899 research outputs found

    An Integrative Healthcare Approach to Empowering Older Adults in Creating Advance Directives

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    The purpose of this study is to determine the best practice strategies for creating advance directives when using an integrated team approach. Healthcare professionals are required to inform nursing home residents of their rights around advance directives. Healthcare professionals are also called to assist nursing home residents in creating an advance directive, without coercion, so that it reflects their values around death and dying. Six healthcare professionals (consisting of four social workers, one nurse, and one chaplain) were interviewed to determine their beliefs about the integrative team approach to creating advance directives. The qualitative interviews were analyzed from a grounded theory approach. The ecological perspective for healthcare social workers was used to further conceptualize the data. This study found that having early and frequent discussions with the patient and their family was essential to creating an advance directive. Finding healthcare professionals who are confident and comfortable with talking about death and dying is also beneficial in advance directive discussions. Having an agency that values holistic approaches to healthcare equated to valuing integrative team approaches when discussing advance directives. This study concludes that implementing advance directive strategies with integrative team work remains an abstract theory that lacks evidence of use between these two approaches. Based on the responses around strategies to create advance directives and how integrated teams work together it would appear that the integrative approach to creating advance directives would be successful in accurately documenting the patient’s values and wishes around death and dying

    Factors controlling the lipid composition of marine planktonic Thaumarchaeota

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    Marine ammonia-oxidizing archaea of the phylum Thaumarchaeota synthesize glycerol dibiphytanyl glycerol tetraether (GDGT) membrane lipids that are used as for the reconstruction of sea surface temperatures by means of the TEX86 paleothermometer. Discrepancies between observed and predicted TEX86-temperatures throughout the global ocean indicate that the physiological and ecological controls on lipid composition in planktonic Thaumarchaeota remain poorly understood. In this thesis, the influence of growth phase, temperature, pH, and salinity on the membrane lipid composition of Thaumarchaeota were investigated. The results indicate that TEX86 is not only influenced by temperature but similarly by growth phase and community composition, which compromises its utility as a paleothermometer. A comprehensive study of the lipidomes of 11 thaumarchaeal cultures revealed that Thaumarchaeota contain a high diversity of membrane lipids that is related to phylogeny as well as growth characteristics. This analysis further revealed that all Thaumarchaeota contain the specific biomarkers crenarchaeol, methoxy archaeol, and saturated menaquinone-6

    Performance of Kentucky bluegrass varieties

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    1 online resource (PDF, 2 pages)This archival publication may not reflect current scientific knowledge or recommendations. Current information available from the University of Minnesota Extension: https://www.extension.umn.edu

    On admissibility criteria for weak solutions of the Euler equations

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    We consider solutions to the Cauchy problem for the incompressible Euler equations satisfying several additional requirements, like the global and local energy inequalities. Using some techniques introduced in an earlier paper we show that, for some bounded compactly supported initial data, none of these admissibility criteria singles out a unique weak solution. As a byproduct we show bounded initial data for which admissible solutions to the p-system of isentropic gas dynamics in Eulerian coordinates are not unique in more than one space dimension.Comment: 33 pages, 1 figure; v2: 35 pages, corrected typos, clarified proof

    Unusual butane- and pentanetriol-based tetraether lipids in Methanomassiliicoccus luminyensis, a representative of the seventh order of methanogens

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    Author Posting. © The Author(s), 2016. This is the author's version of the work. It is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 82 (2016): 4505-4516, doi:10.1128/AEM.00772-16.A new clade of archaea has recently been proposed to constitute the seventh methanogenic order, the Methanomassiliicoccales, which is related to the Thermoplasmatales and the uncultivated archaeal clades deep-sea hydrothermal vent Euryarchaeota group 2 and marine group II Euryarchaeota but only distantly related to other methanogens. In this study, we investigated the membrane lipid composition of Methanomassiliicoccus luminyensis, the sole cultured representative of this seventh order. The lipid inventory of M. luminyensis comprises a unique assemblage of novel lipids as well as lipids otherwise typical for thermophilic, methanogenic, or halophilic archaea. For instance, glycerol sesterpanyl-phytanyl diether core lipids found mainly in halophilic archaea were detected, and so were compounds bearing either heptose or methoxylated glycosidic head groups, neither of which have been reported so far for other archaea. The absence of quinones or methanophenazines is consistent with a biochemistry of methanogenesis different from that of the methanophenazine-containing methylotrophic methanogens. The most distinctive characteristic of the membrane lipid composition of M. luminyensis, however, is the presence of tetraether lipids in which one glycerol backbone is replaced by either butane- or pentanetriol, i.e., lipids recently discovered in marine sediments. Butanetriol dibiphytanyl glycerol tetraether (BDGT) constitutes the most abundant core lipid type (>50% relative abundance) in M. luminyensis. We have thus identified a source for these unusual orphan lipids. The complementary analysis of diverse marine sediment samples showed that BDGTs are widespread in anoxic layers, suggesting an environmental significance of Methanomassiliicoccales and/or related BDGT producers beyond gastrointestinal tracts.This study was funded by the European Research Council under the European Union's Seventh Framework Programme “Ideas” Specific Programme, ERC grant agreement no. 247153 (Advanced Grant DARCLIFE; principal investigator, K.-U.H.), and by the Deutsche Forschungsgemeinschaft through the Gottfried Wilhelm Leibniz Prize awarded to K.-U.H. (Hi 616-14-1) and instrument grant Inst 144/300-1 (LC-qToF system). A.S. and T.U. were financially supported by a UNI:DOCS fellowship and a short-term travel grant (KWA) from the University of Vienna (Austria).2016-11-1

