55 research outputs found
The RIP140 Gene Is a Transcriptional Target of E2F1
RIP140 is a transcriptional coregulator involved in energy homeostasis and ovulation which is controlled at the transcriptional level by several nuclear receptors. We demonstrate here that RIP140 is a novel target gene of the E2F1 transcription factor. Bioinformatics analysis, gel shift assay, and chromatin immunoprecipitation demonstrate that the RIP140 promoter contains bona fide E2F response elements. In transiently transfected MCF-7 breast cancer cells, the RIP140 promoter is transactivated by overexpression of E2F1/DP1. Interestingly, RIP140 mRNA is finely regulated during cell cycle progression (5-fold increase at the G1/S and G2/M transitions). The positive regulation by E2F1 requires sequences located in the proximal region of the promoter (−73/+167), involves Sp1 transcription factors, and undergoes a negative feedback control by RIP140. Finally, we show that E2F1 participates in the induction of RIP140 expression during adipocyte differentiation. Altogether, this work identifies the RIP140 gene as a new transcriptional target of E2F1 which may explain some of the effect of E2F1 in both cancer and metabolic diseases
Separation of a New Antidiabetic Agent, N-(Trans-4-Isopropylcyclohexylcarbonyl)-D-Phenylalanine, and its Isomers by Chiral High-Performance Liquid Chromatography
Τενότου Διοπτική (Perspective) / Μεταφρασθείσα μεν εκ του Γαλλικού υπό Πάνου Ν. Πλέσκα γραμματέως της Νομαρχίας των Κυκλάδων, εκδοθείσα δε δαπάνη Μ. Π. Περίδου και Α. Κριεζή.
THE DISPOSITION AND PHARMACOKINETICS OF ALPIDEM, A NEW ANXIOLYTIC, IN THE RAT
ABSTRACT: Th
Metabolism of diethylstilbestrol in the c3h mouse. Chromato- graphic systems for the quantitative analysis of des metabolic products.
Die mikroanalytische, selektive Bestimmung der unveresterten langkettigen Fettsäuren im Serum
Pharmacokinetic profile of a new modified release formulation of zolpidem designed to improve sleep maintenance
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