The effect of ball carrying on the sprinting speed of international rugby union players

Speed is considered to be a highly valuable ability in rugby union. One unique aspect of rugby is that players need to be effective at sprinting while carrying a rugby ball. Previous research reported that amateur club players were slower while sprinting with the ball, than without. The purpose of the current research was to examine how sprinting while carrying a ball affected the sprinting speed of international rugby players. Twenty-six international players performed 6 x 40 m sprints under three conditions: Ball One Hand (B1H), Ball Two Hands (B2H) and No Ball (NB). Timing gates were placed at the 0 m, 10 m, 30 m and the 40m mark of the sprint. The 0-10m was used to examine initial acceleration; 30-40 m was used to examine maximal velocity and the 10-30 m section to analyze the acceleration up to maximal velocity. Comparisons were also made between backs and forwards. Backs were found to be faster than forwards at each of the splits for the NB, B1H and B2H conditions (0.04 - 0.08 s,

A genome-wide association study demonstrates significant genetic variation for fracture risk in Thoroughbred racehorses

Background: Thoroughbred racehorses are subject to non-traumatic distal limb bone fractures that occur during racing and exercise. Susceptibility to fracture may be due to underlying disturbances in bone metabolism which have a genetic cause. Fracture risk has been shown to be heritable in several species but this study is the first genetic analysis of fracture risk in the horse. Results: Fracture cases (n = 269) were horses that sustained catastrophic distal limb fractures while racing on UK racecourses, necessitating euthanasia. Control horses (n = 253) were over 4 years of age, were racing during the same time period as the cases, and had no history of fracture at the time the study was carried out. The horses sampled were bred for both flat and National Hunt (NH) jump racing. 43,417 SNPs were employed to perform a genome-wide association analysis and to estimate the proportion of genetic variance attributable to the SNPs on each chromosome using restricted maximum likelihood (REML). Significant genetic variation associated with fracture risk was found on chromosomes 9, 18, 22 and 31. Three SNPs on chromosome 18 (62.05 Mb – 62.15 Mb) and one SNP on chromosome 1 (14.17 Mb) reached genome-wide significance (p <0.05) in a genome-wide association study (GWAS). Two of the SNPs on ECA 18 were located in a haplotype block containing the gene zinc finger protein 804A (ZNF804A). One haplotype within this block has a protective effect (controls at 1.95 times less risk of fracture than cases, p = 1 × 10-4), while a second haplotype increases fracture risk (cases at 3.39 times higher risk of fracture than controls, p = 0.042). Conclusions: Fracture risk in the Thoroughbred horse is a complex condition with an underlying genetic basis. Multiple genomic regions contribute to susceptibility to fracture risk. This suggests there is the potential to develop SNP-based estimators for genetic risk of fracture in the Thoroughbred racehorse, using methods pioneered in livestock genetics such as genomic selection. This information would be useful to racehorse breeders and owners, enabling them to reduce the risk of injury in their horses

Reconstructing sea-level change in the Falkland Islands (Islas Malvinas) using salt-marsh foraminifera, diatoms and testate amoebae

Proxy records of past sea-level change provide a means of extending sea-level histories from tide gauges into the pre-industrial period. This is especially valuable in the South Atlantic region where sea-level data are limited to only a few tide-gauge records. Multi-proxy approaches to sea-level reconstruction are relatively rare but have distinct benefits when groups of micro-organisms are sparse or under-represented in modern or fossil sediments. Here, we address this challenge by utilising surface foraminifera, testate amoebae and diatoms from a salt marsh at Swan Inlet, East Falkland. All three micro-organism groups occupied distinct vertical niches in the contemporary salt-marsh. We investigated the relative performance of each group of micro-organisms in providing a sea-level reconstruction using individual (group-specific) regression models and with a multi-proxy regression model that combined all three groups. Foraminifera alone were not a suitable proxy. Surveyed sample elevations were closely matched by estimated elevations using Weighted-Average (WA) and Weighted-Average Partial-Least-Squares (WA- PLS) regressions. Relative sea-level reconstructions were derived by applying each model to microfossil assemblages recovered from a core (SI-2) from the same site. The combined transfer function yielded reconstructive precision (± 0.08 m) comparable to our best single-proxy transfer function (± 0.06 m) but only 18% of palaeo-samples were identified as having “close” or “good” analogues in the combined training data set. We highlight the benefit of a pragmatic approach to sea-level reconstructions whereby additional proxies should be employed if the use of only one proxy performs poorly across the width of the elevation gradient

Hazy with a chance of star spots: constraining the atmosphere of the young planet, K2-33b

Although all-sky surveys have led to the discovery of dozens of young planets, little is known about their atmospheres. Here, we present multi-wavelength transit data for the super Neptune-sized exoplanet, K2-33b -- the youngest (~10 Myr) transiting exoplanet to-date. We combined photometric observations of K2-33 covering a total of 33 transits spanning >2 years, taken from K2, MEarth, Hubble, and Spitzer. The transit photometry spanned from the optical to the near-infrared (0.6-4.5$\mu$m), enabling us to construct a transmission spectrum of the planet. We find that the optical transit depths are nearly a factor of two deeper than those from the near-infrared. This difference holds across multiple datasets taken over years, ruling out issues of data analysis and unconstrained systematics. Surface inhomogeneities on the young star can reproduce some of the difference, but required spot coverage fractions (>60%) are ruled out by the observed stellar spectrum(<20%). We find a better fit to the transmission spectrum using photochemical hazes, which were predicted to be strong in young, moderate-temperature, and large-radius planets like K2-33b. A tholin haze with CO as the dominant gaseous carbon carrier in the atmosphere can reasonably reproduce the data with small or no stellar surface inhomogeneities, consistent with the stellar spectrum. The HST data quality is insufficient for the detection of any molecular features. More observations would be required to fully characterize the hazes and spot properties and confirm the presence of CO suggested by current data.Comment: Accepted to AJ. 26 pages, 14 figures, 6 table

