Multi-disciplinary medical case study development for first year medical students

This poster will describe the history of the medical informatics course and the process of designing the case studies to fit into the new course management system, and will review the experiences of the librarians involved

Developing and Sustaining a Web-Based Library Newsletter

Before the rising popularity of the Internet in the mid 1990s, Scott Memorial Library, like most others, used printed newsletters to inform users about new resources and services. The JEFFLINE Forum, our attempt to resuscitate the Library\u27s newsletter by employing the technical advantages-and increasing presence-of the Web, debuted in October of 1999. The fifth anniversary of the Forum seemed like a good time to look back at its evolution and to examine some of the challenges inherent in developing and sustaining a library newsletter

Perioperative Risk Factors for Postoperative Delirium in Patients Undergoing Esophagectomy

Background Postoperative delirium affects up to 50% of patients undergoing esophagectomy and is associated with negative outcomes. The perioperative risk factors for delirium in this population are not well understood. We conducted this study to assess perioperative risk factors for postoperative delirium among esophagectomy patients. Methods We performed a secondary data analysis of patients enrolled in a randomized controlled trial evaluating the efficacy of haloperidol prophylaxis postoperatively in reducing delirium among esophagectomy patients. Postoperative delirium was assessed twice daily using the Confusion Assessment Method for the ICU. Univariate and logistic regression analyses were performed to examine the association between perioperative variables and development of postoperative delirium. Results Of 84 consecutive esophagectomy patients, 27 (32%) developed postoperative delirium. Patients who developed postoperative delirium had higher APACHE II scores [22.1 (6.5) versus 17.4 (6.8); p=0.003], longer mechanical ventilation days [1.7 (1.4) versus 1.0 (1.1); p=0.001], and longer ICU days [5.1 (2.6) versus 2.6 (1.6); p<0.001]. In a logistic regression model, only ICU length of stay was found to have significant association with postoperative delirium [OR 1.65; 95% CI 1.21-2.25]. Conclusions ICU length of stay was significantly associated with postoperative delirium. Other perioperative factors including duration of surgery, blood loss, and hemoglobin levels were not significantly associated with postoperative delirium

Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP)

Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use. It is known and regularly demonstrated that praziquantel does not always eliminate an infection so in spite of the successes of control programs a residual of the reservoir survives to re-infect snails. The issue of diagnostic sensitivity becomes more critical in the assessment of program effectiveness. While serology, such as antigen capture tests might improve sensitivity, it has been shown that the presence of species-specific DNA fragments will indicate, most effectively, the presence of active parasites. Polymerase chain reaction (PCR) can amplify and detect DNA from urine residue captured on Whatman No. 3 filter paper that is dried after filtration. Previously we have detected S. mansoni and S. haematobium parasite-specific small repeat DNA fragment from filtered urine on filter paper by PCR. In the current study, we assessed the efficacy of detection of 86 urine samples for either or both schistosome parasites by PCR and loop-mediated isothermal amplification (LAMP) that were collected from a low to moderate transmission area in Ghana. Two different DNA extraction methods, standard extraction kit and field usable LAMP-PURE kit were also evaluated by PCR and LAMP amplification. With S. haematobium LAMP amplification for both extractions showed similar sensitivity and specificity when compared with PCR amplification (100%) verified by gel electrophoresis. For S. mansoni sensitivity was highest for LAMP amplification (100%) for standard extraction than PCR and LAMP with LAMP-PURE (99% and 94%). The LAMP-PURE extraction produced false negatives, which require further investigation for this field usable extraction kit. Overall high positive and negative predictive values (90% − 100%) for both species demonstrated a highly robust approach. The LAMP approach is close to point of care use and equally sensitive and specific to detection of species-specific DNA by PCR. LAMP can be an effective means to detect low intensity infection due to its simplicity and minimal DNA extraction requirement. This will enhance the effectiveness of surveillance and MDA control programs of schistosomiasis

Variation between some apricot varieties in regard to flowering phenology in Boldogkôváralja, Hungary

The aim of this study was the estimation of blossoming of 14 apricot cultivars in Boldogkôváralja in 2009, 2010 and 2011 seasons. And this will help growers to select appropriate varieties to their weather conditions. For this target the blooming period of 19 apricot varieties of different origin was observed in three subsequent years. There was no large difference in the beginning of blooming in the different years, and the greatest variation between the start date of flowering was about 1 to 3 days as the place of experiment site near to northern border and also, length of flowering period of apricot trees is also inversely related to date when blooming started. The little differences in flowering dates and flowering periods due to the high temperature through the three seasons of study

