Diversity of leaf katydids (Orthoptera: Tettigoniidae: Phaneropterinae) of Dzanga-Ndoki National Park, Central African Republic, with selected records from other African countries.

Forty-four species of Phaneropterinae are recorded from Dzanga-Ndoki National Park in the Central African Republic. Eight species collected within the park are described as new to science: Phlaurocentrum morettoi n. sp., P. paratuberosum n. sp., P. elegans n. sp., Myllocentrum raggei n. sp., Poreuomena sanghensis n. sp., Cestromoecha longicerca n. sp., C. magnicerca n. sp., and Goetia purpurea n. sp. An additional new species from Guinea is described from specimens preserved in the Museo Nacional de Ciencias Naturales of Madrid, Poreuomena huxleyi n. sp. Eurycorypha stylata St\ue5l, 1873 is recorded for the first time from Burkina Faso and Brycoptera lobata Ragge, 1981 from Ivory Coast. The following synonyms are established: Enochletica affinis Bol\uedvar, 1906 is synonymized with Enochletica ostentatrix Karsch, 1896, Poreuomena gladiator Bol\uedvar, 1906 is synonymized with Poreuomena forcipata Sj\uf6stedt, 1902, and Azamia doriae (Griffini, 1906) is synonymized with Azamia biplagiata Bol\uedvar, 1906. In addition, morphological characters of previously unknown females of Phaneroptera maculosa Ragge, 1956 and Eurycorypha canaliculata Karsch, 1890, and males of Myllocentrum stigmosum (Karsch, 1896) and Cestromoecha crassipes (Karsch, 1890) are described. The presence of titillators in four African genera (Gelotopoia, Brycoptera, Phlaurocentrum and Azamia) is recorded for the first time. In the genus Zeuneria, a sub-equally bilobed, dorsally curved, dorso-lateral abdominal appendage is described for the first time. This new structure apparently delimits an abdominal gland of unknown function on the second tergite

Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 2 ligand binding assays - impact of 25-hydroxyvitamin D

From PubMed via Jisc Publications RouterHistory: received 2021-05-29, revised 2021-07-15, accepted 2021-07-23Publication status: aheadofprintFunder: NIH Office of the Director; Grant(s): not applicableAn interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D [25(OH)D ], 25-hydroxyvitamin D [25(OH)D ], 3-epi-25-hydroxyvitamin D [3-epi-25(OH)D ], and 24R,25-dihydroxyvitamin D [24R,25(OH) D ] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias compared to the assigned target values and using linear regression analysis of the test assay mean results and the target values. Only 50% of the ligand binding assays achieved the VDSP criterion of mean % bias ≤ |± 5%|. For the 13 unique ligand binding assays evaluated in this study, only 4 assays were consistently within ± 5% mean bias and 4 assays were consistently outside ± 5% mean bias regardless of the laboratory performing the assay. Based on multivariable regression analysis using the concentrations of individual vitamin D metabolites in the 50 single-donor samples, most assays underestimate 25(OH)D and several assays (Abbott, bioMérieux, DiaSorin, IDS-EIA, and IDS-iSYS) may have cross-reactivity from 24R,25(OH) D . The results of this interlaboratory study represent the most comprehensive comparison of 25(OH)D ligand binding assays published to date and is the only study to assess the impact of 24R,25(OH) D content using results from a reference measurement procedure. [Abstract copyright: © 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.