817 research outputs found
Nonlinear self-interaction of plane gravitational waves
Recently Mendonca and Cardoso [Phys. Rev. D, vol. 66, 104009 (2002)]
considered nonlinear gravitational wave packets propagating in flat space-time.
They concluded that the evolution equation - to third order in amplitude -
takes a similar form to what arises in nonlinear optics. Based on this
equation, the authors found that nonlinear gravitational waves exhibit
self-phase modulation and high harmonic generation leading to frequency
up-shifting and spectral energy dilution of the gravitational wave energy. In
this Brief Report we point out the fact - a possibility that seems to have been
overlooked by Mendonca and Cardoso - that the nonlinear terms in the evolution
equation cancels and, hence, that there is no amplitude evolution of the pulse.
Finally we discuss scenarios where these nonlinearities may play a role.Comment: 2 pages, to appear in Phys. Rev.
The bldC developmental locus of Streptomyces coelicolor encodes a member of a family of small DNA-binding proteins related to the DNA-binding domains of the MerR family.
The bldC locus, required for formation of aerial hyphae in Streptomyces coelicolor, was localized by map-based cloning to the overlap between cosmids D17 and D25 of a minimal ordered library. Subcloning and sequencing showed that bldC encodes a member of a previously unrecognized family of small (58- to 78-residue) DNA-binding proteins, related to the DNA-binding domains of the MerR family of transcriptional activators. BldC family members are found in a wide range of gram-positive and gram-negative bacteria. Constructed {Delta}bldC mutants were defective in differentiation and antibiotic production. They failed to form an aerial mycelium on minimal medium and showed severe delays in aerial mycelium formation on rich medium. In addition, they failed to produce the polyketide antibiotic actinorhodin, and bldC was shown to be required for normal and sustained transcription of the pathway-specific activator gene actII-orf4. Although {Delta}bldC mutants produced the tripyrrole antibiotic undecylprodigiosin, transcripts of the pathway-specific activator gene (redD) were reduced to almost undetectable levels after 48 h in the bldC mutant, in contrast to the bldC+ parent strain in which redD transcription continued during aerial mycelium formation and sporulation. This suggests that bldC may be required for maintenance of redD transcription during differentiation. bldC is expressed from a single promoter. S1 nuclease protection assays and immunoblotting showed that bldC is constitutively expressed and that transcription of bldC does not depend on any of the other known bld genes. The bldC18 mutation that originally defined the locus causes a Y49C substitution that results in instability of the protein
The guanine nucleotide exchange factor RIC8 regulates conidial germination through Gα proteins in Neurospora crassa.
Heterotrimeric G protein signaling is essential for normal hyphal growth in the filamentous fungus Neurospora crassa. We have previously demonstrated that the non-receptor guanine nucleotide exchange factor RIC8 acts upstream of the Gα proteins GNA-1 and GNA-3 to regulate hyphal extension. Here we demonstrate that regulation of hyphal extension results at least in part, from an important role in control of asexual spore (conidia) germination. Loss of GNA-3 leads to a drastic reduction in conidial germination, which is exacerbated in the absence of GNA-1. Mutation of RIC8 leads to a reduction in germination similar to that in the Δgna-1, Δgna-3 double mutant, suggesting that RIC8 regulates conidial germination through both GNA-1 and GNA-3. Support for a more significant role for GNA-3 is indicated by the observation that expression of a GTPase-deficient, constitutively active gna-3 allele in the Δric8 mutant leads to a significant increase in conidial germination. Localization of the three Gα proteins during conidial germination was probed through analysis of cells expressing fluorescently tagged proteins. Functional TagRFP fusions of each of the three Gα subunits were constructed through insertion of TagRFP in a conserved loop region of the Gα subunits. The results demonstrated that GNA-1 localizes to the plasma membrane and vacuoles, and also to septa throughout conidial germination. GNA-2 and GNA-3 localize to both the plasma membrane and vacuoles during early germination, but are then found in intracellular vacuoles later during hyphal outgrowth
Nonlinear coupled Alfv\'{e}n and gravitational waves
In this paper we consider nonlinear interaction between gravitational and
electromagnetic waves in a strongly magnetized plasma. More specifically, we
investigate the propagation of gravitational waves with the direction of
propagation perpendicular to a background magnetic field, and the coupling to
