362 research outputs found
Ion-channel-like behavior in lipid bilayer membranes at the melting transition
It is well known that at the gel-liquid phase transition temperature a lipid
bilayer membrane exhibits an increased ion permeability. We analyze the
quantized currents in which the increased permeability presents itself. The
open time histogram shows a "-3/2" power law which implies an open-closed
transition rate that decreases like as time evolves. We
propose a "pore freezing" model to explain the observations. We discuss how
this model also leads to the noise that is commonly observed in
currents across biological and artificial membranes.Comment: 5 pages, 4 figure
Mammalian sugar-binding receptors: known functions and unexplored roles
Mammalian glycan-binding receptors, sometimes known as lectins, interact withglycans, the oligosaccharide portions ofendogenous mammalian glycoproteins and glycolipids as well as sugars on the surfaces of microbes.Thesereceptors guide glycoproteins out of and back into cells, facilitate communication between cells through both adhesion and signaling, and allow the innate immune system to respond quickly to viral, fungal, bacterial,and parasiticpathogens.For many of the roughly one hundred glycan-binding receptors that are known in humans,there aregood descriptions of what types of glycans they bind and how selectivity for these ligands is achievedat the molecular level.In some cases, there is also comprehensive evidencefor the roles thatthe receptors playat the cellular and organismal levels. In addition to highlighting these well-understood paradigms for glycan-binding receptors, this review will suggest where gaps remain in our understandingof the physiological functions that they can serve
Phase transitions in biological membranes
Native membranes of biological cells display melting transitions of their
lipids at a temperature of 10-20 degrees below body temperature. Such
transitions can be observed in various bacterial cells, in nerves, in cancer
cells, but also in lung surfactant. It seems as if the presence of transitions
slightly below physiological temperature is a generic property of most cells.
They are important because they influence many physical properties of the
membranes. At the transition temperature, membranes display a larger
permeability that is accompanied by ion-channel-like phenomena even in the
complete absence of proteins. Membranes are softer, which implies that
phenomena such as endocytosis and exocytosis are facilitated. Mechanical signal
propagation phenomena related to nerve pulses are strongly enhanced. The
position of transitions can be affected by changes in temperature, pressure, pH
and salt concentration or by the presence of anesthetics. Thus, even at
physiological temperature, these transitions are of relevance. There position
and thereby the physical properties of the membrane can be controlled by
changes in the intensive thermodynamic variables. Here, we review some of the
experimental findings and the thermodynamics that describes the control of the
membrane function.Comment: 23 pages, 15 figure
From supported membranes to tethered vesicles: lipid bilayers destabilisation at the main transition
We report results concerning the destabilisation of supported phospholipid
bilayers in a well-defined geometry. When heating up supported phospholipid
membranes deposited on highly hydrophilic glass slides from room temperature
(i.e. with lipids in the gel phase), unbinding was observed around the main gel
to fluid transition temperature of the lipids. It lead to the formation of
relatively monodisperse vesicles, of which most remained tethered to the
supported bilayer. We interpret these observations in terms of a sharp decrease
of the bending rigidity modulus in the transition region, combined
with a weak initial adhesion energy. On the basis of scaling arguments, we show
that our experimental findings are consistent with this hypothesis.Comment: 11 pages, 3 figure
Profiling of Glycan Receptors for Minute Virus of Mice in Permissive Cell Lines Towards Understanding the Mechanism of Cell Recognition
The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 GalÎČ1-4GlcNAc motif (Neu5Acα2-3GalÎČ1-4GlcNAc or 3âČSIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3âČSIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3âČSIA-LeX identified in a previous glycan microarray screen
The influence of anesthetics, neurotransmitters and antibiotics on the relaxation processes in lipid membranes
In the proximity of melting transitions of artificial and biological
membranes fluctuations in enthalpy, area, volume and concentration are
enhanced. This results in domain formation, changes of the elastic constants,
changes in permeability and slowing down of relaxation processes. In this study
we used pressure perturbation calorimetry to investigate the relaxation time
scale after a jump into the melting transition regime of artificial lipid
membranes. This time corresponds to the characteristic rate of domain growth.
