13 research outputs found
Precise non-destructive X-ray fluorescence method for uranium and thorium concentration measurements
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Modeling long QT syndrome type 2 on-a-chip via indepth assessment of isogenic gene-edited 3D cardiac tissues
Long QT syndrome (LQTS) is a cardiovascular disease characterized by QT interval prolongation that can lead to sudden cardiac death. Many mutations with heterogeneous mechanisms have been identified in KCNH2, the gene that encodes for hERG (Kv11.1), which lead to onset of LQTS type 2 (LQTS2). In this work, we developed a LQTS2-diseased tissue-on-a-chip model, using 3D coculture of isogenic stem cell-derived cardiomyocytes (CMs) and cardiac fibroblasts (CFs) within an organotypic microfluidic chip technology. Primarily, we created a hiPSC line with R531W mutation in KCNH2 using CRISPR-Cas9 gene-editing technique and characterized the resultant differentiated CMs and CFs. A deficiency in hERG trafficking was identified in KCNH2-edited hiPSC-CMs, revealing a possible mechanism of R531W mutation in LQTS2 pathophysiology. Following creation of a 3D LQTS2 tissue-on-a-chip, the tissues were extensively characterized, through analysis of calcium handling and response to β-agonist. Furthermore, attempted phenotypic rescue via pharmacological intervention of LQTS2 on a chip was investigated. © 2022 The Authors.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
PEGylated-nanoliposomal clusterin for amyloidogenic light chain-induced endothelial dysfunction
Selective Recovery of Vanadium by Oxy-carbochlorination of Basic Oxygen Furnace Slag: Experimental Study
Cinza da lenha para aplicação em cerâmica vermelha. parte I: caracterÃsticas da cinza Firewood ash for application in red ceramic. part I: characteristics of the ash
A lenha de eucalipto constitui-se no principal combustÃvel utilizado pelas indústrias de cerâmica vermelha do municÃpio de Campos dos Goytacazes, Estado do Rio de Janeiro. Em princÃpio, esta lenha tem caracterÃsticas promissoras para adição em cerâmica. Assim, esse trabalho tem por objetivo caracterizar a cinza proveniente da combustão de lenha predominantemente de eucalipto visando a incorporação na massa de cerâmica vermelha processada em indústrias de Campos dos Goytacazes. Foram realizados ensaios de fluorescência de raios X, difração de raios X, distribuição de tamanho de partÃcula, análise térmica (ATD/TG), porosimetria de mercúrio e microscopia eletrônica de varredura. Os resultados mostraram que a cinza da lenha se apresenta como aglomerados de partÃculas, sendo constituÃda principalmente por Ca, Si, Mg, K e S.<br>The eucalyptus firewood is one of the main fuels used by the industries of red ceramic in the municipal area of Campos of Goytacazes, State of Rio de Janeiro. In principle this firewood has promising characteristics for addition into ceramics. Thus, the present work has for objective to characterize the ash generated from burning firewood aiming at its incorporation into red ceramic products. The ash was submitted to X-ray diffraction, chemical composition, particle size distribution, thermal analysis (ATD/TG), mercury porosimetry and scanning electron microscopy tests. The results showed that the ash of the firewood comes as agglomerates of particles, being constituted mainly by Ca, Si, Mg, K and S