    Multiple environmental parameters impact lipid cyclization in Sulfolobus acidocaldarius

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    Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162708/2/emi15194.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162708/1/emi15194_am.pd

    From cars to bikes : the feasibility and effect of using e-bikes, longtail bikes and traditional bikes for transportation among parents of children attending kindergarten : design of a randomized cross-over trial

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    Background: The present study aims to increase bicycling and level of physical activity (PA), and thereby promote health in parents of toddlers, by giving access to different bicycle types. There is a need for greater understanding of e-bikes and their role in the transportation network, and further effects on PA levels and health. Moreover, longtail bikes could meet certain practical needs not fulfilled by e-bikes or traditional bikes, hence increased knowledge regarding their feasibility should be obtained. No previous studies have investigated whether providing an e-bike or a longtail bike over an extended period in a sample of parents of toddlers influence objectively assessed amount of bicycling and total PA level, transportation habits, cardiorespiratory fitness, body composition and blood pressure. Methods: A randomized cross-over trial will be performed, entailing that participants in the intervention group (n = 18) complete the following intervention arms in random order: (i) three months access to an e-bicycle with trailer for child transportation (n = 6), (ii) three months access to a longtail bicycle (n = 6), and (iii) three months access to a regular bicycle with trailer (n = 6), in total nine months. Also, a control group (n = 18) maintaining usual transportation and PA habits will be included. A convenience sample consisting of 36 parents of toddlers residing in Kristians and municipality, Southern Norway, will be recruited. Total amount of bicycling (distance and time), total level of PA, and transportation habits will be measured at baseline and in connection to each intervention arm. Cardiorespiratory fitness, body composition and blood pressure will be measured at baseline and post-intervention. Main outcome will be bicycling distance and time spent cycling. Discussion: New knowledge relevant for the timely issues of public health and environmental sustainability will be provided among parents of toddlers, representing a target group of greatest importance. There is a call for research on the influence of e-bikes and longtail bikes on travel behavior and PA levels, and whether voluntary cycling could improve health. If the present study reveals promising results, it should be replicated in larger and more representative samples. Eventually, inclusion in national public health policies should be considered

    CO_2-dependent carbon isotope fractionation in Archaea, Part I: Modeling the 3HP/4HB pathway

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    The 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) pathway of carbon fixation is found in thermophilic Crenarchaeota of the order Sulfolobales and in aerobic, ammonia-oxidizing Thaumarchaeota. Unlike all other known autotrophic carbon metabolisms, this pathway exclusively uses HCO_3- rather than CO_2 as the substrate for carbon fixation. Biomass produced by the 3HB/4HP pathway is relatively ^(13)C-enriched compared to biomass fixed by other autotrophic pathways, with total biosynthetic isotope effects (ε_(Ar)) of ca. 3‰ in the Sulfolobales and ca. 20‰ in the Thaumarchaeota. Explanations for the difference between these values usually invoke the dual effects of thermophily and growth at low pH (low [HCO_3-]) for the former group vs. mesophily and growth at pH > 7 (high [HCO_3-]) for the latter group. Here we examine the model taxa Metallosphaera sedula and Nitrosopumilus maritimususing an isotope flux-balance model to argue that the primary cause of different ε_(Ar) values more likely is the presence of carbonic anhydrase in M. sedula and its corresponding absence in N. maritimus. The results suggest that the pool of HCO_3-inside N. maritimus is out of isotopic equilibrium with CO_2 and that the organism imports < 10% HCO_3- from the extracellular environment. If correct and generalizable, the aerobic, ammonia-oxidizing marine Thaumarchaeota are dependent on passive CO_2 uptake and a slow rate of intracellular conversion to HCO_3-. Values of ε_(Ar) should therefore vary in response to growth rate (μ) and CO_2 availability, analogous to eukaryotic algae, but in the opposite direction: ε_(Ar) becomes smaller as [CO_(2(aq))] increases and/or μ decreases. Such an idea represents a testable hypothesis, both in the laboratory and in natural systems. Sensitivity to μ and CO_2 implies that measurements of ε_(Ar) may hold promise as a pCO_2 paleobarometer

    PROCESIÓN DEL PENDÓN DE LA CONQUISTA EN SANTA ANA [Material gráfico]

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    Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201
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