Use of inedible wheat residues from the KSC-CELSS breadboard facility for production of fungal cellulase

Cellulose and xylan (a hemicellulose) comprise 50 percent of inedible wheat residue (which is 60 percent of total wheat biomass) produced in the Kennedy Space Center Closed Ecological Life Support System (CELSS) Breadboard Biomass Production Chamber (BPC). These polysaccharides can be converted by enzymatic hydrolysis into useful monosaccharides, thus maximizing the use of BPC volume and energy, and minimizing waste material to be treated. The evaluation of CELSS-derived wheat residues for production for cellulase enzyme complex by Trichoderma reesei and supplemental beta-glucosidase by Aspergillus phoenicis is in progress. Results to date are given

Modulation of endothelial cell KCa3.1 Channels during endothelium-derived hyperpolarizing factor signaling in mesenteric resistance arteries

Arterial hyperpolarization to acetylcholine (ACh) reflects coactivation of KCa3.1 (IKCa) channels and KCa2.3 (SKCa) channels in the endothelium that transfers through myoendothelial gap junctions and diffusible factor(s) to affect smooth muscle relaxation (endothelium-derived hyperpolarizing factor [EDHF] response). However, ACh can differentially activate KCa3.1 and KCa2.3 channels, and we investigated the mechanisms responsible in rat mesenteric arteries. KCa3.1 channel input to EDHF hyperpolarization was enhanced by reducing external [Ca2+]o but blocked either with forskolin to activate protein kinase A or by limiting smooth muscle [Ca2+]i increases stimulated by phenylephrine depolarization. Imaging [Ca2+]i within the endothelial cell projections forming myoendothelial gap junctions revealed increases in cytoplasmic [Ca2+]i during endothelial stimulation with ACh that were unaffected by simultaneous increases in muscle [Ca2+]i evoked by phenylephrine. If gap junctions were uncoupled, KCa3.1 channels became the predominant input to EDHF hyperpolarization, and relaxation was inhibited with ouabain, implicating a crucial link through Na+/K+-ATPase. There was no evidence for an equivalent link through KCa2.3 channels nor between these channels and the putative EDHF pathway involving natriuretic peptide receptor-C. Reconstruction of confocal z-stack images from pressurized arteries revealed KCa2.3 immunostain at endothelial cell borders, including endothelial cell projections, whereas KCa3.1 channels and Na+/K+-ATPase {alpha}2/{alpha}3 subunits were highly concentrated in endothelial cell projections and adjacent to myoendothelial gap junctions. Thus, extracellular [Ca2+]o appears to modify KCa3.1 channel activity through a protein kinase A-dependent mechanism independent of changes in endothelial [Ca2+]i. The resulting hyperpolarization links to arterial relaxation largely through Na+/K+-ATPase, possibly reflecting K+ acting as an EDHF. In contrast, KCa2.3 hyperpolarization appears mainly to affect relaxation through myoendothelial gap junctions. Overall, these data suggest that K+ and myoendothelial coupling evoke EDHF-mediated relaxation through distinct, definable pathways

Matching small β\beta functions using centroid jitter and two beam position monitors

Matching to small beta functions is required to preserve emittance in plasma accelerators. The plasma wake provides strong focusing fields, which typically require beta functions on the mm-scale, comparable to those found in the final focusing of a linear collider. Such beams can be time consuming to experimentally produce and diagnose. We present a simple, fast, and noninvasive method to measure Twiss parameters in a linac using two beam position monitors only, relying on the similarity of the beam phase space and the jitter phase space. By benchmarking against conventional quadrupole scans, the viability of this technique was experimentally demonstrated at the FLASHForward plasma-accelerator facility.Comment: 8 pages, 7 figure

Nearby Clumpy, Gas Rich, Star Forming Galaxies: Local Analogs of High Redshift Clumpy Galaxies