A comparison of career satisfaction amongst dental healthcare professionals across three health care systems: Comparison of data from the United Kingdom, New Zealand and Trinidad & Tobago

BACKGROUND: The aim of this study was to compare the expressed levels of career satisfaction of three groups of comparable dental healthcare professionals, working in Trinidad, the United Kingdom and New Zealand. METHODS: Three questionnaire surveys were carried out of comparable dental healthcare professionals. Dental nurses in Trinidad and dental therapists in the UK and New Zealand. Questionnaires were sent to all registered dental nurses or dental therapists. RESULTS: Career satisfaction was lowest amongst Dental Therapists working in Trinidad and Tobago. Approximately 59% of the Therapists working in New Zealand reported stated that they felt they were not a valued member of the dental team, the corresponding proportion in the United Kingdom was 32%, and for Trinidad 39%. CONCLUSION: Dental therapists working in different healthcare systems report different levels of satisfaction with their career

Population Structure Shapes Copy Number Variation in Malaria Parasites.

If copy number variants (CNVs) are predominantly deleterious, we would expect them to be more efficiently purged from populations with a large effective population size (Ne) than from populations with a small Ne. Malaria parasites (Plasmodium falciparum) provide an excellent organism to examine this prediction, because this protozoan shows a broad spectrum of population structures within a single species, with large, stable, outbred populations in Africa, small unstable inbred populations in South America and with intermediate population characteristics in South East Asia. We characterized 122 single-clone parasites, without prior laboratory culture, from malaria-infected patients in seven countries in Africa, South East Asia and South America using a high-density single-nucleotide polymorphism/CNV microarray. We scored 134 high-confidence CNVs across the parasite exome, including 33 deletions and 102 amplifications, which ranged in size from <500 bp to 59 kb, as well as 10,107 flanking, biallelic single-nucleotide polymorphisms. Overall, CNVs were rare, small, and skewed toward low frequency variants, consistent with the deleterious model. Relative to African and South East Asian populations, CNVs were significantly more common in South America, showed significantly less skew in allele frequencies, and were significantly larger. On this background of low frequency CNV, we also identified several high-frequency CNVs under putative positive selection using an FST outlier analysis. These included known adaptive CNVs containing rh2b and pfmdr1, and several other CNVs (e.g., DNA helicase and three conserved proteins) that require further investigation. Our data are consistent with a significant impact of genetic structure on CNV burden in an important human pathogen

The WID-EC test for the detection and risk prediction of endometrial cancer

The incidence of endometrial cancer is rising. Measures to identify women at risk and to detect endometrial cancer earlier are required to reduce the morbidity triggered by the aggressive treatment required for advanced endometrial cancer. We developed the WID-EC (Women's cancer risk IDentification-Endometrial Cancer) test, which is based on DNA methylation at 500 CpG sites, in a discovery set of cervical liquid-based cytology samples from 1,086 women with and without an endometrial cancer (217 cancer cases and 869 healthy controls) with a worse prognosis (grade 3 or ≥stage IB). We validated the WID-EC test in an independent external validation set of 64 endometrial cancer cases and 225 controls. We further validated the test in 150 healthy women (prospective set) who provided a cervical sample as part of the routine Swedish cervical screening programme, 54 of whom developed endometrial cancer within three years of sample collection. The WID-EC test identified women with endometrial cancer with a receiver operator characteristic area under the curve (AUC) of 0.92 (95% CI: 0.88-0.97) in the external set and of 0.82 (95% CI: 0.74-0.89) in the prospective validation set. Using an optimal cutoff, cancer cases were detected with a sensitivity of 86% and a specificity of 90% in the external validation set, and a sensitivity and specificity of 52% and 98% respectively in the prospective validation set. The WID-EC test can identify women with or at risk of endometrial cancer

Experiences of parenting and clinical intervention for mothers affected by personality disorder: a pilot qualitative study combining parent and clinician perspectives

Background: Evidence-based parenting programmes are recommended for the treatment of child mental health difficulties. Families with complex psychosocial needs show poorer retention and outcomes when participating in standard parenting programmes. The Helping Families Programme (HFP) is a 16-week community-based parenting intervention designed to meet the needs of these families, including families with parental personality disorder. This study aimed to explore the help seeking and participatory experiences of parents with a diagnosis of personality disorder. It further aimed to examine the acceptability of referral and intervention processes for the HFP from the perspectives of (i) clinicians referring into the programme; and (ii) referred parents. Method: Semi-structured interviews were conducted with parents recruited to receive HFP (n = 5) as part of a research case series and the referring NHS child and adolescent mental health service (CAMHS) clinicians (n = 5). Transcripts were analysed using Interpretive Phenomenological Analysis. Results: Four themes were identified for parents: (i) the experience of parenthood, (ii) being a parent affected by personality disorder, (iii) experience of the intervention, and (iv) qualities of helping. Three themes emerged for clinicians: (i) challenges of addressing parental need, (ii) experience of engaging parents with personality disorders and (iii) limited involvement during HFP. Comparison of parent and clinician themes led to the identification of two key interlinked themes: (i) concerns prior to receiving the intervention, and (ii) the challenges of working together without a mutual understanding. Conclusions: This pilot study identifies potentially significant challenges of working with parents affected by personality disorder and engaging them in HFP and other similar interventions. Results have important wider clinical implications by highlighting potential barriers to engagement and participation and providing insights on how these barriers might be overcome. Findings have been used to inform the referral and intervention processes of a pilot RCT and further intervention development