compressional Alfv\'{e}n waves. The gravitational waves are considered in the
high frequency limit and the plasma is modelled by a multifluid description. We
make a self-consistent, weakly nonlinear analysis of the Einstein-Maxwell
system and derive a wave equation for the coupled gravitational and
electromagnetic wave modes. A WKB-approximation is then applied and as a result
we obtain the nonlinear Schr\"{o}dinger equation for the slowly varying wave
amplitudes. The analysis is extended to 3D wave pulses, and we discuss the
applications to radiation generated from pulsar binary mergers. It turns out
that the electromagnetic radiation from a binary merger should experience a
focusing effect, that in principle could be detected.Comment: 20 pages, revtex4, accepted in PR
Fast Magnetosonic Waves Driven by Gravitational Waves
The propagation of a gravitational wave (GW) through a magnetized plasma is
considered. In particular, we study the excitation of fast magnetosonic waves
(MSW) by a gravitational wave, using the linearized general-relativistic
hydromagnetic equations. We derive the dispersion relation for the plasma,
treating the gravitational wave as a perturbation in a Minkowski background
space-time. We show that the presence of gravitational waves will drive
magnetosonic waves in the plasma and discuss the potential astrophysical
implications.Comment: 12 pages, 2 figures, Astronomy and Astrophysics in pres
Charged multifluids in general relativity
The exact 1+3 covariant dynamical fluid equations for a multi-component
plasma, together with Maxwell's equations are presented in such a way as to
make them suitable for a gauge-invariant analysis of linear density and
velocity perturbations of the Friedmann-Robertson-Walker model. In the case
where the matter is described by a two component plasma where thermal effects
are neglected, a mode representing high-frequency plasma oscillations is found
in addition to the standard growing and decaying gravitational instability
picture. Further applications of these equations are also discussed.Comment: 14 pages (example added), to appear in Class. Quantum Gra
Transverse Wave Propagation in Relativistic Two-fluid Plasmas in de Sitter Space
We investigate transverse electromagnetic waves propagating in a plasma in
the de Sitter space. Using the 3+1 formalism we derive the relativistic
two-fluid equations to take account of the effects due to the horizon and
describe the set of simultaneous linear equations for the perturbations. We use
a local approximation to investigate the one-dimensional radial propagation of
Alfv\'en and high frequency electromagnetic waves and solve the dispersion
relation for these waves numerically.Comment: 19 pages, 12 figure
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Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.
G protein-coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization
Very high frequency gravitational wave background in the universe
Astrophysical sources of high frequency gravitational radiation are
considered in association with a new interest to very sensitive HFGW receivers
required for the laboratory GW Hertz experiment. A special attention is paid to
the phenomenon of primordial black holes evaporation. They act like black body
to all kinds of radiation, including gravitons, and, therefore, emit an
equilibrium spectrum of gravitons during its evaporation. Limit on the density
of high frequency gravitons in the Universe is obtained, and possibilities of
their detection are briefly discussed.Comment: 14 page
Reassessment of the Lineage Fusion Hypothesis for the Origin of Double Membrane Bacteria
In 2009, James Lake introduced a new hypothesis in which reticulate phylogeny reconstruction is used to elucidate the origin of Gram-negative bacteria (Nature 460: 967–971). The presented data supported the Gram-negative bacteria originating from an ancient endosymbiosis between the Actinobacteria and Clostridia. His conclusion was based on a presence-absence analysis of protein families that divided all prokaryotes into five groups: Actinobacteria, Double Membrane bacteria (DM), Clostridia, Archaea and Bacilli. Of these five groups, the DM are by far the largest and most diverse group compared to the other groupings. While the fusion hypothesis for the origin of double membrane bacteria is enticing, we show that the signal supporting an ancient symbiosis is lost when the DM group is broken down into smaller subgroups. We conclude that the signal detected in James Lake's analysis in part results from a systematic artifact due to group size and diversity combined with low levels of horizontal gene transfer.Exobiology Program (U.S.) (Grant NNX08AQ10G)Assembling the Tree of Life (Program) (Grant DEB 0830024
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