Properties of Dust Grains in Planetary Nebulae -- I. The Ionized Region of NGC 6445

In this paper we study new infrared spectra of the evolved planetary nebula NGC 6445 obtained with ISO. These data show that the thermal emission from the grains is very cool and has a low flux compared to H beta. A model of the ionized region is constructed, using the photo-ionization code CLOUDY 90.05. Based on this model, we show from depletions in the gas phase elements that little grain destruction can have occurred in the ionized region of NGC 6445. We also argue that dust-gas separation in the nebula is not plausible. The most likely conclusion is that grains are residing inside the ionized region of NGC 6445 and that the low temperature and flux of the grain emission are caused by the low luminosity of the central star and the low optical depth of the grains. This implies that the bulk of the silicon-bearing grains in this nebula were able to survive exposure to hard UV photons for at least several thousands of years, contradicting previously published results. A comparison between optical and infrared diagnostic line ratios gives a marginal indication for the presence of a t^2-effect in the nebula. However, the evidence is not convincing and other explanations for the discrepancy are also plausible. The off-source spectrum taken with ISO-LWS clearly shows the presence of a warm cirrus component with a temperature of 24 K as well as a very cold component with a temperature of 7 K. Since our observation encompasses only a small region of the sky, it is not clear how extended the 7 K component is and whether it contributed significantly to the FIRAS spectrum taken by COBE. Because our line of sight is in the galactic plane, the very cold component could be a starless core.Comment: 36 pages, 8 tables, 7 figures, accepted for publication in Ap

The ISO-SWS spectrum of planetary nebula NGC 7027

We present the infrared spectrum of the planetary nebula NGC7027 observed with the Short Wavelength Spectrometer (SWS), on board the Infrared Space Observatory (ISO). These data allow us to derive the electron density and, together with the IUE and optical spectra, the electron temperature for several ions. The nebular composition has: been determined, the evolutionary status of the central star is discussed and the element depletion in the nebula is given. We conclude that the progenitor was: a C-rich star with a mass: between 3 and 4 M-circle dot

Infrared High-Resolution Spectroscopy of Post-AGB Circumstellar Disks. I. HR 4049 - The Winnowing Flow Observed?

High-resolution infrared spectroscopy in the 2.3-4.6 micron region is reported for the peculiar A supergiant, single-lined spectroscopic binary HR 4049. Lines from the CO fundamental and first overtone, OH fundamental, and several H2O vibration-rotation transitions have been observed in the near-infrared spectrum. The spectrum of HR 4049 appears principally in emission through the 3 and 4.6 micron region and in absorption in the 2 micron region. The 4.6 micron spectrum shows a rich 'forest' of emission lines. All the spectral lines observed in the 2.3-4.6 micron spectrum are shown to be circumbinary in origin. The presence of OH and H2O lines confirm the oxygen-rich nature of the circumbinary gas which is in contrast to the previously detected carbon-rich material. The emission and absorption line profiles show that the circumbinary gas is located in a thin, rotating layer near the dust disk. The properties of the dust and gas circumbinary disk and the spectroscopic orbit yield masses for the individual stars, M_AI~0.58 Msolar and M_MV~0.34 Msolar. Gas in the disk also has an outward flow with a velocity of $\gtrsim$ 1 km/s. The severe depletion of refractory elements but near-solar abundances of volatile elements observed in HR 4049 results from abundance winnowing. The separation of the volatiles from the grains in the disk and the subsequent accretion by the star are discussed. Contrary to prior reports, the HR 4049 carbon and oxygen isotopic abundances are typical AGB values: 12C/13C=6^{+9}_{-4} and 16O/17O>200.Comment: 42 pages, 14 figures, Accepted by Ap

The Evolution of NGC 7027 at Radio Frequencies: A New Determination of the Distance and Core Mass

