The Effect of Diffuse Light on Crops

Light is not evenly distributed in Dutch glass greenhouses, but this can be improved with diffuse light. Modern greenhouse coverings are able to transform most of the light entering the greenhouse into diffuse light. Wageningen UR Greenhouse Horticulture has studied the effect of diffuse light on crops for several years. Modelling and experimental studies showed that crops such as fruit vegetables with a high plant canopy as well as ornamentals with a small plant canopy can utilize diffuse light better than direct light. Diffuse light penetrates the middle layers of a high-grown crop and results in a better horizontal light distribution in the greenhouse. Diffuse light is absorbed to a better degree by the middle leaf layers of cucumber, resulting in a higher photosynthesis. The actual photosynthesis of four pot plant species was found to be increased and crop temperatures were lower during high irradiation. The yield of cucumbers was increased, and the growth rate of several potted plants was increased. These investigations have resulted in a quantitative foundation for the potentials of diffuse light in Dutch horticultural greenhouses and the selection and verification of technological methods to convert direct sunlight into diffuse light

Codivergence and multiple host species use by fig wasp populations of the Ficus pollination mutualism

<p>Abstract</p> <p>Background</p> <p>The interaction between insects and plants takes myriad forms in the generation of spectacular diversity. In this association a species host range is fundamental and often measured using an estimate of phylogenetic concordance between species. Pollinating fig wasps display extreme host species specificity, but the intraspecific variation in empirical accounts of host affiliation has previously been underestimated. In this investigation, lineage delimitation and codiversification tests are used to generate and discuss hypotheses elucidating on pollinating fig wasp associations with <it>Ficus</it>.</p> <p>Results</p> <p>Statistical parsimony and AMOVA revealed deep divergences at the <it>COI </it>locus within several pollinating fig wasp species that persist on the same host <it>Ficus </it>species. Changes in branching patterns estimated using the generalized mixed Yule coalescent test indicated lineage duplication on the same <it>Ficus </it>species. Conversely, <it>Elisabethiella </it>and <it>Alfonsiella </it>fig wasp species are able to reproduce on multiple, but closely related host fig species. Tree reconciliation tests indicate significant codiversification as well as significant incongruence between fig wasp and <it>Ficus </it>phylogenies.</p> <p>Conclusions</p> <p>The findings demonstrate more relaxed pollinating fig wasp host specificity than previously appreciated. Evolutionarily conservative host associations have been tempered by horizontal transfer and lineage duplication among closely related <it>Ficus </it>species. Independent and asynchronistic diversification of pollinating fig wasps is best explained by a combination of both sympatric and allopatric models of speciation. Pollinator host preference constraints permit reproduction on closely related <it>Ficus </it>species, but uncertainty of the frequency and duration of these associations requires better resolution.</p

Chaos en orde in de wereldlandbouw

Gebrek aan elasticiteit, solidariteit en autoriteit / door P.C. van den Noort ; Internationale arbeidsverdeling en marktorde / door J. de Hoogh ; Redes 6-12-1979 Wageninge

spFRET Using Alternating Excitation and FCS Reveals Progressive DNA Unwrapping in Nucleosomes

AbstractAccessibility to DNA wrapped in nucleosomes is essential for nuclear processes such as DNA transcription. Large conformational changes in nucleosome structure are required to facilitate protein binding to target sites within nucleosomal DNA. Transient unwrapping of DNA from nucleosome ends can provide an intrinsic exposure of wrapped DNA, allowing proteins to bind DNA that would otherwise be occluded in the nucleosome. The molecular details underlying these mechanisms remain to be resolved. Here we show how DNA unwrapping occurs progressively from both nucleosome ends. We performed single-pair fluorescence resonance energy transfer (spFRET) spectroscopy with alternating laser excitation (ALEX) on nucleosomes either in free solution or confined in a gel after PAGE separation. We combined ALEX-spFRET with a correlation analysis on selected bursts of fluorescence, to resolve a variety of unwrapped nucleosome conformations. The experiments reveal that nucleosomes are unwrapped with an equilibrium constant of ∼0.2–0.6 at nucleosome ends and ∼0.1 at a location 27 basepairs inside the nucleosome, but still remain stably associated. Our findings, obtained using a powerful combination of single-molecule fluorescence techniques and gel electrophoresis, emphasize the delicate interplay between DNA accessibility and condensation in chromatin

Stokes imaging polarimetry using image restoration: A calibration strategy for Fabry-P\'{e}rot based instruments

context: The combination of image restoration and a Fabry-P\'{e}rot interferometer (FPI) based instrument in solar observations results in specific calibration issues. FPIs generally show variations over the field-of-view, while in the image restoration process, the 1-to-1 relation between pixel space and image space is lost, thus complicating any correcting for such variations. aims: We develop a data reduction method that takes these issues into account and minimizes the resulting errors. methods: By accounting for the time variations in the telescope's Mueller matrix and using separate calibration data optimized for the wavefront sensing in the MOMFBD image restoration process and for the final deconvolution of the data, we have removed most of the calibration artifacts from the resulting data. results: Using this method to reduce full Stokes data from CRISP at the SST, we find that it drastically reduces the instrumental and image restoration artifacts resulting from cavity errors, reflectivity variations, and the polarization dependence of flatfields. The results allow for useful scientific interpretation. Inversions of restored data from the $\delta$ sunspot AR11029 using the Nicole inversion code, reveal strong (~10 km/s) downflows near the disk center side of the umbra. conclusions: The use of image restoration in combination with an FPI-based instrument leads to complications in the calibrations and intrinsic limitations to the accuracy that can be achieved. We find that for CRISP, the resulting errors can be kept mostly below the polarimetric accuracy of ~10^-3. Similar instruments aiming for higher polarimetric and high spectroscopic accuracy, will, however, need to take these problems into account. keywords: Techniques: image processing, polarimetric, imaging spectroscopy, Sun: surface magnetism, sunspots, activityComment: Astronomy and Astrophysics (accepted