The studies were performed on single-component large unilamellar and
multilamellar vesicle systems with and without the addition of small molecules
such as general anesthetics, neurotransmitters and antibiotics. These drugs
interact with membranes and affect melting points and profiles. In all systems
we found that heat capacity and relaxation times are related to each other in a
simple manner. The maximum relaxation time depends on the cooperativity of the
heat capacity profile and decreases with a broadening of the transition. For
this reason the influence of a drug on the time scale of domain formation
processes can be understood on the basis of their influence on the heat
capacity profile. This allows estimations of the time scale of domain formation
processes in biological membranes.Comment: 12 pages, 6 figure
Volume-energy correlations in the slow degrees of freedom of computer-simulated phospholipid membranes
Constant-pressure molecular-dynamics simulations of phospholipid membranes in
the fluid phase reveal strong correlations between equilibrium fluctuations of
volume and energy on the nanosecond time-scale. The existence of strong
volume-energy correlations was previously deduced indirectly by Heimburg from
experiments focusing on the phase transition between the fluid and the ordered
gel phases. The correlations, which are reported here for three different
membranes (DMPC, DMPS-Na, and DMPSH), have volume-energy correlation
coefficients ranging from 0.81 to 0.89. The DMPC membrane was studied at two
temperatures showing that the correlation coefficient increases as the phase
transition is approached
On the action potential as a propagating density pulse and the role of anesthetics
The Hodgkin-Huxley model of nerve pulse propagation relies on ion currents
through specific resistors called ion channels. We discuss a number of
classical thermodynamic findings on nerves that are not contained in this
classical theory. Particularly striking is the finding of reversible heat
changes, thickness and phase changes of the membrane during the action
potential. Data on various nerves rather suggest that a reversible density
pulse accompanies the action potential of nerves. Here, we attempted to explain
these phenomena by propagating solitons that depend on the presence of
cooperative phase transitions in the nerve membrane. These transitions are,
however, strongly influenced by the presence of anesthetics. Therefore, the
thermodynamic theory of nerve pulses suggests a explanation for the famous
Meyer-Overton rule that states that the critical anesthetic dose is linearly
related to the solubility of the drug in the membranes.Comment: 13 pages, 8 figure
Structure of symmetric and asymmetric "ripple" phases in lipid bilayers
We reproduce the symmetric and asymmetric ``rippled'' states of
lipid membranes by Monte Carlo simulations of a coarse-grained molecular model
for lipid-solvent mixtures. The structure and properties compare favorably with
experiments. The asymmetric ripple state is characterized by a periodic array
of fully interdigitated ``defect'' lines. The symmetric ripple state maintains
a bilayer structure, but is otherwise structurally similar. The formation of
both ripple states is driven by the propensity of lipid molecules with large
head groups to exhibit splay.Comment: 4 pages, 4 figure
Interfacial Tension of the Lipid Membrane Formed from PhosphatidylcholineâDecanoic Acid and PhosphatidylcholineâDecylamine Systems
Interfacial tension has been determined for phosphatidylcholine (PC)âdecanoic acid (DA) and PCâdecylamine (DE) membranes. PC (lecithin), DA and DE were used in the experiments; the interfacial tension values of the pure components are 1.62Â ĂÂ 10â3, â2.38Â ĂÂ 10â2 and â3.88Â ĂÂ 10â2Â N/m (hypothetical values for DA and DE), respectively. The 1:1 complexes were formed during formation of PCâDA and PCâDE membranes. The following parameters describing the complexes were determined: the surface concentrations of the lipid membranes formed from these complexes, \documentclass[12pt]{minimal}
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\begin{document}\end{document}; the interfacial tensions of such membranes, Îł3; and the stability constants of these complexes, K
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