41 research outputs found
Relating the composition and interface interactions in the hard corona of gold nanoparticles to the induced response mechanisms in living cells
Plant protein phosphorylation monitored by capillary liquid chromatography-element mass spectrometry
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Recycling and Separation of Homogeneous Catalyst from Aqueous Multicomponent Mixture by Organic Solvent Nanofiltration
Organic solvent nanofiltration (OSN) has evolved to an established recycling method for homogeneous catalysts. However, commercial availability has not circumvented the need for classification and the scoping of possible applications for specific solvent mixtures. Therefore, Evonik’s DuraMem® 300 was assessed for the recycling of magnesium triflate at two transmembrane pressures from a mixture of ethanol, ethyl acetate and water. Catalyst retention up to 98% and permeability of up to 4.44·10−1∙L∙bar−1∙m−2∙h−1 were possible when less than 25% ethyl acetate was in the mixture. The retention of some of the components in the ternary mixture was observed while others were enriched, making the membrane also suitable for fractioning thereof
Soziale und biografische Risiken benachteiligter Jugendlicher: Das Zusammenspiel der Erziehungsaufträge von Jugendhilfe und Schule
Mass spectrometric approach for the analysis of the hard protein corona of nanoparticles in living cells
Quantitative Evaluation of Cisplatin Uptake in Sensitive and Resistant Individual Cells by Single-Cell ICP-MS (SC-ICP-MS)
One
of the main limitations to the Pt-therapy in cancer is the
development of associated drug resistance that can be associated with
a significant reduction of the intracellular platinum concentration.
Thus, intracellular Pt concentration could be considered as a biomarker
of cisplatin resistance. In this work, an alternative method to address
intracellular Pt concentration in individual cells is explored to
permit the evaluation of different cell models and alternative therapies
in a relatively fast way. For this aim, total Pt analysis in single
cells has been implemented using a total consumption nebulizer coupled
to inductively coupled plasma mass spectrometric detection (ICP-MS).
The efficiency of the proposed device has been evaluated in combination
with flow cytometry and turned out to be around 25% (cells entering
the ICP-MS from the cells in suspension). Quantitative uptake studies
of a nontoxic Tb-containing compound by individual cells were conducted
and the results compared to those obtained by bulk analysis of the
same cells. Both sets of data were statistically comparable. Thus,
final application of the developed methodology to the comparative
uptake of Pt-species in cisplatin resistant and sensitive cell lines
(A2780cis and A2780) was conducted. The results obtained revealed
the potential of this analytical strategy to differentiate between
different cell lines of different sensitivity to the drug which might
be of high medical interest