High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa).

CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable. To provide more insights into the efficiency of creating stable homozygous mutants using CRISPR/Cas9, we targeted LsNCED4 (9-cis-EPOXYCAROTENOID DIOXYGENASE4), a gene conditioning thermoinhibition of seed germination in lettuce. Three constructs, each capable of expressing Cas9 and a single gRNA targeting different sites in LsNCED4, were stably transformed into lettuce (Lactuca sativa) cvs. Salinas and Cobham Green. Analysis of 47 primary transformants (T1) and 368 T2 plants by deep amplicon sequencing revealed that 57% of T1 plants contained events at the target site: 28% of plants had germline mutations in one allele indicative of an early editing event (mono-allelic), 8% of plants had germline mutations in both alleles indicative of two early editing events (bi-allelic), and the remaining 21% of plants had multiple low frequency mutations indicative of late events (chimeric plants). Editing efficiency was similar in both genotypes, while the different gRNAs varied in efficiency. Amplicon sequencing of 20 T1 and more than 100 T2 plants for each of the three gRNAs showed that repair outcomes were not random, but reproducible and characteristic for each gRNA. Knockouts of NCED4 resulted in large increases in the maximum temperature for seed germination, with seeds of both cultivars capable of germinating >70% at 37°. Knockouts of NCED4 provide a whole-plant selectable phenotype that has minimal pleiotropic consequences. Targeting NCED4 in a co-editing strategy could therefore be used to enrich for germline-edited events simply by germinating seeds at high temperature

Assessment of China's virtual air pollution transport embodied in trade by using a consumption-based emission inventory

Substantial anthropogenic emissions from China have resulted in serious air pollution, and this has generated considerable academic and public concern. The physical transport of air pollutants in the atmosphere has been extensively investigated; however, understanding the mechanisms how the pollutant was transferred through economic and trade activities remains a challenge. For the first time, we quantified and tracked China's air pollutant emission flows embodied in interprovincial trade, using a multiregional input - output model framework. Trade relative emissions for four key air pollutants (primary fine particle matter, sulfur dioxide, nitrogen oxides and non-methane volatile organic compounds) were assessed for 2007 in each Chinese province. We found that emissions were significantly redistributed among provinces owing to interprovincial trade. Large amounts of emissions were embodied in the imports of eastern regions from northern and central regions, and these were determined by differences in regional economic status and environmental policy. It is suggested that measures should be introduced to reduce air pollution by integrating cross-regional consumers and producers within national agreements to encourage efficiency improvement in the supply chain and optimize consumption structure internationally. The consumption-based air pollutant emission inventory developed in this work can be further used to attribute pollution to various economic activities and final demand types with the aid of air quality models

Corrigendum to "Assessment of China's virtual air pollution transport embodied in trade by using a consumption-based emission inventory" published in Atmos. Chem. Phys., 15, 5443-5456, 2015

No abstract available

The B→Xsl+l−B\to X_sl^+l^- and B→XsγB\to X_s \gamma decays with the fourth generation

If the fourth generation fermions exist, the new quarks could influence the branching ratios of the decays of $B\to X_s \gamma$ and $B\to X_sl^+l^-$. We obtain two solutions of the fourth generation CKM factor $V^{*}_{t^{'}s}V_{t^{'}b}$ from the decay of $B\to X_s \gamma$. We use these two solutions to calculate the new contributions of the fourth generation quark to Wilson coefficients of the decay of $B\to X_sl^+l^-$. The branching ratio and the forward-backward asymmetry of the decay of $B\to X_sl^+l^-$ in the two cases are calculated. Our results are quite different from that of SM in one case, almost same in another case. If Nature chooses the formmer, the $B$ meson decays could provide a possible test of the forth generation existence.Comment: 10 pages, 5 figure

Theory of the thermoelectricity of intermetallic compounds with Ce or Yb ions

The thermoelectric properties of intermetallic compounds with Ce or Yb ions are explained by the single-impurity Anderson model which takes into account the crystal-field splitting of the 4{\it f} ground-state multiplet, and assumes a strong Coulomb repulsion which restricts the number of {\it f} electrons or {\it f} holes to $n_f\leq 1$ for Ce and $n_f^{hole}\leq 1$ for Yb ions. Using the non-crossing approximation and imposing the charge neutrality constraint on the local scattering problem at each temperature and pressure, the excitation spectrum and the transport coefficients of the model are obtained. The thermopower calculated in such a way exhibits all the characteristic features observed in Ce and Yb intermetallics. Calculating the effect of pressure on various characteristic energy scales of the model, we obtain the $(T,p)$ phase diagram which agrees with the experimental data on CeRu$_{2}$Si$_2$, CeCu$_{2}$Si$_2$, CePd$_{2}$Si$_2$, and similar compounds. The evolution of the thermopower and the electrical resistance as a function of temperature, pressure or doping is explained in terms of the crossovers between various fixed points of the model and the redistribution of the single-particle spectral weight within the Fermi window.Comment: 13 pages, 11 figure

Floating of Extended States and Localization Transition in a Weak Magnetic Field

We report results of a numerical study of non-interacting electrons moving in a random potential in two dimensions in the presence of a weak perpendicular magnetic field. We study the topological properties of the electronic eigenstates within a tight binding model. We find that in the weak magnetic field or strong randomness limit, extended states float up in energy. Further, the localization length is found to diverge at the insulator phase boundary with the same exponent $\nu$ as that of the isolated lowest Landau band (high magnetic field limit).Comment: RevTex, 4 pages, 3 figures available upon reques

Conductance fluctuations at the integer quantum Hall plateau transition

We study numerically conductance fluctuations near the integer quantum Hall effect plateau transition. The system is presumed to be in a mesoscopic regime, with phase coherence length comparable to the system size. We focus on a two-terminal conductance G for square samples, considering both periodic and open boundary conditions transverse to the current. At the plateau transition, G is broadly distributed, with a distribution function close to uniform on the interval between zero and one in units of e^2/h. Our results are consistent with a recent experiment by Cobden and Kogan on a mesoscopic quantum Hall effect sample.Comment: minor changes, 5 pages LaTex, 7 postscript figures included using epsf; to be published Phys. Rev. B 55 (1997

Magnetization and Level Statistics at Quantum Hall Liquid-Insulator Transition in the Lattice Model

Statistics of level spacing and magnetization are studied for the phase diagram of the integer quantum Hall effect in a 2D finite lattice model with Anderson disorder.Comment: 4 pages, 6 figure

Localization and conductance fluctuations in the integer quantum Hall effect: Real--space renormalization group approach

We consider the network model of the integer quantum Hall effect transition. By generalizing the real--space renormalization group procedure for the classical percolation to the case of quantum percolation, we derive a closed renormalization group (RG) equation for the universal distribution of conductance of the quantum Hall sample at the transition. We find an approximate solution of the RG equation and use it to calculate the critical exponent of the localization length and the central moments of the conductance distribution. The results obtained are compared with the results of recent numerical simulations.Comment: 17 pages, RevTex, 7 figure