The Changing Role of the School of Agriculture at South Dakota State College

In 1908, at the time of the origin of the School of Agriculture at South Dakota State College, the sponsors recognized that there existed a real need for a school of vocational agriculture and home economics. Hundreds of rural young people were leaving school upon the completion of the elementary grades, or at best a year or two of high school, and permanently entering into their occupation on the farms of the state. Agricultural and home economics skills were transferred to the younger generation largely by apprenticeship methods

ENHANCED GRAVITROPISM 2 coordinates molecular adaptations to gravistimulation in the elongation zone of barley roots

Root gravitropism includes gravity perception in the root cap, signal transduction between root cap and elongation zone, and curvature response in the elongation zone. The barley (Hordeum vulgare) mutant enhanced gravitropism 2 (egt2) displays a hypergravitropic root phenotype. We compared the transcriptomic reprogramming of the root cap, the meristem, and the elongation zone of wild-type (WT) and egt2 seminal roots upon gravistimulation in a time-course experiment and identified direct interaction partners of EGT2 by yeast-two-hybrid screening and bimolecular fluorescence complementation validation. We demonstrated that the elongation zone is subjected to most transcriptomic changes after gravistimulation. Here, 33% of graviregulated genes are also transcriptionally controlled by EGT2, suggesting a central role of this gene in controlling the molecular networks associated with gravitropic bending. Gene co-expression analyses suggested a role of EGT2 in cell wall and reactive oxygen species-related processes, in which direct interaction partners of EGT2 regulated by EGT2 and gravity might be involved. Taken together, this study demonstrated the central role of EGT2 and its interaction partners in the networks controlling root zone-specific transcriptomic reprogramming of barley roots upon gravistimulation. These findings can contribute to the development of novel root idiotypes leading to improved crop performance

Isolated chondrons: a viable alternative for studies of chondrocyte metabolism in vitro

OBJECTIVE: To develop and test a simple enzymatic procedure for isolating chondrons, which consist of the chondrocytes and their surrounding pericellular microenvironment. DESIGN: Chondrons were obtained by digesting adult human articular cartilage with a mixture of dispase and collagenase. Chondrons and chondrocytes were cultured in alginate beads, immunofluorescence labeled and examined by confocal microscopy. RESULTS: Comparison of freshly isolated chondrons with cryostat sections of cartilage revealed that type VI collagen, type II collagen and aggrecan were retained, but fibronectin and a unique chondroitin sulfate epitope recognized by the antibody, 7D4, were lost. Comparison of enzymatic and mechanical homogenization methods revealed subtle changes in chondron morphology and retention of fibronectin in mechanically isolated chondrons. Average yield of enzyme-isolated chondrons was slightly lower than that of chondrocytes isolated by pronase and collagenase digestion, but was much greater than that reported for mechanically isolated chondrons. Enzyme-isolated chondron viability was greater than 80% 1 day after isolation, and continued to be above 80% through 7 weeks of alginate bead culture. Viability of isolated chondrocytes was initially greater than 80% but fell to 60-80% with time in culture. Chondrons and isolated chondrocytes had a similar division rate except osteoarthritic chondrons were significantly slower after 2 weeks in culture. Cell division was more rapid for nonosteoarthritic chondrons than for osteoarthritic ones. CONCLUSIONS: Enzymatic isolation of chondrons is relatively simple, gives better yield and viability than mechanical isolation, but comparable yield and viability of traditional chondrocyte isolation. Enzymatic chondron isolation allows the effect of the in vivo-formed pericellular matrix on chondrocyte metabolism to be studied in vitro

The Concordiensis, Volume 13, Number 2

Editorial Literary: Across the Continent College News: A Banquest to the College; Williams vs. Union; Ridgefield vs. Union; Will Write of Union; A Religious Organization; Union Honored; Football at Elmira; Supreme Court Centennial - Union Men on Committee; The Tennis Tournament; Junior Hop Locals; Personals; Necrologyhttps://digitalworks.union.edu/concordiensis_1889/1007/thumbnail.jp

The Beacon (12/08/1965)

This is a digitized, downloadable version of the University of Rhode Island student newspaper.https://digitalcommons.uri.edu/beacon/1659/thumbnail.jp

Carillon, 1952

https://collected.jcu.edu/carillon/1005/thumbnail.jp

Holland City News, Volume 47, Number 15: April 11, 1918

Newspaper published in Holland, Michigan, from 1872-1977, to serve the English-speaking people in Holland, Michigan. Purchased by local Dutch language newspaper, De Grondwet, owner in 1888.https://digitalcommons.hope.edu/hcn_1918/1014/thumbnail.jp

Mount Vernon Democratic Banner December 6, 1888

The Democratic Banner was a newspaper published weekly in Mount Vernon, Ohio. It later became the Mt Vernon Democratic Banner in December of 1853.https://digital.kenyon.edu/banner1888/1023/thumbnail.jp

Mount Vernon Democratic Banner August 18, 1887

Mount Vernon Democratic Banner was a newspaper published weekly in Mount Vernon, Ohio. Until 1853, it was published as the Democratic Banner.https://digital.kenyon.edu/banner1887/1006/thumbnail.jp

Mount Vernon Democratic Banner May 24, 1888

Mount Vernon Democratic Banner was a newspaper published weekly in Mount Vernon, Ohio. Until 1853, it was published as the Democratic Banner.https://digital.kenyon.edu/banner1888/1012/thumbnail.jp