Implementation and Validation of the Roche Light Cycler 480 96-Well Plate Platform as a Real-Time PCR Assay for the Quantitative Detection of Cytomegalovirus (CMV) in Clinical Specimens Using the Luminex MultiCode ASRs System.

Allogenic stem-cell therapies benefit patients in the treatment of multiple diseases; however, the side effects of stem-cell therapies (SCT) derived from the concomitant use of immune suppression agents often include triggering infection diseases. Thus, analysis is required to improve the detection of pathogen infections in SCT. We develop a polymerase chain reaction (PCR)-based methodology for the qualitative real-time DNA detection of cytomegalovirus (CMV), with reference to herpes simplex virus types 1 (HSVI), Epstein-Barr virus (EBV), and varicella-zoster virus (VZV) in blood, urine, solid tissues, and cerebrospinal fluid. This real-time PCR of 96-well plate format provides a rapid framework as required by the Food and Drug Administration (FDA) for clinical settings, including the processing of specimens, reagent handling, special safety precautions, quality control criteria and analytical accuracy, precisely reportable range (analyst measurement range), reference range, limit of detection (LOD), analytical specificity established by interference study, and analyte stability. Specifically, we determined the reportable range (analyst measurement range) with the following criteria: CMV copies ≥200 copies/mL; report copy/mL value; CMV copies ≤199 copies/mL; report detected but below quantitative range; CMV copies = 0 with report <200 copies/mL. That is, with reference range, copy numbers (CN) per milliliter (mL) of the LOD were determined by standard curves that correlated Ct value and calibrated standard DNA panels. The three repeats determined that the measuring range was 1E2~1E6 copies/mL. The standard curves show the slopes were within the range -2.99 to -3.65 with R2 ≥ 0.98. High copy (HC) controls were within 0.17-0.18 log differences of DNA copy numbers; (2) low copy (LC) controls were within 0.17-0.18 log differences; (3) LOD was within 0.14-0.15 log differences. As such, we set up a fast, simple, inexpensive, sensitive, and reliable molecular approach for the qualitative detection of CMV pathogens. Conclusion: This real-time PCR of the 96-well plate format provides a rapid framework as required by the FDA for clinical settings

Velocities of Reacting Boron Particles within a Solid Fuel Ramjet Combustion Chamber

A 2D-laser doppler velocimeter was used to measure velocities of reacting boron (B) particles during the combustion of a metallised solid fuel slab inside a 20-combustion chamber. The solid fuel hydroxyl-terminated polybutadiene (HTPB) was enriched with B particles to increase its specific heat. To obtain information on the combustion process and on the movement of B particles, their velocities were measured. The experiments were performed at ambient pressure. The behaviour of the B particles concerning the exit velocities from the fuel slab has been discussed on the basis of the experimental results

New data about Early Cretaceous Ginkgoales from southwestern Europe (middle-late Albian of Spain)

We present in this work a leaf-type corresponding to the Ginkgoales genus Sphenobaiera recorded in deposits from the middle-upper Albian (Lower Cretaceous) of northeastern Spain. The fossil leaf shows a widely dissected lamina that forks regularly six times ending at the apex in sixty-four tiny wedge-shaped segments, which are characters similar to those in Sphenobaiera pecten from the Middle Jurassic of England. The exceptional preservation of this fossil leaf, which preserves all segments in connection with its base, in addition to taphonomical and sedimentological data, indicates a nearby location for the parent plant, which grew near the shores of a tidally-influenced fluvial environment. A palaeoenvironmental reconstruction of the sub-environments where the different types of Ginkgoales known from the Albian of northeastern Spain developed is also presented. The presence of a variety of different types of Ginkgoales in middle-upper Albian deposits from Spain represents a new contribution to the knowledge of the palaeoenvironmental conditions for this region of southwestern Eurasia during the late Early Cretaceous

Catabolite repression of the citST two-component system in Bacillus subtilis

In Bacillus subtilis, expression of the citrate transporter CitM is under strict control. Transcription of the citM gene is induced by citrate in the medium mediated by the CitS-CitT two-component system and repressed by rapidly degraded carbon sources mediated by carbon catabolite repression (CCR). In this study, we demonstrate that citST genes are part of a bicistronic operon. The promoter region was localized in a stretch of 58 base pairs upstream of the citS gene by deletion experiments. Transcription of the operon was repressed in the presence of glucose by the general transcription factor CcpA. A distal consensus cre site in the citS-coding sequence was implicated in the mechanism of repression. Furthermore, this repression was relieved in Bacillus subtilis mutants deficient in CcpA or Hpr/Crh, components essential to CCR. Thus, we demonstrate that CCR represses the expression of the citST operon, which is responsible for the induction of citM, through the cre site located 1326 bp from transcriptional start site of citST

La figura de la Malinche en la literatura hispana del siglo XX: La aprendiz de bruja de Alejo Carpentier, Todos los gatos son pardos de Carlos Fuentes y Jubileo en el Zócalo de Ramón Sender

The article analyses the figure of La Malinche in three least-known theatrical texts of the 20th century Hispanic Literature: La aprendiz de bruja of the cuban writer, Alejo Carpentier, Todos los gatos son pardos of the mexican Carlos Fuentes and the spanish Ramón Sender’s Jubileo en el Zócalo. Starting from the historical events and the reality will be examined her enigmatic rol, her tragic and contradictory destination in these fictions. The different views of the three works make it possible to present the indian woman’s and her nation’s identity