A planned program in estimation in addition

Thesis (M.A.)--Boston Universit

Revisión de la distribución de pequeños cetaceos frente al Perú

Review of the distribution of small cetaceans off Peru. The little information available on the occurrence of small cetaceans in Peruvian marine waters is reviewed. Data obtained between 1982 and 1987 by port monitoring, surveys of beaches and fish dumps, and sightings are included.Eighteen species of small cetaceans, belonging to four families, are presently known to occur at least occasionally within the 200 nautical miles limit of Peruvian marine waters; six species (markes with asterisk) represent new records for the study area. The species are: Phocoens spinipinnis, Tursiops truncatus, Grampus griseus; Lagenorhynchus obscurus; Delphinus delphis; Stenella attenuate; S. longirostris; S. coerulealba; Peponocephala electra (*); Feresa attenuate (*); Globicephala melaena; G. macrorhynchus (*); Pseudorca crassidens; Orcinus orca; Kogia breviceps; K. simus (*); Ziphius cavirostris (*); Mesoploson sp. (*)

Ultraplex- A rapid, flexible, all-in-one fastq demultiplexer [version 1; peer review- 1 approved]

BACKGROUND: The first step of virtually all next generation sequencing analysis involves the splitting of the raw sequencing data into separate files using sample-specific barcodes, a process known as “demultiplexing”. However, we found that existing software for this purpose was either too inflexible or too computationally intensive for fast, streamlined processing of raw, single end fastq files containing combinatorial barcodes. RESULTS: Here, we introduce a fast and uniquely flexible demultiplexer, named Ultraplex, which splits a raw FASTQ file containing barcodes either at a single end or at both 5’ and 3’ ends of reads, trims the sequencing adaptors and low-quality bases, and moves unique molecular identifiers (UMIs) into the read header, allowing subsequent removal of PCR duplicates. Ultraplex is able to perform such single or combinatorial demultiplexing on both single- and paired-end sequencing data, and can process an entire Illumina HiSeq lane, consisting of nearly 500 million reads, in less than 20 minutes. CONCLUSIONS: Ultraplex greatly reduces computational burden and pipeline complexity for the demultiplexing of complex sequencing libraries, such as those produced by various CLIP and ribosome profiling protocols, and is also very user friendly, enabling streamlined, robust data processing. Ultraplex is available on PyPi and Conda and via Github

Factors associated with good self-management in older adults with a schizophrenic disorder compared with older adults with physical illnesses

The number of older people living with a schizophrenic disorder (SD) is increasing yet little attention paid has been paid to the needs of this population relative to people with other chronic illnesses. In order to achieve optimal functioning people with a SD need to manage their illness and its impact; therefore, this study set out to determine the factors associated with self-management in this population. The illness management of people over 50 years of age and living with schizophrenia (n= 84) was compared with their peers who were diagnosed with a chronic physical illness (n= 216). Participants completed a survey that included an illness management inventory, self-rated health and sense of coherence. The results demonstrated that participants with a SD had lower illness management levels, particularly for understanding their symptoms and taking appropriate actions in relation to health care. Poor self-rated health and the presence of comorbid conditions had a pervasive negative effect on self-management factors in the SD group, whereas being married, having a greater sense of coherence and being voluntary to treatment had a positive effect. Nurses need to develop strategies to address general health and self-management in older adults living with a SD. © 2011 Blackwell Publishing

Understanding the Ecohydrology of Shallow, Drained and Marginal Blanket Peatlands

Peatlands are unique and important landscape systems, providing valuable ecosystem services such as water and carbon storage, water supply and flood attenuation. They are known to account for more than 10% of the world’s terrestrial carbon store and represent 50 – 70% of the global wetland resource. The UK government’s decision to support the IUCN, UK Peatland Program Commission of Inquiry on Peatlands, recognises the importance and urgency with which action is needed to understand and restore damaged peatland landscapes, and their associated ecosystem services. To meet this need, it is recognised that peatlands in the South West of the UK are important as bio-climatically and functionally marginal peatlands that are undergoing extensive restoration to reinstate key ecological and hydrological function. This thesis aims to improve understanding of the temporal and spatial variability of the ecohydrological structure and function of peatland ecosystems in the South West UK, and will provide the first baseline for the spatially distributed extrapolation of change across larger landscape extents. The research seeks to characterise the structure and function of peatland ecohydrology across multiple spatial and temporal scales. This is accomplished by bringing together remote sensing analyses of ecohydrological structure and function coupled with an integrated and high resolution hydrological monitoring system to characterise the spatial and temporal variability of runoff production and water storage across two headwater catchments. Key outcomes of this research are: 1. The development of novel methods to assess the spatial distribution of near surface hydrology in upland ecosystems using airborne thermal imaging data, 2. Improved understanding of how laser altimetry data can be used to measure the ecohydrology of landscapes more appropriately. 3. An empirical understanding of both the spatial and temporal variability of hydrology across representative sites within the moorlands of the South West UK. The high-resolution monitoring data are the first to describe the hydrological processes operating in these peatlands systems effectively, and provide an insight into how these processes are controlled by the anthropogenic drainage networks that are present throughout this shallow marginal peatland system.South West Wate