Luminous compact blue galaxies (LCBGs) have enhanced star formation rates and compact morphologies. We combine Sloan Digital Sky Survey data with HI data of 29 LCBGs at redshift z~0 to understand their nature. We find that local LCBGs have high atomic gas fractions (~50%) and star formation rates per stellar mass consistent with some high redshift star forming galaxies. Many local LCBGs also have clumpy morphologies, with clumps distributed across their disks. Although rare, these galaxies appear to be similar to the clumpy star forming galaxies commonly observed at z~1-3. Local LCBGs separate into three groups: 1. Interacting galaxies (~20%); 2. Clumpy spirals (~40%); 3. Non-clumpy, non-spirals with regular shapes and smaller effective radii and stellar masses (~40%). It seems that the method of building up a high gas fraction, which then triggers star formation, is not the same for all local LCBGs. This may lead to a dichotomy in galaxy characteristics. We consider possible gas delivery scenarios and suggest that clumpy spirals, preferentially located in clusters and with companions, are smoothly accreting gas from tidally disrupted companions and/or intracluster gas enriched by stripped satellites. Conversely, as non-clumpy galaxies are preferentially located in the field and tend to be isolated, we suggest clumpy, cold streams, which destroy galaxy disks and prevent clump formation, as a likely gas delivery mechanism for these systems. Other possibilities include smooth cold streams, a series of minor mergers, or major interactions.Comment: 22 pages, 5 figure

Indium oxide diffusion barriers for Al/Si metallizations

Indium oxide (In2O3) films were prepared by reactive rf sputtering of an In target in O2/Ar plasma. We have investigated the application of these films as diffusion barriers in Si/In2O3/Al and Si/TiSi2.3/In2O3/Al metallizations. Scanning transmission electron microscopy together with energy dispersive analysis of x ray of cross-sectional Si/In2O3/Al specimens, and electrical measurements on shallow n + -p junction diodes were used to evaluate the diffusion barrier capability of In2O3 films. We find that 100-nm-thick In2O3 layers prevent the intermixing between Al and Si in Si/In2O3/Al contacts up to 650°C for 30 min, which makes this material one of the best thin-film diffusion barriers on record between Al and Si. (The Si-Al eutectic temperature is 577°C, Al melts at 660°C.) When a contacting layer of titanium silicide is incorporated to form a Si/TiSi2.3/In2O3/Al metallization structure, the thermal stability of the contact drops to 600°C for 30 min heat treatment

The Nature of Nearby Counterparts to Intermediate Redshift Luminous Compact Blue Galaxies I. Optical/H I Properties and Dynamical Masses

We present single-dish H I spectra obtained with the Green Bank Telescope, along with optical photometric properties from the Sloan Digital Sky Survey, of 20 nearby (D < 70 Mpc) Luminous Compact Blue Galaxies (LCBGs). These ~L*, blue, high surface brightness, starbursting galaxies were selected with the same criteria used to define LCBGs at higher redshifts. We find these galaxies are gas-rich, with M(HI) ranging from 5*10^8 to 8*10^9 M_sun, and M(HI)/L_B ranging from 0.2 to 2 M_sun/L_sun, consistent with a variety of morphological types of galaxies. We find the dynamical masses (measured within R_25) span a wide range, from 3*10^9 to 1*10^11 M_sun. However, at least half have dynamical mass-to-light ratios smaller than nearby galaxies of all Hubble types, as found for LCBGs at intermediate redshifts. By comparing line widths and effective radii with local galaxy populations, we find that LCBGs are consistent with the dynamical mass properties of Magellanic (low luminosity) spirals, and the more massive irregulars and dwarf ellipticals, such as NGC 205.Comment: 33 pages, 8 figures, accepted by Ap

The Evolution of Luminous Compact Blue Galaxies: Disks or Spheroids?

Luminous compact blue galaxies (LCBGs) are a diverse class of galaxies characterized by high luminosity, blue color, and high surface brightness that sit at the critical juncture of galaxies evolving from the blue to the red sequence. As part of our multi-wavelength survey of local LCBGs, we have been studying the HI content of these galaxies using both single-dish telescopes and interferometers. Our goals are to determine if single-dish HI observations represent a true measure of the dynamical mass of LCBGs and to look for signatures of recent interactions that may be triggering star formation in LCBGs. Our data show that while some LCBGs are undergoing interactions, many appear isolated. While all LCBGs contain HI and show signatures of rotation, the population does not lie on the Tully-Fisher relation nor can it evolve onto it. Furthermore, the HI maps of many LCBGs show signatures of dynamically hot components, suggesting that we are seeing the formation of a thick disk or spheroid in at least some LCBGs. There is good agreement between the HI and H-alpha kinematics for LCBGs, and both are similar in appearance to the H-alpha kinematics of high redshift star-forming galaxies. Our combined data suggest that star formation in LCBGs is primarily quenched by virial heating, consistent with model predictions.Comment: 4 pages, 2 figures, to appear in the proceedings of IAU Symposium 277, "Tracing the Ancestry of Galaxies on the Land of our Ancestors", eds. C. Carignan, K.C. Freeman, and F. Combe