Development of a temperature-compensated hot-film anemometer system for boundary-layer transition detection on high-performance aircraft

A hot-film constant-temperature anemometer (CTA) system was flight-tested and evaluated as a candidate sensor for determining boundary-layer transition on high-performance aircraft. The hot-film gage withstood an extreme flow environment characterized by shock waves and high dynamic pressures, although sensitivity to the local total temperature with the CTA indicated the need for some form of temperature compensation. A temperature-compensation scheme was developed and two CTAs were modified and flight-tested on the F-104/Flight Test Fixture (FTF) facility at a variety of Mach numbers and altitudes, ranging from 0.4 to 1.8 and 5,000 to 40,000 ft respectively

Wireless, in-vessel neutron monitor for initial core-loading of advanced breeder reactors

An experimental wireless, in-vessel neutron monitor was developed to measure the reactivity of an advanced breeder reactor as the core is loaded for the first time to preclude an accidental critically incident. The environment is liquid sodium at a temperature of approx. 220 C, with negligible gamma or neutron radiation. With ultrasonic transmission of neutron data, no fundamental limitation was observed after tests at 230 C for 2000 h. The neutron sensitivity was approx. 1 count/s-nv, and the potential data transmission rate was approx. 10,000 counts/s

Differential effects of energy stress on AMPK phosphorylation and apoptosis in experimental brain tumor and normal brain

<p>Abstract</p> <p>Background</p> <p>AMP-activated protein kinase (AMPK) is a known physiological cellular energy sensor and becomes phosphorylated at Thr-172 in response to changes in cellular ATP levels. Activated AMPK acts as either an inducer or suppressor of apoptosis depending on the severity of energy stress and the presence or absence of certain functional tumor suppressor genes.</p> <p>Results</p> <p>Here we show that energy stress differentially affects AMPK phosphorylation and cell-death in brain tumor tissue and in tissue from contra-lateral normal brain. We compared TSC2 deficient CT-2A mouse astrocytoma cells with syngeneic normal astrocytes that were grown under identical condition <it>in vitro</it>. Energy stress induced by glucose withdrawal or addition of 2-deoxyglucose caused more ATP depletion, AMPK phosphorylation and apoptosis in CT-2A cells than in the normal astrocytes. Under normal energy conditions pharmacological stimulation of AMPK caused apoptosis in CT-2A cells but not in astrocytes. TSC2 siRNA treated astrocytes are hypersensitive to apoptosis induced by energy stress compared to control cells. AMPK phosphorylation and apoptosis were also greater in the CT-2A tumor tissue than in the normal brain tissue following implementation of dietary energy restriction. Inefficient mTOR and TSC2 signaling, downstream of AMPK, is responsible for CT-2A cell-death, while functional LKB1 may protect normal brain cells under energy stress.</p> <p>Conclusion</p> <p>Together these data demonstrates that AMPK phosphorylation induces apoptosis in mouse astrocytoma but may protect normal brain cells from apoptosis under similar energy stress condition. Therefore, using activator of AMPK along with glycolysis inhibitor could be a potential therapeutic approach for TSC2 deficient human malignant astrocytoma.</p

PHIDL: Python CAD layout and geometry creation for nanolithography

Computer-aided design (CAD) has become a critical element in the creation of nanopatterned structures and devices. In particular, with the increased adoption of easy-to-learn programming languages like Python there has been a significant rise in the amount of lithographic geometries generated through scripting and programming. However, there are currently unaddressed gaps in usability for open-source CAD tools -- especially those in the GDSII design space -- that prevent wider adoption by scientists and students who might otherwise benefit from scripted design. For example, constructing relations between adjacent geometries is often much more difficult than necessary -- spacing a resonator structure a few micrometers from a readout structure often requires manually-coding the placement arithmetic. While inconveniences like this can be overcome by writing custom functions, they are often significant barriers to entry for new users or those less familiar with programming. To help streamline the design process and reduce barrier to entry for scripting designs, we have developed PHIDL, an open-source GDSII-based CAD tool for Python 2 and 3

Resident Gut Microbiota Mediates Exercise Capacity and Tissue Metabolomes in Mice

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