85 research outputs found
Metastable states of a flux line lattice studied by transport and Small Angle Neutron Scattering
Flux Lines Lattice (FLL) states have been studied using transport
measurements and Small Angle Neutron Scattering in low T materials. In
Pb-In, the bulk dislocations in the FLL do not influence the transport
properties. In Fe doped NbSe, transport properties can differ after a
Field Cooling (FC) or a Zero Field Cooling (ZFC) procedure, as previously
reported. The ZFC FLL is found ordered with narrow Bragg Peaks and is linked to
a linear V(I) curve and to a superficial critical current. The FC FLL pattern
exhibits two Bragg peaks and the corresponding V(I) curve shows a S-shape. This
can be explained by the coexistence of two ordered FLL slightly tilted from the
applied field direction by different superficial currents. These currents are
wiped out when the transport current is increased.Comment: accepted for publication in Phys. Rev.
Freezing of Spinodal Decompostion by Irreversible Chemical Growth Reaction
We present a description of the freezing of spinodal decomposition in
systems, which contain simultaneous irreversible chemical reactions, in the
hydrodynamic limit approximation. From own results we conclude, that the
chemical reaction leads to an onset of spinodal decomposition also in the case
of an initial system which is completely miscible and can lead to an extreme
retardation of the dynamics of the spinodal decomposition, with the probability
of a general freezing of this process, which can be experimetally observed in
simultaneous IPN formation.Comment: 10 page
Why pinning by surface irregularities can explain the peak effect in transport properties and neutron diffraction results in NbSe2 and Bi-2212 crystals?
The existence of a peak effect in transport properties (a maximum of the
critical current as function of magnetic field) is a well-known but still
intriguing feature of type II superconductors such as NbSe2 and Bi-2212. Using
a model of pinning by surface irregularities in anisotropic superconductors, we
have developed a calculation of the critical current which allows estimating
quantitatively the critical current in both the high critical current phase and
in the low critical current phase. The only adjustable parameter of this model
is the angle of the vortices at the surface. The agreement between the
measurements and the model is really very impressive. In this framework, the
anomalous dynamical properties close to the peak effect is due to co-existence
of two different vortex states with different critical currents. Recent neutron
diffraction data in NbSe2 crystals in presence of transport current support
this point of view
Optimal MHC-II-restricted tumor antigen presentation to CD4+ T helper cells: the key issue for development of anti-tumor vaccines
Present immunoprevention and immunotherapeutic approaches against cancer suffer from the limitation of being not “sterilizing” procedures, as very poor protection against the tumor is obtained. Thus newly conceived anti-tumor vaccination strategies are urgently needed. In this review we will focus on ways to provide optimal MHC class II-restricted tumor antigen presentation to CD4+ T helper cells as a crucial parameter to get optimal and protective adaptive immune response against tumor. Through the description of successful preventive or therapeutic experimental approaches to vaccinate the host against the tumor we will show that optimal activation of MHC class II-restricted tumor specific CD4+ T helper cells can be achieved in various ways. Interestingly, the success in tumor eradication and/or growth arrest generated by classical therapies such as radiotherapy and chemotherapy in some instances can be re-interpreted on the basis of an adaptive immune response induced by providing suitable access of tumor-associated antigens to MHC class II molecules. Therefore, focussing on strategies to generate better and suitable MHC class II–restricted activation of tumor specific CD4+ T helper cells may have an important impact on fighting and defeating cancer
Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements
Endogenous retroviral elements (ERVs) in mice are significant genomic mutagens, causing ∼10% of all reported spontaneous germ line mutations in laboratory strains. The majority of these mutations are due to insertions of two high copy ERV families, the IAP and ETn/MusD elements. This significant level of ongoing retrotranspositional activity suggests that inbred mice are highly variable in content of these two ERV groups. However, no comprehensive genome-wide studies have been performed to assess their level of polymorphism. Here we compared three test strains, for which sufficient genomic sequence is available, to each other and to the reference C57BL/6J genome and detected very high levels of insertional polymorphism for both ERV families, with an estimated false discovery rate of only 0.4%. Specifically, we found that at