Viscoelastic repetitive creep and recovery in bituminous materials

Abstract Repetitive creep and recovery tests in bituminous materials- asphalt and asphalt paving mix are studied at high service temperatures. The non-exponential- stretched exponential type of continuous retardation spectrum is defined and used for the calculation of creep compliance function in repetitive shear and tensile creep and recovery experiments and also in the wheel tracking test on asphalt paving mix. It is shown that the used model, with only five adjustable parameters, can describe the test quite well. The model is also adequate for the description of the composite compliance function constructed from the linear viscoelastic data and the apparently nonlinear compliance function obtained in the wheel tracking test, for asphalt paving mix

Analysis of protein composition of rabbit aqueous humor following two different cataract surgery incision procedures using 2-DE and LC-MS/MS

<p>Abstract</p> <p>Background</p> <p>The aqueous humor (AH), a liquid of the anterior and posterior chamber of the eye, comprises many proteins with various roles and important biological functions. Many of these proteins have not been identified yet and their functions in AH are still unknown. Recently, our laboratory published the protein database of AH obtained from healthy rabbits which expanded known protein identifications by 65%. Our present study extends our previous work and analyses AH following two types of cataract surgery incision procedures (clear corneal and limbal incisions) by using two dimensional gel electrophoresis (2-DE) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Although both incision protocols are commonly used during cataract surgeries, the difference in protein composition and their release into AH following each surgery has never been systematically compared and remains unclear. The first step, which is the focus of this work, is to assess the scale of the protein change, at which time does maximum release occurs and when possible, to identify protein changes.</p> <p>Results</p> <p>Samples of AH obtained prior to surgery and at different time points (0.5, 2, 12, 24 and 48 hours) following surgery (n = 3/protocol) underwent protein concentration determination, 2-DE and LC-MS/MS. There was a large (9.7 to 31.2 mg/mL) and rapid (~0.5 hour) influx of proteins into AH following either incision with a return to baseline quantities after 12 hours and 24 hours for clear corneal and limbal incision, respectively. We identified 80 non-redundant proteins, and compared to our previous study on healthy AH, 67.5% of proteins were found to be surgery-specific. In addition, 51% of those proteins have been found either in clear corneal (20%) or limbal incision (31%) samples.</p> <p>Conclusions</p> <p>Our results imply that a mechanism of protein release into AH after surgery is a global response to the surgery rather than increase in amount of protective proteins found in healthy AH and a mechanism of protein release for each type of incision procedure could be different. Although the total protein concentration was increased (at 0.5 and 2 hour time points and between types of surgery) many of 2-DE protein spots were similar based on 2-DE and MS analyses, and only a small number of protein spots changed with either the time points or surgical conditions (0.4 -1.9%). This suggests that the high protein content is due to an increase in the concentration of the same proteins with only a few unique proteins being altered per time point and with the different surgery type. This is the first report on the comparison of AH protein composition following two different cataract surgery procedures and it establishes the basis for better understanding of protein release into AH during events such as cataract surgery or other possible intervention to the eyes.</p

The effect of stochastic perturbations on plankton transport by internal solitary waves

Internal solitary and solitary-like waves are a commonly observed feature of density stratified natural waters, including lakes and the coastal ocean. Since such waves induce significant currents throughout the water column they can be responsible for significant transport of both passive and swimming biota. We consider simple models of moving zooplankton based on the Langevin equation. The small amplitude randomness significantly alters the nature of particle motion. In particular, passage through the wave leads to strongly non Gaussian particle distributions. When the plankton swims to return to its equilibrium photic level, a steady state that balances randomness, swimming and wave-induced motions is possible. We discuss possible implications of this steady state for organisms that feed on plankton

A multi-parent recombinant inbred line population of C. elegans allows identification of novel QTLs for complex life history traits

Background The nematode Caenorhabditis elegans has been extensively used to explore the relationships between complex traits, genotypes, and environments. Complex traits can vary across different genotypes of a species, and the genetic regulators of trait variation can be mapped on the genome using quantitative trait locus (QTL) analysis of recombinant inbred lines (RILs) derived from genetically and phenotypically divergent parents. Most RILs have been derived from crossing two parents from globally distant locations. However, the genetic diversity between local C. elegans populations can be as diverse as between global populations and could thus provide means of identifying genetic variation associated with complex traits relevant on a broader scale. Results To investigate the effect of local genetic variation on heritable traits, we developed a new RIL population derived from 4 parental wild isolates collected from 2 closely located sites in France: Orsay and Santeuil. We crossed these 4 genetically diverse parental isolates to generate a population of 200 multi-parental RILs and used RNA-seq to obtain sequence polymorphisms identifying almost 9000 SNPs variable between the 4 genotypes with an average spacing of 11 kb, doubling the mapping resolution relative to currently available RIL panels for many loci. The SNPs were used to construct a genetic map to facilitate QTL analysis. We measured life history traits such as lifespan, stress resistance, developmental speed, and population growth in different environments, and found substantial variation for most traits. We detected multiple QTLs for most traits, including novel QTLs not found in previous QTL analysis, including those for lifespan and pathogen responses. This shows that recombining genetic variation across C. elegans populations that are in geographical close proximity provides ample variation for QTL mapping. Conclusion Taken together, we show that using more parents than the classical two parental genotypes to construct a RIL population facilitates the detection of QTLs and that the use of wild isolates facilitates the detection of QTLs. The use of multi-parent RIL populations can further enhance our understanding of local adaptation and life history trade-offs

Nonlinear interfacial waves in a constant-vorticity planar flow over variable depth

Exact Lagrangian in compact form is derived for planar internal waves in a two-fluid system with a relatively small density jump (the Boussinesq limit taking place in real oceanic conditions), in the presence of a background shear current of constant vorticity, and over arbitrary bottom profile. Long-wave asymptotic approximations of higher orders are derived from the exact Hamiltonian functional in a remarkably simple way, for two different parametrizations of the interface shape.Comment: revtex, 4.5 pages, minor corrections, summary added, accepted to JETP Letter

Mode 2 waves on the continental shelf : ephemeral components of the nonlinear internal wavefield

Author Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 115 (2010): C07001, doi:10.1029/2009JC005605.Shoreward propagating, mode 2 nonlinear waves appear sporadically in mooring records obtained off the coast of New Jersey in the summer of 2006. Individual mode 2 packets were tracked between two moorings separated by 1 km; however, packets could not be tracked between moorings separated by greater distances from one another (∼10 km). The inability to track individual packets large distances through the mooring array combined with detailed observations from a ship suggest that these waves are short lived. The evolution of the ship-tracked wave group was recorded using acoustic backscatter, acoustic Doppler current profilers, and turbulence profiling. The leading mode 2 wave quickly changed form and developed a tail of short, small-amplitude mode 1 waves. The wavelength of the mode 1 oscillations agreed with that expected for a copropagating tail on the basis of linear theory. Turbulent dissipation in the mixed layer and radiation of the short mode 1 waves contributed to rapid energy loss in the leading mode 2 wave, consistent with the observed decay rate and short life span of only a few hours. The energy in the leading mode 2 wave was 10–100 times smaller than the energy of mode 1 nonlinear internal waves observed during the experiment; however, the magnitudes of wave-localized turbulent dissipation were similar.This work was funded by the Office of Naval Research