Infection risk of Monilinia fructicola on stone fruit during cold storage and immersion in the dump tank

Monilinia spp. is the main pathogen responsible for postharvest losses of stone fruit. Several studies have examined the conditions for Monilinia spp. infection in the field, but very limited information is available about postharvest. Storing fruit for 1 day in the cold room or dumping fruit in a water tank are the most common handling operations during the postharvest of fruit. Then, the aim of this study was to investigate the risk of Monilinia fructicola infection for two peach and one nectarine cultivars during cold storage and water dump operations. A new methodology was performed using a dry inoculum of M. fructicola. A set of fruit was used as control to demonstrated that at 20 °C 60% relative humidity (RH) was not able to infect fruit, however, the disease was developed when was already infected. In addition, M. fructicola was able to infect and develop disease in fruit at 20 °C 100 RH. The storage of fruit with the presence of M. fructicola conidia on their surface for up to 30 days at 0 °C 100% RH or 4 °C 100% RH did not suppose an important risk of infection since only 3.3 and 3.8%, respectively of fruit were already infected. Overall, all treatments tested with the water dump operation gave optimal conditions to M. fructicola to infect fruit when it was superficially dry inoculated or it was immersed with water contaminated with conidia, increasing the need for water disinfection. In addition, when fruit was immersed in water free from M. fructicola conidia, the postharvest operation gave optimal conditions to develop infections already produced.info:eu-repo/semantics/acceptedVersio

Enhanced shelf-life of the formulated biocontrol agent Bacillus amyloliquefaciens CPA-8 combining diverse packaging strategies and storage conditions

Two effective biocontrol products (named as BA3 and BA4) based on Bacillus amyloliquefaciens CPA-8 have been reported as a potential alternative to chemical applications against brown rot caused by Monilinia spp. on stone fruit. To have practical use, this study aimed to describe the best packaging strategies (bags or flasks, atmosphere, and temperature of storage) to not only guarantee efficacy but also stability and ease of application of the products to be handled through the normal channels of distribution and storage. In terms of the viability neither the BA3 nor the BA4 product has been compromised after twelve months of storage. However, storage at 4 °C affected the stability and visual aspect of both CPA-8 formulations, mainly associated not only to the increase of RH but also aw. Moreover, it should be pointed out that flasks did not conserve refrigerated BA3 samples in a suitable way, since RH and aw increased noticeably making their visual properties unsightly after 10 months of cold storage. At that time, the BA4 products were better preserved at 4 °C when packaged in flasks. Finally, this study also demonstrated that the most suitable packaging conditions for long-term storability (stored at 22 °C) did not show any negative effect in the biocontrol efficacy of CPA-8 in nectarines artificially infected with M. fructicola and provide suitable product delivery and field application. In conclusion, these results contribute to the final stage of development of these two CPA-8 products, practically ready for registration, thus contributing to the environmental-friendly management of postharvest diseases in stone fruit.info:eu-repo/semantics/acceptedVersio

Impact of climate change environmental conditions on the resilience of different formulations of the biocontrol agent Candida sake CPA‐1 on grapes

Biocontrol agents have become componentsof integrated crop protection systemsfor controlling economically important fungal pathogens.Candida sakeCPA-1 is abiocontrol agent of fungal pathogens of fruits, both pre- and post-harvest. Whilethe efficacy of different formulations have been examined previously, few studieshave considered the resilience of different formulations under changing climaticconditions of elevated temperature, drought stress and increased atmospheric CO2.This study examined the effect of (a) temperature9RH9elevated CO2(400vs1000 ppm) on the temporal establishment and viability of two dry and one liquidC. sakeCPA-1 formulations on grape berry surfaces; (b) temperature stress (25vs35°C); and (c) elevated CO2levels. Results indicated that temperature, RH and CO2concentration influenced the establishment and viability of the formulations butthere was no significant difference between formulations. For the combined three-component factors, increased temperature (35°C) and lower RH (40%) reduced theviable populations on grapes. The interaction with elevated CO2improved theestablishment of viable populations of the formulations tested. Viable populationsgreater than Log 4 CFUs per g were recovered from the grape surfaces suggestingthat these had conserved resilience for control ofBotrytisrot in grapes.info:eu-repo/semantics/acceptedVersio

