1,805 research outputs found
Influence of Pure Dephasing on Emission Spectra from Single Photon Sources
We investigate the light-matter interaction of a quantum dot with the
electromagnetic field in a lossy microcavity and calculate emission spectra for
non-zero detuning and dephasing. It is found that dephasing shifts the
intensity of the emission peaks for non-zero detuning. We investigate the
characteristics of this intensity shifting effect and offer it as an
explanation for the non-vanishing emission peaks at the cavity frequency found
in recent experimental work.Comment: Published version, minor change
Fungi in the healthy human gastrointestinal tract
Many species of fungi have been detected in the healthy human gut; however, nearly half of all taxa reported have only been found in one sample or one study. Fungi capable of growing in and colonizing the gut are limited to a small number of species, mostly Candida yeasts and yeasts in the family Dipodascaceae (Galactomyces, Geotrichum, Saprochaete). Malassezia and the filamentous fungus Cladosporium are potential colonizers; more work is needed to clarify their role. Other commonly-detected fungi come from the diet or environment but either cannot or do not colonize (Penicillium and Debaryomyces species, which are common on fermented foods but cannot grow at human body temperature), while still others have dietary or environmental sources (Saccharomyces cerevisiae, a fermentation agent and sometime probiotic; Aspergillus species, ubiquitous molds) yet are likely to impact gut ecology. The gut mycobiome appears less stable than the bacterial microbiome, and is likely subject to environmental factors
Fungi in the healthy human gastrointestinal tract
Many species of fungi have been detected in the healthy human gut; however, nearly half of all taxa reported have only been found in one sample or one study. Fungi capable of growing in and colonizing the gut are limited to a small number of species, mostly Candida yeasts and yeasts in the family Dipodascaceae (Galactomyces, Geotrichum, Saprochaete). Malassezia and the filamentous fungus Cladosporium are potential colonizers; more work is needed to clarify their role. Other commonly-detected fungi come from the diet or environment but either cannot or do not colonize (Penicillium and Debaryomyces species, which are common on fermented foods but cannot grow at human body temperature), while still others have dietary or environmental sources (Saccharomyces cerevisiae, a fermentation agent and sometime probiotic; Aspergillus species, ubiquitous molds) yet are likely to impact gut ecology. The gut mycobiome appears less stable than the bacterial microbiome, and is likely subject to environmental factors
The human gut mycobiome: pitfalls and potentials — a mycologist\u27s perspective
We have entered the Age of the Microbiome, with new studies appearing constantly and whole journals devoted to the human microbiome. While bacteria outnumber other gut microbes by orders of magnitude, eukaryotes are consistently found in the human gut, and are represented primarily by the fungi. Compiling 36 studies spanning from 1917 to 2015, we found at least 267 distinct fungal taxa have been reported from the human gut, and seemingly every new study includes one or more fungi not previously described from this niche. This diversity, while impressive, is illusory. If we examine gut fungi, we will quickly observe a division between a small number of commonly detected species (Candida yeasts, Saccharomyces and yeasts in the Dipodascaceae, and Malassezia species), and a long tail of taxa which have only been reported once. Furthermore, an investigation into the ecology of these rare species reveals that many of them are incapable of colonization or long-term persistence in the gut. This paper examines what we know and have yet to learn about the fungal component of the gut microbiome, or “mycobiome”, and an overview of methods. We address the potential of the field while introducing some caveats, and argue for the necessity of including mycologists in mycobiome studies
Sequence-based Methods for Detecting and Evaluating the Human Gut Mycobiome
We surveyed the fungal microbiota in 16 fecal samples from healthy humans with a vegetarian diet. Fungi were identified using molecular cloning, 454 pyrosequencing and a Luminex analyte specific reagent (ASR) assay, all targeting the ITS region of the rRNA genes. Fungi were detected in each fecal sample and at least 46 distinct fungal operational taxonomic units (OTUs) were detected, from two phyla — Ascomycota and Basidiomycota. Fusarium was the most abundant genus, followed by Malassezia, Penicillium, Aspergillus, and Candida. Commonly detected fungi such as Aspergillus and Penicillium, as well as known dietary fungi Agaricus bisporus and Ophiocordyceps sinensis, are presumed to be transient, allochthonous members due to their abundance in the environment or dietary associations. No single method identified the full diversity of fungi in all samples; pyrosequencing detected more distinct OTUs than the other methods, but failed to detect OTUs in some samples that were detected by cloning and/or ASR assays. ASRs were limited by the commercially available assays, but the potential to design new, optimized assays, coupled with speed and cost, makes the ASR method worthy of further study
Pseudo-Riemannian geodesic foliations by circles
We investigate under which assumptions an orientable pseudo-Riemannian
geodesic foliations by circles is generated by an -action. We construct
examples showing that, contrary to the Riemannian case, it is not always true.