Quantum point contact due to Fermi-level pinning and doping profiles in semiconductor nanocolumns

We show that nanoscale doping profiles inside a nanocolumn in combination with Fermi-level pinning at the surface give rise to the formation of a saddle-point in the potential profile. Consequently, the lateral confinement inside the channel varies along the transport direction, yielding an embedded quantum point contact. An analytical estimation of the quantization energies will be given

Implementing medical revalidation in the United Kingdom: Findings about organisational changes and impacts from a survey of Responsible Officers.

Objective To describe the implementation of medical revalidation in healthcare organisations in the United Kingdom and to examine reported changes and impacts on the quality of care. Design A cross-sectional online survey gathering both quantitative and qualitative data about structures and processes for medical revalidation and wider quality management in the organisations which employ or contract with doctors (termed 'designated bodies') from the senior doctor in each organisation with statutory responsibility for medical revalidation (termed the 'Responsible Officer'). Setting United Kingdom Participants Responsible Officers in designated bodies in the United Kingdom. Five hundred and ninety-five survey invitations were sent and 374 completed surveys were returned (63%). Main outcome measures The role of Responsible Officers, the development of organisational mechanisms for quality assurance or improvement, decision-making on revalidation recommendations, impact of revalidation and mechanisms for quality assurance or improvement on clinical practice and suggested improvements to revalidation arrangements. Results Responsible Officers report that revalidation has had some impacts on the way medical performance is assured and improved, particularly strengthening appraisal and oversight of quality within organisations and having some impact on clinical practice. They suggest changes to make revalidation less 'one size fits all' and more responsive to individual, organisational and professional contexts. Conclusions Revalidation appears primarily to have improved systems for quality improvement and the management of poor performance to date. There is more to be done to ensure it produces wider benefits, particularly in relation to doctors who already perform well

Contralateral Total Hip Arthroplasty After Hindquarter Amputation

We describe the management and outcome of a 62-year old lady who developed severe osteoarthritis of the hip, nine years after a hindquarter amputation for radiation-induced sarcoma of the contralateral pelvis. The difficulties of stabilising the pelvis intraoperatively and the problems of postoperative rehabilitation are outlined. The operation successfully relieved her pain and restored limited mobility

The differential absorption of a series of P-glycoprotein substrates in isolated perfused lungs from Mdr1a/1b genetic knockout mice can be attributed to distinct physico-chemical properties: an insight into predicting transporter-mediated, pulmonary specific disposition

Purpose To examine if pulmonary P-glycoprotein (P-gp) is functional in an intact lung; impeding the pulmonary absorption and increasing lung retention of P-gp substrates administered into the airways. Using calculated physico-chemical properties alone build a predictive Quantitative Structure-Activity Relationship (QSAR) model distinguishing whether a substrate’s pulmonary absorption would be limited by P-gp or not. Methods A panel of 18 P-gp substrates were administered into the airways of an isolated perfused mouse lung (IPML) model derived from Mdr1a/Mdr1b knockout mice. Parallel intestinal absorption studies were performed. Substrate physico-chemical profiling was undertaken. Using multivariate analysis a QSAR model was established. Results A subset of P-gp substrates (10/18) displayed pulmonary kinetics influenced by lung P-gp. These substrates possessed distinct physico-chemical properties to those P-gp substrates unaffected by P-gp (8/18). Differential outcomes were not related to different intrinsic P-gp transporter kinetics. In the lung, in contrast to intestine, a higher degree of non-polar character is required of a P-gp substrate before the net effects of efflux become evident. The QSAR predictive model was applied to 129 substrates including eight marketed inhaled drugs, all these inhaled drugs were predicted to display P-gp dependent pulmonary disposition. Conclusions Lung P-gp can affect the pulmonary kinetics of a subset of P-gp substrates. Physico-chemical relationships determining the significance of P-gp to absorption in the lung are different to those operative in the intestine. Our QSAR framework may assist profiling of inhaled drug discovery candidates that are also P-gp substrates. The potential for P-gp mediated pulmonary disposition exists in the clinic