Mission design for LISA Pathfinder

Here we describe the mission design for SMART-2/LISA Pathfinder. The best trade-off between the requirements of a low-disturbance environment and communications distance is found to be a free-insertion Lissajous orbit around the first co-linear Lagrange point of the Sun-Earth system L1, 1.5x 10^6 km from Earth. In order to transfer SMART-2/LISA Pathfinder from a low Earth orbit, where it will be placed by a small launcher, the spacecraft carries out a number of apogee-raise manoeuvres, which ultimatively place it to a parabolic escape trajectory towards L1. The challenges of the design of a small mission are met, fulfilling the very demanding technology demonstration requirements without creating excessive requirements on the launch system or the ground segment.Comment: 7 pages, 6 figures, 5th International LISA Symposium, see http://www.landisoft.de/Markus-Landgra

Doppler imaging of the helium-variable star a Cen

The helium-peculiar star a Cen exhibits line profile variations of elements such as iron, nitrogen and oxygen in addition to its well-known extreme helium variability. New high S/N, high-resolution spectra are used to perform a quantitative measurement of the abundances of the star and determine the relation of the concentrations of the heavier elements on the surface of the star to the helium concentration and the magnetic field orientation. Doppler images have been created using programs described in earlier papers by Rice and others. An alternative surface abundance mapping code has been used to model the helium line variations after our Doppler imaging of certain individual helium lines produced mediocre results. We confirm the long-known existence of helium-rich and helium-poor hemispheres on a Cen and we measure a difference of more than two orders of magnitude in helium abundance from one side of the star to the other. Helium is overabundant by a factor of about 5 over much of the helium-rich hemisphere. Of particular note is our discovery that the helium-poor hemisphere has a very high abundance of helium-3, approximately equal to the helium-4 abundance. a Cen is therefore a new member of the small group of helium-3 stars and the first well-established magnetic member of the class. For the three metals investigated here, there are two strong concentrations of abundance near the equator consistent with the positive magnetic maximum and two somewhat weaker concentrations of abundance where the helium concentration is centered and roughly where the negative peak of the magnetic field would be found. Another strong concentration is found near the equator and this is not explainable in terms of any simple symmetry with the helium abundance or the apparent magnetic field main polar locations.Comment: 9 pages, 9 figure

A cross-national study of school students' perceptions of political messages in two national election campaigns

The aim of this study is to understand, qualitatively, the ways in which school students from England and the US engaged with campaign messages during two national elections. We combined data from eight focus groups conducted in four schools in England (2 primary/elementary and 2 secondary/high; N = 60) and individual interviews conducted in one school in the US (N=19). Three common themes were identified from our cross-national data: the trustworthiness of candidates, equality, and the narrative surrounding immigration. We argue that it is important for students to have spaces to understand, explore and debate political messages and for teachers to feel confident and able to engage with those campaign messages especially where they may be controversial, partisan or challenging

MHCII-independent CD4(+) T cells protect injured CNS neurons via IL-4

A body of experimental evidence suggests that T cells mediate neuroprotection following CNS injury; however, the antigen specificity of these T cells and how they mediate neuroprotection are unknown. Here, we have provided evidence that T cell-mediated neuroprotection after CNS injury can occur independently of major histocompatibility class II (MHCII) signaling to T cell receptors (TCRs). Using two murine models of CNS injury, we determined that damage-associated molecular mediators that originate from injured CNS tissue induce a population of neuroprotective, IL-4-producing T cells in an antigen-independent fashion. Compared with wild-type mice, IL-4-deficient animals had decreased functional recovery following CNS injury; however, transfer of CD4+ T cells from wild-type mice, but not from IL-4-deficient mice, enhanced neuronal survival. Using a culture-based system, we determined that T cell-derived IL-4 protects and induces recovery of injured neurons by activation of neuronal IL-4 receptors, which potentiated neurotrophin signaling via the AKT and MAPK pathways. Together, these findings demonstrate that damage-associated molecules from the injured CNS induce a neuroprotective T cell response that is independent of MHCII/TCR interactions and is MyD88 dependent. Moreover, our results indicate that IL-4 mediates neuroprotection and recovery of the injured CNS and suggest that strategies to enhance IL-4-producing CD4+ T cells have potential to attenuate axonal damage in the course of CNS injury in trauma, inflammation, or neurodegeneration

P2Y₁₂-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction

Emergency hematopoiesis is the driving force of the inflammatory response to myocardial infarction (MI). Increased proliferation of hematopoietic stem and progenitor cells (LSK) after MI enhances cell production in the bone marrow (BM) and replenishes leukocyte supply for local cell recruitment to the infarct. Decoding the regulation of the inflammatory cascade after MI may provide new avenues to improve post-MI remodeling. In this study, we describe the influence of adenosine diphosphate (ADP)-dependent P2Y12-mediated signaling on emergency hematopoiesis and cardiac remodeling after MI. Permanent coronary ligation was performed to induce MI in a murine model. BM activation, inflammatory cell composition and cardiac function were assessed using global and platelet-specific gene knockout and pharmacological inhibition models for P2Y12. Complementary in vitro studies allowed for investigation of ADP-dependent effects on LSK cells. We found that ADP acts as a danger signal for the hematopoietic BM and fosters emergency hematopoiesis by promoting Akt phosphorylation and cell cycle progression. We were able to detect P2Y12 in LSK, implicating a direct effect of ADP on LSK via P2Y12 signaling. P2Y12 knockout and P2Y12 inhibitor treatment with prasugrel reduced emergency hematopoiesis and the excessive inflammatory response to MI, translating to lower numbers of downstream progeny and inflammatory cells in the blood and infarct. Ultimately, P2Y12 inhibition preserved cardiac function and reduced chronic adverse cardiac remodeling after MI. P2Y12-dependent signaling is involved in emergency hematopoiesis after MI and fuels post-ischemic inflammation, proposing a novel, non-canonical value for P2Y12 antagonists beyond inhibition of platelet-mediated atherothrombosis

The P2Y1 receptor is involved in the maintenance of glucose homeostasis and in insulin secretion in mice

Pancreatic β cells express several P2 receptors including P2Y1 and the modulation of insulin secretion by extracellular nucleotides has suggested that these receptors may contribute to the regulation of glucose homeostasis. To determine whether the P2Y1 receptor is involved in this process, we performed studies in P2Y1 mice. In baseline conditions, P2Y1-mice exhibited a 15% increase in glycemia and a 40% increase in insulinemia, associated with a 10% increase in body weight, pointing to a role of the P2Y1 receptor in the control of glucose metabolism. Dynamic experiments further showed that P2Y1-mice exhibited a tendency to glucose intolerance. These features were associated with a decrease in the plasma levels of free fatty acid and triglycerides. When fed a lipids and sucrose enriched diet for 15 weeks, the two genotypes no longer displayed any significant differences. To determine whether the P2Y1 receptor was directly involved in the control of insulin secretion, experiments were carried out in isolated Langerhans islets. In the presence of high concentrations of glucose, insulin secretion was significantly greater in islets from P2Y1-mice. Altogether, these results show that the P2Y1 receptor plays a physiological role in the maintenance of glucose homeostasis at least in part by regulating insulin secretion