least 60% of IAP and 25% of ETn/MusD elements detected in any strain are absent in one or more of the other three strains. The polymorphic nature of a set of 40 ETn/MusD elements found within gene introns was confirmed using genomic PCR on DNA from a panel of mouse strains. For some cases, we detected gene-splicing abnormalities involving the ERV and obtained additional evidence for decreased gene expression in strains carrying the insertion. In total, we identified nearly 700 polymorphic IAP or ETn/MusD ERVs or solitary LTRs that reside in gene introns, providing potential candidates that may contribute to gene expression differences among strains. These extreme levels of polymorphism suggest that ERV insertions play a significant role in genetic drift of mouse lines
Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements
Endogenous retroviral elements (ERVs) in mice are significant genomic mutagens, causing ∼10% of all reported spontaneous germ line mutations in laboratory strains. The majority of these mutations are due to insertions of two high copy ERV families, the IAP and ETn/MusD elements. This significant level of ongoing retrotranspositional activity suggests that inbred mice are highly variable in content of these two ERV groups. However, no comprehensive genome-wide studies have been performed to assess their level of polymorphism. Here we compared three test strains, for which sufficient genomic sequence is available, to each other and to the reference C57BL/6J genome and detected very high levels of insertional polymorphism for both ERV families, with an estimated false discovery rate of only 0.4%. Specifically, we found that at least 60% of IAP and 25% of ETn/MusD elements detected in any strain are absent in one or more of the other three strains. The polymorphic nature of a set of 40 ETn/MusD elements found within gene introns was confirmed using genomic PCR on DNA from a panel of mouse strains. For some cases, we detected gene-splicing abnormalities involving the ERV and obtained additional evidence for decreased gene expression in strains carrying the insertion. In total, we identified nearly 700 polymorphic IAP or ETn/MusD ERVs or solitary LTRs that reside in gene introns, providing potential candidates that may contribute to gene expression differences among strains. These extreme levels of polymorphism suggest that ERV insertions play a significant role in genetic drift of mouse lines
Retrotransposon-Induced Heterochromatin Spreading in the Mouse Revealed by Insertional Polymorphisms
The “arms race” relationship between transposable elements (TEs) and their host has promoted a series of epigenetic silencing mechanisms directed against TEs. Retrotransposons, a class of TEs, are often located in repressed regions and are thought to induce heterochromatin formation and spreading. However, direct evidence for TE–induced local heterochromatin in mammals is surprisingly scarce. To examine this phenomenon, we chose two mouse embryonic stem (ES) cell lines that possess insertionally polymorphic retrotransposons (IAP, ETn/MusD, and LINE elements) at specific loci in one cell line but not the other. Employing ChIP-seq data for these cell lines, we show that IAP elements robustly induce H3K9me3 and H4K20me3 marks in flanking genomic DNA. In contrast, such heterochromatin is not induced by LINE copies and only by a minority of polymorphic ETn/MusD copies. DNA methylation is independent of the presence of IAP copies, since it is present in flanking regions of both full and empty sites. Finally, such spreading into genes appears to be rare, since the transcriptional start sites of very few genes are less than one Kb from an IAP. However, the B3galtl gene is subject to transcriptional silencing via IAP-induced heterochromatin. Hence, although rare, IAP-induced local heterochromatin spreading into nearby genes may influence expression and, in turn, host fitness
Microscopical study of ancient mortars from Tournai (Belgium)
Masonry mortars and mortars used for flooring (4th-11th century AD) from excavations in the Cathedral at Tournai in Belgium have been investigated as part of a broader study considering the transition of ancient cities from Roman to late Roman society and the transformation to the early middle ages. A selected set of samples has been characterised using a combination of chemical and microscopical techniques. This characterisation enables us to refine our knowledge of the mortar composition, of the original materials and of their provenance. The results of the characterisation of these samples clearly indicate the importance of an optical microscopy study using thin sections as a first step in the chemical-mineralogical characterisation of historic mortars because of the complexity and heterogeneity of this composite material. Microprobe analysis results on lime lumps have clearly proved to be useful to determine the hydraulicity of ancient mortars. (C) 2004 Elsevier Inc. All rights reserved
- …