However, we prove that such an action always exists when the foliation does not
contain lightlike leaves, i.e. a pseudo-Riemannian Wadsley's Theorem. As an
application, we show that every Lorentzian surface all of whose
spacelike/timelike geodesics are closed, is finitely covered by .
It follows that every Lorentzian surface contains a non-closed geodesic.Comment: 14 page
Near-infrared photoabsorption by C(60) dianions in a storage ring
We present a detailed study of the electronic structure and the stability of C(60) dianions in the gas phase. Monoanions were extracted from a plasma source and converted to dianions by electron transfer in a Na vapor cell. The dianions were then stored in an electrostatic ring, and their near-infrared absorption spectrum was measured by observation of laser induced electron detachment. From the time dependence of the detachment after photon absorption, we conclude that the reaction has contributions from both direct electron tunneling to the continuum and vibrationally assisted tunneling after internal conversion. This implies that the height of the Coulomb barrier confining the attached electrons is at least similar to 1.5 eV. For C(60)(2-) ions in solution electron spin resonance measurements have indicated a singlet ground state, and from the similarity of the absorption spectra we conclude that also the ground state of isolated C(60)(2-) ions is singlet. The observed spectrum corresponds to an electronic transition from a t(1u) lowest unoccupied molecular orbital (LUMO) of C(60) to the t(1g) LUMO+1 level. The electronic levels of the dianion are split due to Jahn-Teller coupling to quadrupole deformations of the molecule, and a main absorption band at 10723 cm(-1) corresponds to a transition between the Jahn-Teller ground states. Also transitions from pseudorotational states with 200 cm(-1) and (probably) 420 cm(-1) excitation are observed. We argue that a very broad absorption band from about 11 500 cm(-1) to 13 500 cm(-1) consists of transitions to so-called cone states, which are Jahn-Teller states on a higher potential-energy surface, stabilized by a pseudorotational angular momentum barrier. A previously observed, high-lying absorption band for C(60)(-) may also be a transition to a cone state
Setting up and modelling of overflowing fed-batch cultures of Bacillus subtilis for the production and continuous removal of lipopeptides
This work is related to the setup of overflowing exponential fed-batch
cultures (O-EFBC) derived from carbon limited EFBC dedicated to the production
of mycosubtilin, an antifungal lipopeptide belonging to the iturin family.
O-EFBC permits the continuous removal of the product from the bioreactor
achieving a complete extraction of mycosubtilin. This paper also provides a
dynamical Monod-based growth model of this process that is accurate enough to
simulate the evolution of the specific growth rate and to correlate it to the
mycosubtilin specific productivity. Two particular and dependant phenomena
related to the foam overflow are taken into account by the model: the outgoing
flow rate of a broth volume and the loss of biomass. Interestingly, the biomass
concentration in the foam was found to be lower than the biomass concentration
in the bioreactor relating this process to a recycling one. Parameters of this
model are the growth yield on substrate and the maximal specific growth rate
estimated from experiments led at feed rates of 0.062, 0.071 and 0.086 h --1.
The model was extrapolated to five additional experiments carried out at feed
rates of 0.008, 0.022, 0.040, 0.042 and 0.062 h --1 enabling the correlation of
the mean specific growth rates with productivity results. Finally, a feed rate
of 0.086 h --1 corresponding to a mean specific growth rate of 0.070 h --1
allowed a specific productivity of 1.27 mg of mycosubtilin g --1 of dried
biomass h --1
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