The effect of amino acid deprivation on the transfer of iron through Caco-2 cell monolayers

Funding Source Rural and Environmental Scientific and Analytical Services, the Scottish Government Acknowledgments We thank Dr Helen Hayes for her technical support during this project. We also thank Dr Christine Kennedy for her involvement at the beginning of this project. This study was funded by Rural and Environmental Scientific and Advisory Service of Scottish Government.Peer reviewedPostprin

SPIRE imaging of M 82: Cool dust in the wind and tidal streams

M 82 is a unique representative of a whole class of galaxies, starbursts with superwinds, in the Very Nearby Galaxy Survey with Herschel. In addition, its interaction with the M 81 group has stripped a significant portion of its interstellar medium from its disk. SPIRE maps now afford better characterization of the far-infrared emission from cool dust outside the disk, and sketch a far more complete picture of its mass distribution and energetics than previously possible. They show emission coincident in projection with the starburst wind and in a large halo, much more extended than the PAH band emission seen with Spitzer. Some complex substructures coincide with the brightest PAH filaments, and others with tidal streams seen in atomic hydrogen. We subtract the far-infrared emission of the starburst and underlying disk from the maps, and derive spatially-resolved far-infrared colors for the wind and halo. We interpret the results in terms of dust mass, dust temperature, and global physical conditions. In particular, we examine variations in the dust physical properties as a function of distance from the center and the wind polar axis, and conclude that more than two thirds of the extraplanar dust has been removed by tidal interaction, and not entrained by the starburst wind

Thulium and ytterbium-doped titanium oxide thin films deposited by ultrasonic spray pyrolysis

Thin films of thulium and ytterbium-doped titanium oxide were grown by metal-organic spray pyrolysis deposition from titanium(IV)oxide bis(acetylacetonate), thulium(III) tris(2,2,6,6-tetramethyl-3,5-heptanedionate) and ytterbium(III) tris(acetylacetonate). Deposition temperatures have been investigated from 300{\deg}C to 600{\deg}C. Films have been studied regarding their crystallity and doping quality. Structural and composition characterisations of TiO2:Tm,Yb were performed by electron microprobe, X-ray diffraction and Fourier transform infrared spectroscopy. The deposition rate can reach 0.8 \mum/h. The anatase phase of TiO2 was obtained after synthesis at 400{\deg}C or higher. Organic contamination at low deposition temperature is eliminated by annealing treatments.Comment: 6 pages, 6 figure

The effect of maternal iron deficiency on zinc and copper levels and on genes of zinc and copper metabolism during pregnancy in the rat

Fe deficiency is relatively common in pregnancy and has both short- and long-term consequences. However, little is known about the effect on the metabolism of other micronutrients. A total of fifty-four female rats were fed control (50 mg Fe/kg) or Fe-deficient diets (7·5 mg/kg) before and during pregnancy. Maternal liver, placenta and fetal liver were collected at day 21 of pregnancy for Cu and Zn analysis and to measure expression of the major genes of Cu and Zn metabolism. Cu levels increased in the maternal liver (P=0·002) and placenta (P=0·018) of Fe-deficient rats. Zn increased (P&lt;0·0001) and Cu decreased (P=0·006) in the fetal liver. Hepatic expression of the Cu chaperones antioxidant 1 Cu chaperone (P=0·042) and cytochrome c oxidase Cu chaperone (COX17, P=0·020) decreased in the Fe-deficient dams, while the expression of the genes of Zn metabolism was unaltered. In the placenta, Fe deficiency reduced the expression of the chaperone for superoxide dismutase 1, Cu chaperone for superoxide dismutase (P=0·030), ceruloplasmin (P=0·042) and Zn transport genes, ZRT/IRT-like protein 4 (ZIP4, P=0·047) and Zn transporter 1 (ZnT1, P=0·012). In fetal liver, Fe deficiency increased COX17 (P=0·020), ZRT/IRT-like protein 14 (P=0·036) and ZnT1 (P=0·0003) and decreased ZIP4 (P=0·004). The results demonstrate that Fe deficiency during pregnancy has opposite effects on Cu and Zn levels in the fetal liver. This may, in turn, alter metabolism of these nutrients, with consequences for development in the fetus and the neonate.</p

Fresh concrete pumping arrest investigation for thixotropy by a CFD modelling apporach

Concrete pumping operations determine construction speed, finishing quality, durability and even structural integrity. When pumping operations cannot be continued, most problems occur due to complex time-dependent transformations. This causes significant industrial costs (e.g. material and delay). Since time-dependent aspects are currently not fully understood and cannot be predicted, a way to quantify time-dependent aspects is needed. Therefore, we make an attempt by numerical simulation by comparing thixotropic cases with different pumping arresting times. After an introduction to fresh concrete rheology and numerical modelling, ten representative thixotropy cases are analysed. Despite some unresolved numerical instabilities, the numerical framework allows to estimate pumping pressure peaks after resting time. The results evaluate a thixotropy model, which is generally applicable for less thixotropic SCC’s. It is clear that flow re-initiation after rest in concrete pumping is poorly understood. Numerical simulation could be one approach for further analysis and is potentially important for practice. Future work such as simulation of concrete mixers, pressure increase after pumping arrest, formwork pressure decay and leakage are therefore recommended