Why not "do simple things in a simple way": Use of the Pap test as the first step in screening genetic stability for human cultured stem cell therapy?

The aim of this study was to analyze adipose tissue-derived mesenchymal stem cells (AT-MSCs) using the Pap test as a first screening step to evaluate genetic stability. Human adipose tissue from six healthy female donors was obtained from elective liposuction procedures. The cells were isolated, cultivated at P2/P3, characterized by flow cytometric analysis, and differentiation induced. The AT-MSCs were stained by Papanicolaou staining and analyzed according to the Bethesda classification, and viability-apoptosis relationships were evaluated. The results of the Pap test for Sample I indicated high-grade alterations consistent with genetic instability; for Samples II-V, atypical cells of undetermined significance; and for Sample VI, normal cells. These results demonstrate the potential of using the Pap test as an initial screening step to evaluate the genetic stability of cultured AT-MSCs and also suggest its use for other adherent cells such as embryonic stem cells or induced pluripotent stem cells

QUALIDADE MICROBIOLÓGICA E FÍSICO-QUÍMICA DO LEITE PASTEURIZADO PRODUZIDO NA REGIÃO OESTE DO PARANÁ

O presente trabalho teve como objetivo avaliar a qualidade do leite pasteurizado produzido em Palotina - PR, comparando-se os resultados com os padrões da legislação nacional vigente, através de análises microbiológicas e físico-químicas. Foram coletadas aleatoriamente, 40 amostras de leites pasteurizados de diversos estabelecimentos comerciais de Palotina(PR). Deste total 16 amostras foram da marca X (08 tipo B e 08 desnatados, com inspeção estadual - SIP), 16 amostras da marca Y (08 tipo C e 08 desnatados com inspeção federal - SIF) e 08 amostras da marca Z (tipo C com inspeção federal - SIF). Após a coleta, as amostras foram enviadas imediatamente ao Laboratório de Controle Microbiológico de Água e Alimentos LACOMA, da UFPR Campus Palotina e estocadas em geladeira por um período máximo de 1 hora até o início das análises. Os resultados demonstraram que o produto estava dentro dos padrões aceitáveis para a contagem de mesófilos, psicrotróficos e coliformes fecais. Foi encontrado elevado percentual de amostras de leite peroxidase negativa (12 amostras 30%). Foi possível identificar 07 leites aguados, 05 suspeitos de aguagem, 01 com desnate parcial e 01 suspeito de desnate. Com os resultados obtidos pode-se concluir que as três marcas de leite comercializadas em Palotina apresentaram-se dentro dos padrões microbiológicos o que pode estar relacionado ao alto percentual de superaquecimento e que os resultados físico-químicos foram, de forma geral, insatisfatórios. Microbiological and physicalchemistry quality of pasteurized milks produced in the west region, Parana Abstract A survey has been carried out by means of microbiological and physical chemistry methods on the quality of pasteurized samples of milk from Palotina, Parana State, Brazil. Forty samples of pasteurized milks were gathered at several retail stores from Palotina. Sixteen samples labeled as X - 8 B and 8 skimmed milk, 16 samples labeled as Y - 8 C and 8 skimmed milk, and 8 samples labeled as Z - C milk, were analyzed at the Federal University of Paraná Laboratory for Water and Food Microbiological Control at Palotina Campus. After being stored at the refrigerator for one hour before the beginning of the analysis. According to the results found, all the milk samples analyzed were within the acceptable limits for mesophilic, psychrotroph and fecal coliform countings. An important data was the high percentage, as much as 30%, of negative peroxidase samples. In regard to the physical chemistry analysis carried out - fat, density at 15o C, total dry extract, unfat dry extract, cryoscopic index, phosphatase and peroxidase, the results were in general not satisfactories

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics

Xenarthrans – anteaters, sloths, and armadillos – have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with 24 domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, ten anteaters, and six sloths. Our dataset includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data-paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the south of the USA, Mexico, and Caribbean countries at the northern portion of the Neotropics, to its austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n=5,941), and Cyclopes sp. has the fewest (n=240). The armadillo species with the most data is Dasypus novemcinctus (n=11,588), and the least recorded for Calyptophractus retusus (n=33). With regards to sloth species, Bradypus variegatus has the most records (n=962), and Bradypus pygmaeus has the fewest (n=12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other datasets of Neotropical Series which will become available very soon (i.e. Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans dataset

Uso e cobertura da terra no entorno de corpos d'água no litoral sul catarinense e os conflitos com as Áreas de Preservação Permanente.

Uso e cobertura da terra no entorno de corpos d'água no litoral sul catarinense e os conflitos com as Áreas de Preservação Permanent

Educação patrimonial em espaços formais e informais no município de Santa Vitória do Palmar, RS, Brasil

This article aims to present the experiences developed in formal and informal educationalspaces of the Patrimonial Education Program, related to the project for the implementation of wind energy complex in the municipality of Santa Vitória do Palmar, Rio Grande do Sul state, Brazil. The theoretical basis on Patrimonial Education and Regional Cultural Heritage was discussedwith teachers and students of elementary school. The program included activities in the classroom and in informal spaces, involvingteachers andstudents and the community, enabling interaction and the exchange of knowledge between students, teachers, community and researchers involved in the process. The results showedthat the initiative is valid, fruitful and has the support of official institutions that regulate the licensing process for engineering projects, with regard to the preservation, conservation and democratization of the use, enjoyment and knowledge of what belongs to the whole society, its cultural heritageO artigo se propõe a apresentar as experiências educativas relacionadas às atividades do Programa de Educação Patrimonial e Ambiental em espaços formais e informais de educação, vinculado ao projeto de implantação de um complexo eólico de energia, no município de Santa Vitória do Palmar, RS, Brasil. O projeto contemplou o desenvolvimento de atividades no espaço da sala de aula propiciando a interação entre educandos, educadores, comunidade e pesquisadores. Neste ambiente foi trabalhado um embasamento teórico sobre o conceito de Educação Patrimonial, assim como Patrimônio Cultural e Ambiental regional. Os debates acadêmicos sobre os temas demonstraram que a iniciativa é válida, profícua e tem o respaldo e o respeito das instituições oficiais que se dedicam a preservar, conservar e democratizar o uso, desfrute e conhecimento daquilo que é de toda a sociedade, sua identidade patrimonial.El objectivo de este trabajo es presentar las experiencias desarrolladas en espacios formales e informales de educación del Programa de Educación del Patrimonio, vinculado al proyecto para la implementación del complejo de energía eólica en el municipio de Santa Vitória do Palmar, RS, Brasil. La base teórica sobre la educación del patrimonio y el patrimonio cultural regional se trabajó con maestros y estudiantes de la escuela primaria. El programa incluyó actividades en las clases y en espacios informales con maestros y estudiantes y con la comunidad, permitiendo la interacción y el intercambio de conocimientos entre estudiantes, maestros, comunidad e investigadores involucrados en el proceso. Los resultados obtenidos demostraron que la iniciativa es válida, fructífera y cuenta con el apoyo de instituciones oficiales que regulan el proceso de concesión de licencias para obras de ingeniería, en lo que respecta a la preservación, conservación y democratización del uso, disfrute y conocimiento de lo que pertenece al conjunto. sociedad, su patrimonio cultural