Cloning of the copper-inducible metallothionein (cmt) promoter from Neurospora crassa.

There are only a limited number of vectors with inducible promoters that are convenient for in vivo expression in Neurospora crassa. Promoters have been identified and cloned that are induced with blue-light (bli-4; Pietschmann et al 1991 Fungal Genetics Newsl. 38:85-6) or by quinic acid (qa-2; Campbell et al 1994 Fungal Genetics Newsl. 41:20-1). Constitutive promoters have also been used, derived from the beta-tubulin gene bml (Nakano et al 1993 Fungal Genetics Newsl. 40:54-6). The glucose-repressible promoter of grg-1 has also been used (Nakano ibid; Pall and Brunelli 1994 Fungal Genetics Newsl. 41:63-4). The promoter of the N. crassa copper metallothionein gene (cmt) is capable of induction levels of at least 100-fold (Munger et al. 1987 J. Biol. Chem. 262:7363-7) and has been used to express tyrosinase and laccase (Kupper et al. 1990 Curr. Genet. 18:331-5; Schilling et al. 1992 Curr. Genet. 22:197-203)

The Wilson-Garnjobst heterokaryon incompatibility tester strains of Neurospora crassa contain modifiers which influence growth rate of heterokaryons and distort segregation ratios.

Recent interest and accelerated research into the genetics of heterokaryon incompatibility (HI) in Neurospora crassa has led to increased use of the original Wilson-Garnjobst HI tester strains available from FGSC (1994 Catalog of Strains, Part VII.D.1.). We have found inconsistencies and abnormalities in both growth of heterokaryons and segregation of markers in crosses using these strains. First noticed was a lack of vigor and incomplete complementation of markers in forced heterokaryons when compared to compatible heterokaryons with known Oak Ridge (OR) background. Secondly, skewed allele ratios were recorded in crosses between the Wilson-Garnjobst strains and strains with OR background. Perkins and Bjorkman raised a cautionary note about these strains (1978 Neurospora Newsl. 25:24-25), however, they concentrated primarily on the scot mutant present in these and other strains originating from the Rockefeller-Lindegren (RL) background. We have attempted to further characterize the erratic behavior of Wilson-Garnjobst strains and determine if the scot mutant or other modifiers of HI are responsible

Post-discharge care following acute kidney injury: quality improvement in primary care

BACKGROUND: Over the past decade, targeting acute kidney injury (AKI) has become a priority to improve patient safety and health outcomes. Illness complicated by AKI is common and is associated with adverse outcomes including high rates of unplanned hospital readmission. Through national patient safety directives, NHS England has mandated the implementation of an AKI clinical decision support system in hospitals. In order to improve care following AKI, hospitals have also been incentivised to improve discharge summaries and general practices are recommended to establish registers of people who have had an episode of illness complicated by AKI. However, to date, there is limited evidence surrounding the development and impact of interventions following AKI. DESIGN: We conducted a quality improvement project in primary care aiming to improve the management of patients following an episode of hospital care complicated by AKI. All 31 general practices within a single NHS Clinical Commissioning Group were incentivised by a locally commissioned service to engage in audit and feedback, education training and to develop an action plan at each practice to improve management of AKI. RESULTS: AKI coding in general practice increased from 28% of cases in 2015/2016 to 50% in 2017/2018. Coding of AKI was associated with significant improvements in downstream patient management in terms of conducting a medication review within 1 month of hospital discharge, monitoring kidney function within 3 months and providing written information about AKI to patients. However, there was no effect on unplanned hospitalisation and mortality. CONCLUSION: The findings suggest that the quality improvement intervention successfully engaged a primary care workforce in AKI-related care, but that a higher intensity intervention is likely to be required to improve health outcomes. Development of a real-time audit tool is necessary to better understand and minimise the impact of the high mortality rate following AKI

High-Throughput Sequencing of Six Bamboo Chloroplast Genomes: Phylogenetic Implications for Temperate Woody Bamboos (Poaceae: Bambusoideae)