Caracterización de la Falla de Alhama de Murcia mediante MASW

The deep structure of the Alhama de Murcia Fault (AMF) is characterized by means of the analysis of surface waves identified in shallow high resolution seismic reflection data. Multichannel analysis of surface waves (MASW) is used to unravel the 2-D S-wave velocity model and image the depth geometry of the fault system. The study area includes segments of the fault located in La Salud area. Two approaches have been used to achieve a well constrained velocity model in the AMF fault zone. Conventional seismic reflection processing work flow has been used to clean the seismic data and increase its S/N ratio. Occam’s approach has been used to invert the digitized surface wave dispersion curves. 1D shear wave velocity-depth profiles were estimated in shot and CDP domains. Relatively well resolved 2D velocity-depth models were obtained by com- posite of the 1D Velocity-depth functions. These composite 2D-velocity models are able to constrain the depth geometry of the fault zone up to 100 m depth. The fault zone is indicated by a relatively broad low velocity anomaly that correlates with the fault’s surface expression.La estructura profunda de la falla de Alhama de Murcia (AMF) ha sido caracterizada mediante el análisis de ondas superficiales identificados en datos de reflexión sísmica superficial de alta resolución. El análisis multicanal de ondas de superficie (MASW) es usado para desentrañar un modelo 2D de Vs y para obtener imágenes de la geometría del sistema de fallas en profundidad. El área de estudio incluye segmentos de la AMF ubicados en el área de La Salud. Se han utilizado dos enfoques para lograr modelos de velocidad bien restringidos de la zona de falla. Por un lado, se han limpiado los datos utilizado un flujo de procesado convencional diseñado para aumentar su ratio de señal/ruido. Por el otro, se ha usado el enfoque de Occam para invertir las curvas de dispersión de ondas de superficie digitalizadas. Los perfiles 1D de profundidad de las Vs fueron estimados en los dominios de disparo y de CDP. Se obtuvieron modelos de velocidad-profundidad 2D relativamente bien resueltos mediante la interpolación de las funciones de velocidad-profundidad 1D. Estos modelos de velocidad 2D ayu- dan a restringir en profundidad la geometría de la zona de falla hasta los 100 m que se caracteriza por una anomalía de baja velocidad relativamente amplia que se correlaciona en la superficie con la expresión de la falla.Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEMinisterio de Ciencia e Innovación (MICINN)ICTJA-CSICMinistry of Education and Culture of the Republic of Indonesiapu

Aplicação de métodos geofísicos em Arqueologia: desenvolvimento de metodologias eficazes de prospecção geofísica aplicadas a diferentes ambientes arqueológicos

The use of geophysical methods applied to Archaeology, in the exploration of buried structures in the sub-surface, is an imperative need in the previous steps to excavations or archaeological surveys, playing an important role in the delimitation of archaeological sites, making it more effective protective actions archaeological heritage. With regard to planning an archaeological exploration, geophysical methods significantly reduce the runtime and operating costs, guiding with acceptable accuracy for local archaeologist digging and what to look for. The development of efficient methods in geophysical exploration, applied to different archaeological environments usually requires the crossing of different geophysical techniques. The methodologies for the application of geophysical surveys shall concern either in the steps of the data acquisition either in the steps of the obtained data processing and in the result representation. This work presents the results for two techniques, georadar and magnetometry, in different archaeological locations, showing the differences between the different archaeological sites, whose features are different

Caracterización sísmica de la falla de Alhama de Murcia

La falla de Alhama de Murcia (AMF) es una de las fallas más activas de la Península Ibérica y fue la causante del terremoto de Lorca de 2011 que significó numerosos daños materiales e incluso víctimas mortales. Esta falla oblicua en dirección NE-SO, que se extiende por más de 100 km, ha sido estudiada en superficie pero poco se sabe de su estructura en profundidad. Esto es especialmente importante para poder interpretar de forma correcta los numerosos datos paleosísmicos que se están obteniendo, ya que conocer la relación estructural en profundidad de las distintas ramas que forman la AMF es esencial. Por este motivo, y dentro del marco del proyecto InterGEO, se ha realizado una campaña de adquisición de datos sísmicos 2D a lo largo de la AMF, centrándose en los segmentos más interesantes en la zona de Lorca-Totana. Aquí se presentan los los resultados preliminares del procesado de algunos de los perfiles adquiridos en esta campaña que muestran la primera imagen estructural de la falla en profundidad y que permitirá entender el comportamiento de dicha falla, así como determinar su relevancia desde el punto de vista del riesgo sísmico.Este trabajo ha podido ser realizado gracias a la financiación de los proyectos CGL2014-56548-P, 2009-SGR-1595, 2009-SGR-1595.Peer Reviewe

Predicted ecological niches and environmental resilience of different formulations of the biocontrol yeast Candida sake CPA-1 using the Bioscreen C

