The formation of secondary organic aerosol from the isoprene + OH reaction in the absence of NO_x

The reaction of isoprene (C₅H₈) with hydroxyl radicals has been studied in the absence of nitrogen oxides (NO_x) to determine physical and chemical characteristics of the secondary organic aerosol formed. Experiments were conducted using a smog chamber operated in a steady-state mode permitting measurements of moderately low aerosol levels. GC-MS analysis was conducted to measure methyl butenediols in the gas phase and polyols in the aerosol phase. Analyses were made to obtain several bulk aerosol parameters from the reaction including values for the organic mass to organic carbon ratio, the effective enthalpy of vaporization (ΔH_vap^eff), organic peroxide fraction, and the aerosol yield. <br><br> The gas phase analysis showed the presence of methacrolein, methyl vinyl ketone, and four isomers of the methyl butenediols. These gas-phase compounds may serve as precursors for one or more of several compounds detected in the aerosol phase including 2-methylglyceric acid, three 2-methyl alkenetriols, and two 2-methyl tetrols. In contrast to most previous studies, the 2-methyl tetrols (and the 2-methyl alkenetriols) were found to form in the absence of acidic sulfate aerosol. However, reaction conditions did not favor the production of HO₂ radicals, thus allowing RO₂+RO₂ reactions to proceed more readily than if higher HO₂ levels had been generated. <br><br> SOA/SOC (i.e. OM/OC) was found to average 1.9 in the absence of NO_x. The effective enthalpy of vaporization was measured as 38.6 kJ mol^&minus;1, consistent with values used previously in modeling studies. The yields in this work (using an independent technique than used previously) are lower than those of Kroll et al. (2006) for similar aerosol masses. SOC yields reported in this work range from 0.5–1.4% for carbon masses between 17 and 49 μgC m^&minus;3

Determinación de la actividad endo-proteolítica en cebada malteada = Endoproteolytic activity assay in malting barley

La hidrólisis de las proteínas de cebada en péptidos y aminoácidos es uno de los procesos más importantes en la germinación de la cebada. La degradación de las proteínas de reserva del endosperma promueven tanto el aumento de la concentración de nitrógeno amínico como la modificación del endosperma, facilitando la movilidad del agua y, por ende, de las enzimas. La actividad proteolítica es el resultado de actividades conjuntas de exo- y endo-peptidasas. Las proteínas de cebada son solubilizadas inicialmente por las endo-peptidasas y luego por las exo-peptidasas. Se han descrito cuatro tipos de endo-peptidasas: serin, cisteín, aspartic y metalo. El objetivo del trabajo consistió en la puesta a punto de un método enzimático para determinar la actividad endo-proteolítica de estas cuatro enzimas, utilizando un método rápido, colorimétrico, con dos sustratos diferentes: azo-gelatina y azo-caseína. Se optimizaron las condiciones de ensayo: pH, tiempo y temperatura de reacción. El sustrato azo-gelatina presentóvarias dificultades para estandarizar un método colorimétrico que sea reproducible. En cambio, el sustrato azo-caseína fue muy consistente y permitió realizar las curvas de estandarización para las cuatro enzimas. Luego el método se aplicó exitosamente para la determinación de la actividad endo-proteolítica en cebada, malta y mosto cervecero

Influence of aerosol acidity on the chemical composition of secondary organic aerosol from β-caryophyllene

The secondary organic aerosol (SOA) yield of β-caryophyllene photooxidation is enhanced by aerosol acidity. In the present study, the influence of aerosol acidity on the chemical composition of β-caryophyllene SOA is investigated using ultra performance liquid chromatography/electrospray ionization-time-of-flight mass spectrometry (UPLC/ESI-TOFMS). A number of first-, second- and higher-generation gas-phase products having carbonyl and carboxylic acid functional groups are detected in the particle phase. Particle-phase reaction products formed via hydration and organosulfate formation processes are also detected. Increased acidity leads to different effects on the abundance of individual products; significantly, abundances of organosulfates are correlated with aerosol acidity. To our knowledge, this is the first detection of organosulfates and nitrated organosulfates derived from a sesquiterpene. The increase of certain particle-phase reaction products with increased acidity provides chemical evidence to support the acid-enhanced SOA yields. Based on the agreement between the chromatographic retention times and accurate mass measurements of chamber and field samples, three β-caryophyllene products (i.e., β-nocaryophyllon aldehyde, β-hydroxynocaryophyllon aldehyde, and β-dihydroxynocaryophyllon aldehyde) are suggested as chemical tracers for β-caryophyllene SOA. These compounds are detected in both day and night ambient samples collected in downtown Atlanta, GA and rural Yorkville, GA during the 2008 August Mini-Intensive Gas and Aerosol Study (AMIGAS)

Microclimate and resource quality determine resource use in a range-expanding herbivore

This is the author accepted manuscript. The final version is available from the Royal Society via the DOI in this recordData accessibility: The supporting data are available via the Dryad repository at https://doi.org/10.5061/dryad.3ffbg79j1 [46]. The supporting R code is available via Zenodo at https://doi.org/10.5281/zenodo.4898844 [47]. The data are provided in the electronic supplementary material [67].The consequences of climate change for biogeographic range dynamics depend on the spatial scales at which climate influences focal species directly and indirectly via biotic interactions. An overlooked question concerns the extent to which microclimates modify specialist biotic interactions, with emergent properties for communities and range dynamics. Here, we use an in-field experiment to assess egg-laying behaviour of a range-expanding herbivore across a range of natural microclimatic conditions. We show that variation in microclimate, resource condition and individual fecundity can generate differences in egg-laying rates of almost two orders of magnitude in an exemplar species, the brown argus butterfly (Aricia agestis). This within-site variation in fecundity dwarfs variation resulting from differences in average ambient temperatures among populations. Although higher temperatures did not reduce female selection for host plants in good condition, the thermal sensitivities of egg-laying behaviours have the potential to accelerate climate-driven range expansion by increasing egg-laying encounters with novel hosts in increasingly suitable microclimates. Understanding the sensitivity of specialist biotic interactions to microclimatic variation is, therefore, critical to predict the outcomes of climate change across species' geographical ranges, and the resilience of ecological communities.Natural Environment Research Council (NERC

Energy research within the UNFCCC: a proposal to guard against ongoing climate-deadlock

We propose that an international ‘Low-Emissions Technology Commitment’ should be incorporated into the United Nations Framework Convention on Climate Change (UNFCCC) negotiation process in order to promote innovation that will enable deep decarbonization. The goal is to accelerate research, development, and demonstration of safe, scalable, and affordable low-emissions energy technologies. Such a commitment should be based on three elements. First, it should operate within existing UNFCCC negotiations so as to encourage developed states to offer directed funding for energy research as part of their national contributions. Second, pledges should be binding, verifiable, and coordinated within an international energy-research plan. Third, expert scientific networks and participating governments should collaborate to design a coordinated global research and technology-demonstration strategy and oversee national research efforts. To this end an Intergovernmental Panel on Low-Emissions Technology Research might be established. This proposal offers some insurance against the risk that the political impasse in international negotiations cannot be overcome. The higher costs associated with low-emissions alternatives to fossil fuels currently creates significant economic and political resistance to their widespread adoption. To breach this impasse, a mechanism supporting accelerated energy research is needed that seeks to reduce future abatement costs, share experience and ‘learning-by-doing’ in first-of-a-kind demonstrations, and thus facilitate future widespread deployments. These actions will also assist in addressing inequalities in energy access