8 research outputs found
“Click” Immobilization of a VEGF-Mimetic Peptide on Decellularized Endothelial Extracellular Matrix to Enhance Angiogenesis
We show that coating of decellularized
extracellular matrix (DC-ECM)
on substrate surfaces is an efficient way to generate a platform mimicking
the native ECM environment. Moreover, the DC-ECM can be modified with
a peptide (QK) mimicking vascular endothelial growth factor without
apparently compromising its integrity. The modification was achieved
through metabolic incorporation of a “clickable” handle
to DC-ECM followed by rapid attachment of the QK peptide with an azido
tag using copper-catalyzed click reaction. The attachment of the QK
peptide on to DC-ECM in this way further enhanced the angiogenic responses
(formation of branched tubular networks) of endothelial cells