0147700.ref047" target="_blank">47</a>].</p