BACKGROUND: Bambusoideae is the only subfamily that contains woody members in the grass family, Poaceae. In phylogenetic analyses, Bambusoideae, Pooideae and Ehrhartoideae formed the BEP clade, yet the internal relationships of this clade are controversial. The distinctive life history (infrequent flowering and predominance of asexual reproduction) of woody bamboos makes them an interesting but taxonomically difficult group. Phylogenetic analyses based on large DNA fragments could only provide a moderate resolution of woody bamboo relationships, although a robust phylogenetic tree is needed to elucidate their evolutionary history. Phylogenomics is an alternative choice for resolving difficult phylogenies. METHODOLOGY/PRINCIPAL FINDINGS: Here we present the complete nucleotide sequences of six woody bamboo chloroplast (cp) genomes using Illumina sequencing. These genomes are similar to those of other grasses and rather conservative in evolution. We constructed a phylogeny of Poaceae from 24 complete cp genomes including 21 grass species. Within the BEP clade, we found strong support for a sister relationship between Bambusoideae and Pooideae. In a substantial improvement over prior studies, all six nodes within Bambusoideae were supported with ≥0.95 posterior probability from Bayesian inference and 5/6 nodes resolved with 100% bootstrap support in maximum parsimony and maximum likelihood analyses. We found that repeats in the cp genome could provide phylogenetic information, while caution is needed when using indels in phylogenetic analyses based on few selected genes. We also identified relatively rapidly evolving cp genome regions that have the potential to be used for further phylogenetic study in Bambusoideae. CONCLUSIONS/SIGNIFICANCE: The cp genome of Bambusoideae evolved slowly, and phylogenomics based on whole cp genome could be used to resolve major relationships within the subfamily. The difficulty in resolving the diversification among three clades of temperate woody bamboos, even with complete cp genome sequences, suggests that these lineages may have diverged very rapidly

The effect of cash transfers on mental health: Opening the black box – a study from South Africa

There is a gap in the literature in understanding how cash transfer programmes affect mental health. We aim to fill this gap by conceptualising and estimating the mediation effects of an unconditional cash transfer programme on mental health. We use a sample of 4,535 adults living below the South African poverty line in four waves (2008–2014) of the South African National Income Dynamics Study. We use information on individual exposure to South Africa's largest unconditional cash transfer programme, the Child Support Grant. Mental health is measured by the 10-item version of the Centre for Epidemiological Depression Scale. We use the product of the coefficient method for the mediation analysis in combination with instrumental variable estimation. We find that physical health and lifestyle factors mediate the relationship of the unconditional cash transfer programme, each explaining about eight percent and 16% of the total positive effect. Our findings show that individuals living in poverty make investment decisions that are positive for their mental health, which has strong implications for policy makers

Validation of the SF12 mental and physical health measure for the population from a low-income country in sub-Saharan Africa

Introduction The Short Form Survey 12-item (SF12) mental and physical health version has been applied in several studies on populations from Sub-Saharan Africa. However, the SF12 has not been computed and validated for these populations. We address in this paper these gaps in the literature and use a health intervention example in Malawi to show the importance of our analysis for health policy. Methods We firstly compute the weights of the SF12 physical and mental health measure for the Malawian population using principal component analysis on a sample of 2838 adults from wave four (2006) of Malawian Longitudinal Study of Aging (MLSFH). We secondly test the construct validity of our computed and the US-population weighted SF12 measures using regression analysis and Fixed Effect estimation on waves four, seven (2012) and eight (2013) of the MLSFH. Finally, we use a Malawian cash transfer programme to exemplify the implications of using US- and Malawi-weighted SF12 mental health measures in policy evaluation. Results We find that the Malawian SF12 health measure weighted by our computed Malawian population weights is strongly associated with other mental health measures (Depression:-0.501, p =  0.1). The weightings affect the size of the impacts across mental health quantiles suggesting that the weighting scheme matters for empirical health policy analysis. Conclusion Mental health shows more pronounced associations with the physical health dimension in a Low-Income Country like Malawi compared to the US. This is important for the construct validity of the SF12 health measures and has strong implications in health policy analysis. Further analysis is required for the physical health dimension of the SF12