We present the results of a 25-year program to monitor the radio flux evolution of the planetary nebula NGC7027. We find significant evolution of the spectral flux densities. The flux density at 1465 MHz, where the nebula is optically thick, is increasing at a rate of 0.251+-0.015 % per year, caused by the expansion of the ionized nebula. At frequencies where the emission is optically thin, the spectral flux density is changing at a rate of -0.145+-0.005 % per year, caused by a decrease in the number of ionizing photons coming from the central star. A distance of 980+-100 pc is derived. By fitting interpolated models of post-AGB evolution to the observed changes, we find that over the 25-yr monitoring period, the stellar temperature has increased by 3900+-900 K and the stellar bolometric luminosity has decreased by 1.75+-0.38 %. We derive a distance-independent stellar mass of 0.655+-0.01 solar masses adopting the Bloecker stellar evolution models, or about 0.04 solar masses higher when using models of Vassiliadis & Wood which may provide a better fit. A Cloudy photoionization model is used to fit all epochs at all frequencies simultaneously. The differences between the radio flux density predictions and the observed values show some time-independent residuals of typically 1 %. A possible explanation is inaccuracies in the radio flux scale of Baars et al. We propose an adjustment to the flux density scale of the primary radio flux calibrator 3C286, based on the Cloudy model of NGC7027. We also calculate precise flux densities for NGC7027 for all standard continuum bands used at the VLA, as well as for some new 30GHz experiments.Comment: submitted to the Astrophysical Journa

Brassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria

Brassica juncea BjCHI1 is a plant chitinase with two chitin-binding domains. Its expression, induced in response to wounding, methyl jasmonate treatment, Aspergillus niger infection, and caterpillar Pieris rapae feeding, suggests that it plays a role in defence. In this study, to investigate the potential of using BjCHI1 in agriculture, Pichia-expressed BjCHI1 and its deletion derivatives that lack one or both chitin-binding domains were tested against phytopathogenic fungi and bacteria. Transplastomic tobacco expressing BjCHI1 was also generated and its extracts assessed. In radial growth-inhibition assays, BjCHI1 and its derivative with one chitin-binding domain showed anti-fungal activities against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea, and Ascochyta rabiei. BjCHI1 also inhibited spore germination of C. truncatum. Furthermore, BjCHI1, but not its derivatives lacking one or both domains, inhibited the growth of Gram-negative bacteria (Escherichia coli, Ralstonia solanacearum, Pseudomonas aeruginosa) more effectively than Gram-positive bacteria (Micrococcus luteus and Bacillus megaterium), indicating that the duplicated chitin-binding domain, uncommon in chitinases, is essential for bacterial agglutination. Galactose, glucose, and lactose relieved agglutination, suggesting that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. Retention of chitinase and bacterial agglutination activities in transplastomic tobacco extracts implicates that BjCHI1 is potentially useful against both fungal and bacterial phytopathogens in agriculture

Black Terns <i>Chlidonias niger</i> and their dietary problems in Dutch wetlands

Black Terns Chlidonias niger have shown a decrease of well over 90% as a breeding bird in The Netherlands during the twentieth century. Two hypotheses have been put forward for this decline: the disappearance of the floating plant Water Soldier Stratiotes aloides, which used to be the favourite nesting substrate of the terns, and a decrease of available insect food for the chicks, notably dragonflies. Both effects are attributed to eutrophication of surface waters. Reproductive bottlenecks vary greatly among areas and habitats. In river landscapes, no signs of food shortage could be found, and loss of nesting substrate has been successfully compensated for by offering artificial nest rafts. Extremely low fledging success in moors and in lowland grasslands is caused by food problems. In this case, artificial rafts are less successful. With decreased insect availability, fish and earthworms have become more important in the chicks' diet, but these are less reliable as a food source. Fledging success greatly depends on the amount of fish in the diet. Also, a minimum amount of fish is always needed to cover the calcium need of the chicks. In north-eastern Poland, there were no problems with either nesting places or food for the chicks.</p