Environmental resilience of biocontrol microorganisms has been a major bottleneck in the development of effective formulations. Candida sake is an effective biocontrol agent (BCA) against Penicillium expansum, Botrytis cinerea or Rhizopus stolonifer, and different formulations of the BCA have been optimised recently. The objective of this study was to compare the relative tolerance of different dry and liquid formulations of the biocontrol yeast C. sake CPA-1 to interacting environmental conditions using the Bioscreen C. Initially, the use of this automated turbidimetric method was optimised for use with different formulations of the biocontrol yeast. The best growth curves were obtained for the C. sake CPA-1 strain when grown in a synthetic grape juice medium under continuous shaking and with an initial concentration of 105 CFUs ml−1. All the formulations showed a direct relationship between optical density values and yeast concentrations. Temperature (15–30 °C) and water activity (aw; 0.94–0.99) influenced the yeast resilience most profoundly, whereas the effect of pH (3–7) was minimal. In general, the liquid formulation grew faster in more interacting environmental conditions but only the yeast cells in the dry potato starch formulation could grow in some stress conditions. This rapid screening method can be used for effective identification of the resilience of different biocontrol formulations under interacting ecological abiotic conditions.info:eu-repo/semantics/acceptedVersio

Predicted ecological niches and environmental resilience of different formulations of the biocontrol yeast Candida sake CPA-1 using the Bioscreen C

Environmental resilience of biocontrol microorganisms has been a major bottleneck in the development of effective formulations. Candida sake is an effective biocontrol agent (BCA) against Penicillium expansum, Botrytis cinerea or Rhizopus stolonifer, and different formulations of the BCA have been optimised recently. The objective of this study was to compare the relative tolerance of different dry and liquid formulations of the biocontrol yeast C. sake CPA-1 to interacting environmental conditions using the Bioscreen C. Initially, the use of this automated turbidimetric method was optimised for use with different formulations of the biocontrol yeast. The best growth curves were obtained for the C. sake CPA-1 strain when grown in a synthetic grape juice medium under continuous shaking and with an initial concentration of 105 CFUs ml−1. All the formulations showed a direct relationship between optical density values and yeast concentrations. Temperature (15–30 °C) and water activity (aw; 0.94–0.99) influenced the yeast resilience most profoundly, whereas the effect of pH (3–7) was minimal. In general, the liquid formulation grew faster in more interacting environmental conditions but only the yeast cells in the dry potato starch formulation could grow in some stress conditions. This rapid screening method can be used for effective identification of the resilience of different biocontrol formulations under interacting ecological abiotic conditions

Impact of climate change environmental conditions on the resilience of different formulations of the biocontrol agent Candida sake CPA‐1 on grapes

Biocontrol agents have become components of integrated crop protection systems for controlling economically important fungal pathogens. Candida sake CPA‐1 is a biocontrol agent of fungal pathogens of fruits, both pre‐ and post‐harvest. While the efficacy of different formulations have been examined previously, few studies have considered the resilience of different formulations under changing climatic conditions of elevated temperature, drought stress and increased atmospheric CO2. This study examined the effect of (a) temperature × RH × elevated CO2 (400 vs 1000 ppm) on the temporal establishment and viability of two dry and one liquid C. sake CPA‐1 formulations on grape berry surfaces; (b) temperature stress (25 vs 35°C); and (c) elevated CO2 levels. Results indicated that temperature, RH and CO2 concentration influenced the establishment and viability of the formulations but there was no significant difference between formulations. For the combined three‐component factors, increased temperature (35°C) and lower RH (40%) reduced the viable populations on grapes. The interaction with elevated CO2 improved the establishment of viable populations of the formulations tested. Viable populations greater than Log 4 CFUs per g were recovered from the grape surfaces suggesting that these had conserved resilience for control of Botrytis rot in grapes

GPR Clutter Reflection Noise-Filtering through Singular Value Decomposition in the Bidimensional Spectral Domain

Usually, in ground-penetrating radar (GPR) datasets, the user defines the limits between the useful signal and the noise through standard filtering to isolate the effective signal as much as possible. However, there are true reflections that mask the coherent reflectors that can be considered noise. In archaeological sites these clutter reflections are caused by scattering with origin in subsurface elements (e.g., isolated masonry, ceramic objects, and archaeological collapses). Its elimination is difficult because the wavelet parameters similar to coherent reflections and there is a risk of creating artefacts. In this study, a procedure to filter the clutter reflection noise (CRN) from GPR datasets is presented. The CRN filter is a singular value decomposition-based method (SVD), applied in the 2D spectral domain. This CRN filtering was tested in a dataset obtained from a controlled laboratory environment, to establish a mathematical control of this algorithm. Additionally, it has been applied in a 3D-GPR dataset acquired in the Roman villa of Horta da Torre (Fronteira, Portugal), which is an uncontrolled environment. The results show an increase in the quality of archaeological GPR planimetry that was verified via archaeological excavation