Solving the woolly mammoth conundrum: amino acid 15N-enrichment suggests a distinct forage or habitat

Understanding woolly mammoth ecology is key to understanding Pleistocene community dynamics and evaluating the roles of human hunting and climate change in late Quaternary megafaunal extinctions. Previous isotopic studies of mammoths’ diet and physiology have been hampered by the ‘mammoth conundrum’: woolly mammoths have anomalously high collagen δ15N values, which are more similar to coeval carnivores than herbivores and which could imply a distinct diet and (or) habitat, or a physiological adaptation. We analyzed individual amino acids from collagen of adult woolly mammoths and coeval species and discovered greater  15N enrichment in source amino acids of woolly mammoths than in most other herbivores or carnivores. Woolly mammoths consumed an isotopically distinct food source, reflective of extreme aridity, dung fertilization and (or) plant selection. This dietary signal suggests that woolly mammoths occupied a distinct habitat or forage niche relative to other Pleistocene herbivores

Isotopes, Ice Ages, and Terminal Proterozoic Earth History

Detailed correlations of ancient glacial deposits, based on temporal records of carbon acid strontium isotopes in seawater, indicate four (and perhaps five) discrete ice ages in the terminal Proterozoic Eon. The close and repeated stratigraphic relationship between C-isotopic excursions and glaciogenic rocks suggests that unusually high rates of organic carbon burial facilitated glaciation by reducing atmospheric greenhouse capacity. The emerging framework of time and environmental change contributes to the improved resolution of stratigraphic and evolutionary pattern in the early fossil record of animals.Organismic and Evolutionary Biolog

Cuando la vida anduvo errática: Un factor ambiental para la biomineralización de metazoos

The sudden appearance of biomineralized metazoans in limestone facies of the Pockenbank Member (Namibia) appears to reflect a rapid change of redox state, calcium concentration and alkalinity of the ocean. The coupling of a major biological innovation with the end of a profound carbon isotope excursion supports the view that the Shuram was a tectonic and oceanographic phenomenon, as opposed to a global diagenetic conspiracy. The temporal concordance of these profound events as preserved in strata from southern Namibia may thus be considered as exceptional geological markers for the base of the terminal Ediacaran.La repentina aparición de metazoos biomineralizados en las facies calcáreas del Miembro de Pockenbank (Namibia) parece reflejar un rápido cambio en el estado redox, la concentración de calcio y la alcalinidad del océano. La combinación de una importante innovación biológica con el final de una profunda excursión de isótopos de carbono respalda la idea de que la anomalía Shuram fue un fenómeno tectónico y oceanográfico, en contraposición a una “conspiración” diagenética mundial. La concordancia temporal de estos profundos acontecimientos, tal como se conservan en los estratos de Namibia meridional puede considerarse, por tanto, como marcadores geológicos excepcionales para la base del Ediacárico terminal

Calibration and First light of the Diabolo photometer at the Millimetre and Infrared Testa Grigia Observatory

We have designed and built a large-throughput dual channel photometer, Diabolo. This photometer is dedicated to the observation of millimetre continuum diffuse sources, and in particular, of the Sunyaev-Zel'dovich effect and of anisotropies of the 3K background. We describe the optical layout and filtering system of the instrument, which uses two bolometric detectors for simultaneous observations in two frequency channels at 1.2 and 2.1 mm. The bolometers are cooled to a working temperature of 0.1 K provided by a compact dilution cryostat. The photometric and angular responses of the instrument are measured in the laboratory. First astronomical light was detected in March 1995 at the focus of the new Millimetre and Infrared Testa Grigia Observatory (MITO) Telescope. The established sensitivity of the system is of 7 mK_RJ s^1/2$. For a typical map of at least 10 beams, with one hour of integration per beam, one can achieve the rms values of y_SZ ~ 7 10^-5 and the 3K background anisotropy Delta T/T ~ 7 10^-5, in winter conditions. We also report on a novel bolometer AC readout circuit which allows for the first time total power measurements on the sky. This technique alleviates (but does not forbid) the use of chopping with a secondary mirror. This technique and the dilution fridge concept will be used in future scan--modulated space instrument like the ESA Planck mission project.Comment: 10 pages, LaTeX, 12 figures, accepted for publication in Astronomy and Astrophysics Supplement Serie

A unique bacteriohopanetetrol stereoisomer of marine anammox

Anaerobic ammonium oxidation (anammox) is a major process of bioavailable nitrogen removal from marine systems. Previously, a bacteriohopanetetrol (BHT) isomer, with unknown stereochemistry, eluting later than BHT using high performance liquid chromatography (HPLC), was detected in ‘Ca. Scalindua profunda’ and proposed as a biomarker for anammox in marine paleo-environments. However, the utility of this BHT isomer as an anammox biomarker is hindered by the fact that four other, non-anammox bacteria are also known to produce a late-eluting BHT stereoisomer. The stereochemistry in Acetobacter pasteurianus, Komagataeibacter xylinus and Frankia sp. was known to be 17β, 21β(H), 22R, 32R, 33R, 34R (BHT-34R). The stereochemistry of the late-eluting BHT in Methylocella palustris was unknown. To determine if marine anammox bacteria produce a unique BHT isomer, we studied the BHT distributions and stereochemistry of known BHT isomer producers and of previously unscreened marine (‘Ca. Scalindua brodeae’) and freshwater (‘Ca. Brocadia sp.’) anammox bacteria using HPLC and gas chromatographic (GC) analysis of acetylated BHTs and ultra high performance liquid chromatography (UHPLC)-high resolution mass spectrometry (HRMS) analysis of non-acetylated BHTs. The 34R stereochemistry was confirmed for the BHT isomers in Ca. Brocadia sp. and Methylocella palustris. However, ‘Ca. Scalindua sp.’ synthesise a stereochemically distinct BHT isomer, with still unconfirmed stereochemistry (BHT-x). Only GC analysis of acetylated BHT and UHPLC analysis of non-acetylated BHT distinguished between late-eluting BHT isomers. Acetylated BHT-x and BHT-34R co-elute by HPLC. As BHT-x is currently only known to be produced by ‘Ca. Scalindua spp.’, it may be a biomarker for marine anammox

