Concept Validation for Selective Heating and Press Hardening of Automotive Safety Components with Tailored Properties

© (2014) Trans Tech Publications, Switzerland.A new strategy termed selective heating and press hardening, for hot stamping of boron steel parts with tailored properties is proposed in this paper. Feasibility studies were carried out through a specially designed experimental programme. The main aim was to validate the strategy and demonstrate its potential for structural optimisation. In the work, a lab-scale demonstrator part was designed, and relevant manufacturing and property-assessment processes were defined. A heating technique and selective-heating rigs were designed to enable certain microstructural distributions in blanks to be obtained. A hot stamping tool set was designed for forming and quenching the parts. Demonstrator parts of full martensite phase, full initial phase, and differentially graded microstructures have been formed with high dimensional quality. Hardness testing and three point bending tests were conducted to assess the microstructure distribution and load bearing performance of the as-formed parts, respectively. The feasibility of the concept has been validated by the testing results

Cholecystectomy in patients with coronary heart disease

No Abstrac

Fast and Efficient Discrimination of Traveling Salesperson Problem Stimulus Difficulty.

The Travelling Salesperson Problem (TSP) is a computationally difficult combinatorial optimization problem. In spite of its relative difficulty human solvers are able to generate close-to-optimal solutions in a close-to-linear time frame, and it has been suggested that this is due to the visual system’s inherent sensitivity to certain geometric properties of TSP stimuli. In the current study we employed a novel experimental paradigm in which we presented participants with sets of four TSP stimuli that varied in terms of their relative solution difficulty and asked them to indicate which of the four stimuli they would prefer to solve. The results indicated that the participants’ choice frequencies followed the same ordering as the stimuli’s empirical solution difficulty; i.e., easy-to-solve stimuli were chosen with a higher frequency than hard-to-solve stimuli. It is suggested that these results provide further evidence of the speed and efficiency of human processing of TSPs, and provide further evidence implicating the role of rapid visuo-perceptual organization in generating TSP solutions. An analysis of the geometric properties of the stimuli uncovered a number of factors that may have influenced the choice preferences of participants in the current experiment, and the performance quality of participants in previous experiments

Influence of genome-scale RNA structure disruption on the replication of murine norovirus--similar replication kinetics in cell culture but attenuation of viral fitness in vivo

Mechanisms by which certain RNA viruses, such as hepatitis C virus, establish persistent infections and cause chronic disease are of fundamental importance in viral pathogenesis. Mammalian positive-stranded RNA viruses establishing persistence typically possess genome-scale ordered RNA secondary structure (GORS) in their genomes. Murine norovirus (MNV) persists in immunocompetent mice and provides an experimental model to functionally characterize GORS. Substitution mutants were constructed with coding sequences in NS3/4- and NS6/7-coding regions replaced with sequences with identical coding and (di-)nucleotide composition but disrupted RNA secondary structure (F1, F2, F1/F2 mutants). Mutants replicated with similar kinetics to wild-type (WT) MNV3 in RAW264.7 cells and primary macrophages, exhibited similar (highly restricted) induction and susceptibility to interferon-coupled cellular responses and equal replication fitness by serial passaging of co-cultures. In vivo, both WT and F1/F2 mutant viruses persistently infected mice, although F1, F2 and F1/F2 mutant viruses were rapidly eliminated 1–7 days post-inoculation in competition experiments with WT. F1/F2 mutants recovered from tissues at 9 months showed higher synonymous substitution rates than WT and nucleotide substitutions that potentially restored of RNA secondary structure. GORS plays no role in basic replication of MNV but potentially contributes to viral fitness and persistence in vivo

Letter to Andrew Inglis Clark, Tasmania from Jeffrey Dry, London, 15 Mar 1905

Letter to Andrew Inglis Clark, Tasmania from Jeffrey Dry, London, 15 Mar 1905 regarding British politics. C4/C53

VarDict: a novel and versatile variant caller for next-generation sequencing in cancer research

Accurate variant calling in next generation sequencing (NGS) is critical to understand cancer genomes better. Here we present VarDict, a novel and versatile variant caller for both DNA- and RNA-sequencing data. VarDict simultaneously calls SNV, MNV, InDels, complex and structural variants, expanding the detected genetic driver landscape of tumors. It performs local realignments on the fly for more accurate allele frequency estimation. VarDict performance scales linearly to sequencing depth, enabling ultra-deep sequencing used to explore tumor evolution or detect tumor DNA circulating in blood. In addition, VarDict performs amplicon aware variant calling for polymerase chain reaction (PCR)-based targeted sequencing often used in diagnostic settings, and is able to detect PCR artifacts. Finally, VarDict also detects differences in somatic and loss of heterozygosity variants between paired samples. VarDict reprocessing of The Cancer Genome Atlas (TCGA) Lung Adenocarcinoma dataset called known driver mutations in KRAS, EGFR, BRAF, PIK3CA and MET in 16% more patients than previously published variant calls. We believe VarDict will greatly facilitate application of NGS in clinical cancer research