12,008 research outputs found
Mechanical properties of polyethylene terephthalate under selected conditions and methods of preparation Semiannual progress report, period ending 31 Oct. 1967
Mechanical properties of polyethylene terephthalate under selected conditions and methods of preparatio
Identification of the Genomic Insertion Site of the Thyroid Peroxidase Promoter–Cre Recombinase Transgene Using a Novel, Efficient, Next-Generation DNA Sequencing Method
Background: It can be useful to know the transgene insertion site in transgenic mice for a variety of reasons, but determining the insertion site generally is a time consuming, expensive, and laborious task. Methods: A simple method is presented to determine transgene insertion sites that combines the enrichment of a sequencing library by polymerase chain reaction (PCR) for sequences containing the transgene, followed by next-generation sequencing of the enriched library. This method was applied to determine the site of integration of the thyroid peroxidase promoter?Cre recombinase mouse transgene that is commonly used to create thyroid-specific gene deletions. Results: The insertion site was found to be between bp 12,372,316 and 12,372,324 on mouse chromosome 9, with the nearest characterized genes being Cntn5 and Jrkl, ?1.5 and 0.9?Mbp from the transgene, respectively. One advantage of knowing a transgene insertion site is that it facilitates distinguishing hemizygous from homozygous transgenic mice. Although this can be accomplished by real-time quantitative PCR, the expected Ct difference is only one cycle, which is challenging to assess accurately. Therefore, the transgene insertion site information was used to develop a 3-primer qualitative PCR assay that readily distinguishes wild type, hemizygous, and homozygous TPO-Cre mice based upon size differences of the wild type and transgenic allele PCR products. Conclusions: Identification of the genomic insertion site of the thyroid peroxidase promoter?Cre mouse transgene should facilitate the use of these mice in studies of thyroid biology.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140267/1/thy.2015.0215.pd
Negative correlation does not imply a tradeoff between growth and reproduction in California oaks
A tradeoff between growth and reproduction, often inferred from an inverse correlation between these two variables, is a fundamental paradigm of life-history evolution. Oak species provide a unique test of this relationship because different species mature acorns either in the year of pollination or in the year after pollination. This difference allows for an interspecific comparison testing whether the apparent tradeoff is causal or the result of confounding factors influencing growth and reproduction independently. Based on 13 years of data on five California oak species, we found significant negative correlations between radial growth and seed production in the three species that produce acorns the same year in which pollination occurs, but not in two species that mature acorns the year after pollination. Rainfall, which correlates positively with radial growth and correlates negatively with acorn production (based on the year of pollination), appears to be driving this pattern. We conclude that the observed negative correlations are not causal, but rather a consequence of growth and reproduction being dependent, in opposite ways, on environmental conditions. Thus, contrary to the current consensus, growth and reproduction in these species are apparently largely independent of each other. In contrast, tradeoffs between current and future reproduction appear to be much more important in the life-history evolution of these long-lived plants. We also conclude that a negative correlation does not necessarily imply a causal mechanism and should not be used as the only evidence supporting a tradeoff
Origin of Middle-Infrared Peaks in Cerium Compounds
We have demonstrated that the middle-infrared (mid-IR) peaks in the optical
conductivity spectra of Ce ( = Pd, Sn, In) can be explained by
first-principle band structure calculation with the spin-orbit interaction. The
mid-IR peak shapes in these materials are not identical to one another:
CePd, CeSn, and CeIn have a triple-peak structure, double-peak
structure and broad single-peak structure, respectively. These peaks can be
theoretically explained by the optical transition from the occupied state to
the spin-orbit splitted Ce state. This result indicates that the mid-IR
peaks originate from the simple band picture with the Ce state near the
Fermi level, not from the conventional cf hybridization gap based on the
periodic Anderson model.Comment: 5 pages, 6 figures. To be published in J. Phys. Soc. Jpn. 78(1)
(2009
Molecular Valves for Controlling Gas Phase Transport Made from Discrete Angstrom-Sized Pores in Graphene
An ability to precisely regulate the quantity and location of molecular flux
is of value in applications such as nanoscale 3D printing, catalysis, and
sensor design. Barrier materials containing pores with molecular dimensions
have previously been used to manipulate molecular compositions in the gas
phase, but have so far been unable to offer controlled gas transport through
individual pores. Here, we show that gas flux through discrete angstrom-sized
pores in monolayer graphene can be detected and then controlled using
nanometer-sized gold clusters, which are formed on the surface of the graphene
and can migrate and partially block a pore. In samples without gold clusters,
we observe stochastic switching of the magnitude of the gas permeance, which we
attribute to molecular rearrangements of the pore. Our molecular valves could
be used, for example, to develop unique approaches to molecular synthesis that
are based on the controllable switching of a molecular gas flux, reminiscent of
ion channels in biological cell membranes and solid state nanopores.Comment: to appear in Nature Nanotechnolog
On the correllation effect in Peierls-Hubbard chains
We reexamine the dimerization, the charge and the spin gaps of a half-filled
Peierls-Hubbard chain by means of the incremental expansion technique. Our
numerical findings are in significant quantitative conflict with recently
obtained results by M. Sugiura and Y. Suzumura [J. Phys. Soc. Jpn. v. 71 (2002)
697] based on a bosonization and a renormalization group method, especially
with respect to the charge gap. Their approach seems to be valid only in the
weakly correlated case.Comment: 7pages,4figures(6eps-files
- …