PILOT: a balloon-borne experiment to measure the polarized FIR emission of dust grains in the interstellar medium

Future cosmology space missions will concentrate on measuring the polarization of the Cosmic Microwave Background, which potentially carries invaluable information about the earliest phases of the evolution of our universe. Such ambitious projects will ultimately be limited by the sensitivity of the instrument and by the accuracy at which polarized foreground emission from our own Galaxy can be subtracted out. We present the PILOT balloon project which will aim at characterizing one of these foreground sources, the polarization of the dust continuum emission in the diffuse interstellar medium. The PILOT experiment will also constitute a test-bed for using multiplexed bolometer arrays for polarization measurements. We present the results of ground tests obtained just before the first flight of the instrument.Comment: 17 pages, 13 figures. Presented at SPIE, Millimeter, Submillimeter, and Far-Infrared Detectors and Instrumentation for Astronomy VII. To be published in Proc. SPIE volume 915

Code-level model checking in the software development workflow at Amazon Web Services

This article describes a style of applying symbolic model checking developed over the course of four years at Amazon Web Services (AWS). Lessons learned are drawn from proving properties of numerous C‐based systems, for example, custom hypervisors, encryption code, boot loaders, and an IoT operating system. Using our methodology, we find that we can prove the correctness of industrial low‐level C‐based systems with reasonable effort and predictability. Furthermore, AWS developers are increasingly writing their own formal specifications. As part of this effort, we have developed a CI system that allows integration of the proofs into standard development workflows and extended the proof tools to provide better feedback to users. All proofs discussed in this article are publicly available on GitHub

Seasonal paleoecological records from antler collagen δ13C and δ15N

Cervids living in high latitudes have evolved to thrive in ecosystems that experience dramatic seasonal changes. Understanding these seasonal adaptations is important for reconstructing cervid life histories, ecosystem dynamics and responses in the distant and not-so-distant past to changing seasonality caused by climate change. Cervid antlers provide a rare opportunity for insight into faunal seasonal ecology, as they are grown and shed each year. Stable isotopes of carbon and nitrogen measured directly from antlers have the potential to provide seasonal dietary data for individuals. If the isotopic signals in bone and antler are controlled by the same metabolic processes, then the stable carbon and nitrogen isotope compositions of collagen (δ13CColl and δ15NColl) from incrementally grown antler tissue provide time-constrained dietary signals from the spring and summer growth season. Bone, by comparison, provides an average signal over several years. The amino acid (glutamate and phenylalanine) δ15N in antlers from modern captive caribou showed similar trophic discrimination factors to earlier results for other collagenous tissues (bone, tooth dentin and cementum). Hence, growth rate was not the primary control on the stable isotope composition of antler collagen. We applied this knowledge to assess seasonal shifts in Quaternary fossils of three Cervidae species: elk (Cervus elaphus), moose (Alces alces), and caribou (Rangifer tarandus). Paired antler-bone δ13CColl and δ15NColl from the same individual were used to identify differences between summer and annual diet and ecology. Intra-antler isotopic variability from serially sampled antlers was used to examine seasonal dietary shifts and specialization

Reconstructing the reproductive mode of an Ediacaran macro-organism.

Enigmatic macrofossils of late Ediacaran age (580-541 million years ago) provide the oldest known record of diverse complex organisms on Earth, lying between the microbially dominated ecosystems of the Proterozoic and the Cambrian emergence of the modern biosphere. Among the oldest and most enigmatic of these macrofossils are the Rangeomorpha, a group characterized by modular, self-similar branching and a sessile benthic habit. Localized occurrences of large in situ fossilized rangeomorph populations allow fundamental aspects of their biology to be resolved using spatial point process techniques. Here we use such techniques to identify recurrent clustering patterns in the rangeomorph Fractofusus, revealing a complex life history of multigenerational, stolon-like asexual reproduction, interspersed with dispersal by waterborne propagules. Ecologically, such a habit would have allowed both for the rapid colonization of a localized area and for transport to new, previously uncolonized areas. The capacity of Fractofusus to derive adult morphology by two distinct reproductive modes documents the sophistication of its underlying developmental biology.This work has been supported by the Natural Environment Research Council [grant numbers NE/I005927/1 to C.G.K., NE/J5000045/1 to J.J.M., NE/L011409/1 to A.G.L. and NE/G523539/1 to E.G.M.], and a Henslow Junior Research Fellowship from Cambridge Philosophical Society to A.G.L.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/nature1464