Effect of Treatment and Cultivar on the Ensiling of Corn Stover

Nine cultivars of corn stover selected for ethanol potential were harvested (34 to 40% dry matter) and each ensiled with six treatments: untreated, lactic acid bacteria, cell-wall degrading enzymes, sulfuric acid, bacteria-enzyme combination and enzyme-acid combination. Ensiling was carried out in vacuum-sealed bags at ~22°C for 60 d. The untreated stovers ensiled well. Lactic acid bacteria and enzyme treatments had no effect on pH, but the bacteria-enzyme combination lowered pH in some cultivars. The acid and acid-enzyme treatments had low pH values ranging from 1.3 to 1.5. Lactic acid was generally highest in the bacteria-enzyme treatment whereas acetic acid was highest for the acid treatments. The acid treatments substantially reduced hemicellulose. Potential ethanol yield on average was highest in the bacteria-enzyme treatments

Forage quality and composition measurements as predictors of ethanol yield from maize (Zea mays L.) stover

<p>Abstract</p> <p>Background</p> <p>Improvement of biofeedstock quality for cellulosic ethanol production will be facilitated by inexpensive and rapid methods of evaluation, such as those already employed in the field of ruminant nutrition. Our objective was to evaluate whether forage quality and compositional measurements could be used to estimate ethanol yield of maize stover as measured by a simplified pretreatment and simultaneous saccharification and fermentation assay. Twelve maize varieties selected to be diverse for stover digestibility and composition were evaluated.</p> <p>Results</p> <p>Variation in ethanol yield was driven by glucan convertibility rather than by glucan content. Convertibility was highly correlated with ruminal digestibility and lignin content. There was no relationship between structural carbohydrate content (glucan and neutral detergent fiber) and ethanol yield. However, when these variables were included in multiple regression equations including convertibility or neutral detergent fiber digestibility, their partial regression coefficients were significant and positive. A regression model including both neutral detergent fiber and its ruminal digestibility explained 95% of the variation in ethanol yield.</p> <p>Conclusion</p> <p>Forage quality and composition measurements may be used to predict cellulosic ethanol yield to guide biofeedstock improvement through agronomic research and plant breeding.</p

Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay

<p>Abstract</p> <p>Background</p> <p>The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion process is the ease with which a particular feedstock is hydrolyzed and fermented. Because monitoring the analytes in hydrolysis and fermentation experiments is time consuming, the objective of this study was to develop a rapid fluorescence-based method to monitor sugar production during biomass hydrolysis, and to demonstrate its application in monitoring corn stover hydrolysis.</p> <p>Results</p> <p>Hydrolytic enzymes were used in conjunction with <it>Escherichia coli </it>strain CA8404 (a hexose and pentose-consuming strain), modified to produce green fluorescent protein (GFP). The combination of hydrolytic enzymes and a sugar-consuming organism minimizes feedback inhibition of the hydrolytic enzymes. We observed that culture growth rate as measured by change in culture turbidity is proportional to GFP fluorescence and total growth and growth rate depends upon how much sugar is present at inoculation. Furthermore, it was possible to monitor the course of enzymatic hydrolysis in near real-time, though there are instrumentation challenges in doing this.</p> <p>Conclusion</p> <p>We found that instantaneous fluorescence is proportional to the bacterial growth rate. As growth rate is limited by the availability of sugar, the integral of fluorescence is proportional to the amount of sugar consumed by the microbe. We demonstrate that corn stover varieties can be differentiated based on sugar yields in enzymatic hydrolysis reactions using post-hydrolysis fluorescence measurements. Also, it may be possible to monitor fluorescence in real-time during hydrolysis to compare different hydrolysis protocols.</p

Onderzoek naar de energetische waarde van monsters saucijzebroodjes en boterhamworst

In februari 1988 werden 26 monsters saucijzebroodjes en 27 monsters boterhamworst onderzocht op de gehalten aan vocht en vet. Met behulp van deze gehalten werden de energetische waarden van de monsters berekend. De gemiddelde energetische waarde van de monsters saucijzebroodjes was 1622 kJ/100 g, die van boterhamworst 1266 kJ/100 g. Vergelijking van de waarden van de boterhamworst met die van een in 1982 uitgevoerd onderzoek leert dat de gemiddelde energetische waarde van boterhamworst sinds 1982 met 12,5% is gedaald

Simplicial Complex based Point Correspondence between Images warped onto Manifolds

Recent increase in the availability of warped images projected onto a manifold (e.g., omnidirectional spherical images), coupled with the success of higher-order assignment methods, has sparked an interest in the search for improved higher-order matching algorithms on warped images due to projection. Although currently, several existing methods "flatten" such 3D images to use planar graph / hypergraph matching methods, they still suffer from severe distortions and other undesired artifacts, which result in inaccurate matching. Alternatively, current planar methods cannot be trivially extended to effectively match points on images warped onto manifolds. Hence, matching on these warped images persists as a formidable challenge. In this paper, we pose the assignment problem as finding a bijective map between two graph induced simplicial complexes, which are higher-order analogues of graphs. We propose a constrained quadratic assignment problem (QAP) that matches each p-skeleton of the simplicial complexes, iterating from the highest to the lowest dimension. The accuracy and robustness of our approach are illustrated on both synthetic and real-world spherical / warped (projected) images with known ground-truth correspondences. We significantly outperform existing state-of-the-art spherical matching methods on a diverse set of datasets.Comment: Accepted at ECCV 202

The effect of compressive strain on the Raman modes of the dry and hydrated BaCe0.8Y0.2O3 proton conductor

The BaCe0.8Y0.2O3-{\delta} proton conductor under hydration and under compressive strain has been analyzed with high pressure Raman spectroscopy and high pressure x-ray diffraction. The pressure dependent variation of the Ag and B2g bending modes from the O-Ce-O unit is suppressed when the proton conductor is hydrated, affecting directly the proton transfer by locally changing the electron density of the oxygen ions. Compressive strain causes a hardening of the Ce-O stretching bond. The activation barrier for proton conductivity is raised, in line with recent findings using high pressure and high temperature impedance spectroscopy. The increasing Raman frequency of the B1g and B3g modes thus implies that the phonons become hardened and increase the vibration energy in the a-c crystal plane upon compressive strain, whereas phonons are relaxed in the b-axis, and thus reveal softening of the Ag and B2g modes. Lattice toughening in the a-c crystal plane raises therefore a higher activation barrier for proton transfer and thus anisotropic conductivity. The experimental findings of the interaction of protons with the ceramic host lattice under external strain may provide a general guideline for yet to develop epitaxial strained proton conducting thin film systems with high proton mobility and low activation energy

Image rights: Exploitation and legal control in English and Hungarian law

In the past decades due to changed technical advances, features of the personality have become economically exploitable to an extent not previously known. Pop stars, TV celebrities as well as famous athletes have sought protection against the commercial use of their images, names and likenesses without their consent.1 Despite the economic value of personality and image rights, there is currently no international standard or agreed legal concept for recognising an image right. While many jurisdictions, for example, the US, Germany, France and Hungary offer express statutory protection against the unauthorised commercial use of an individual’s image by a third party in the context of publicity or personality rights, English law provides no cause of action for the infringement of image rights as such. Although a celebrity may currently obtain protection through various statutory and common law rights, such as the developing law of privacy, trade mark law breach of confidence and, in particular, the tort of passing off, none of these rights were designed to protect image or personality rights.2 In this context, this article explores the potentially enforceable rights, their benefits and practical strategies to protect name and image rights in the UK3 and Hungary