Storability of some apricot varieties as affected by storage period

The aim of this study was the estimation of storability of 10 apricot varieties in regard to percentage fruit weight loss, firmness,acidity and T.S.S in fruit during storage periods from one week to 4 weeks. All the variety gave the same trend as all of them loss weight,firmness decreased, acidity and T.S.S decreased but the differences were not the same in all varieties. in case of some varieties the percentageof fruit weight loss reached to about 9 % after 28 days also the differences between varieties in two seasons refer that this character isdetermined by genetic factors beside effect of environmental and agriculture factors

Variation between some apricot varieties in regard to flowering phenology in Boldogkôváralja, Hungary

The aim of this study was the estimation of blossoming of 14 apricot cultivars in Boldogkôváralja in 2009, 2010 and 2011 seasons.And this will help growers to select appropriate varieties to their weather conditions. For this target the blooming period of 19 apricot varietiesof different origin was observed in three subsequent years. There was no large difference in the beginning of blooming in the different years,and the greatest variation between the start date of flowering was about 1 to 3 days as the place of experiment site near to northern border andalso, length of flowering period of apricot trees is also inversely related to date when blooming started. The little differences in flowering datesand flowering periods due to the high temperature through the three seasons of study

Storability of some apricot varieties as affected by storage period

The aim of this study was the estimation of storability of 10 apricot varieties in regard to percentage fruit weight loss, firmness, acidity and T.S.S in fruit during storage periods from one week to 4 weeks. All the variety gave the same trend as all of them loss weight, firmness decreased, acidity and T.S.S decreased but the differences were not the same in all varieties. in case of some varieties the percentage of fruit weight loss reached to about 9 % after 28 days also the differences between varieties in two seasons refer that this character is determined by genetic factors beside effect of environmental and agriculture factors

Simple and Rapid Enzymatic Method for the Synthesis of Single-Strand Oligonucleotides Containing Trifluorothymidine

To investigate the mechanism of trifluorothymidine (TFT)-induced DNA damage, we developed an enzymatic method for the synthesis of single-strand oligonucleotides containing TFT-monophosphate residues. Sixteen-mer oligonucleotides and 14-mer 5′-phosphorylated oligonucleotides were annealed to the template of 25-mer, so as to empty one nucleotide site. TFT-triphosphate was incorporated into the site by DNA polymerase and then ligated to 5′-phosphorylated oligonucleotides by DNA ligase. The synthesized 31-mer oligonucleotides containing TFT residues were isolated from the 25-mer complementary template by denaturing polyacrylamide electrophoresis. Using these single-strand oligonucleotides containing TFT residues, the cleavage of TFT residues from DNA, using mismatch uracil-DNA glycosylase (MUG) of E.coli origin, was compared with that of 5-fluorouracil (5FU) and 5-bromodeoxyuridine (BrdU). The TFT/A pair was not cleaved by MUG, while the other pairs, namely, 5FU/A, 5FU/G, BrdU/A, BrdU/G, and TFT/G, were easily cleaved from each synthesized DNA. Thus, this method is useful for obtaining some site-specifically modified oligonucleotides

Volcanic fertilization of Balinese rice paddies

Abstract Since the advent of high-yielding &apos;&apos;Green Revolution&apos;&apos; rice agriculture in the 1970s, Balinese farmers have been advised to supply all the potassium and phosphate needed by rice plants via chemical fertilizers. This policy neglects the contribution of minerals leached from the volcanic soil and transported via irrigation systems. We documented frequent deposition of volcanic ash deposits to rice producing watersheds. Concentrations of phosphorus in rivers were between 1 and 4 mg l − 1 PO 4 , increasing downstream. We measured extractable potassium and phosphate levels in the soils of unfertilized Balinese rice paddies, and found them to be indistinguishable from those in fertilized paddies, and sufficient for high grain yields. Field experiments varying phosphorus applications to rice fields from 0 to 100 kg superphosphate per hectare (7-26 kg P ha − 1 ) demonstrated small increases in harvest yields only with the smallest additions. Direct measurements of PO 4 in irrigation waters indicate that most of the added phosphate flows out of the paddies and into the river systems, accumulating to very high levels before reaching the coast