Do giant barnacles contribute to deep-water biogenic reef formation in Maltese waters?

Deep-sea ROV surveys in the west Malta Graben allowed re-evaluation of previous work on the association of the giant barnacle, Pachylasma giganteum, with cold-water corals and other habitat-forming species, and this species’ contribution to formation of biogenic structures. While only a minor contributor to habitat architecture when anthozoans and other large sessile species are dominant, P. giganteum may become a habitat-former in the absence of competing species.peer-reviewe

A rapid review of sexual wellbeing definitions and measures: should we now include sexual wellbeing freedom?

An increasing number of studies refer to sexual wellbeing and/or seek to measure it, and the term appears across various policy documents, including sexual health frameworks in the UK. We conducted a rapid review to determine how sexual wellbeing has been defined, qualitatively explored and quantitatively measured. Eligible studies selected for inclusion from OVID Medline, PsychInfo, PubMed, Embase, CINAHL were: in English language, published after 2007, were peer-reviewed full articles, focused on sexual wellbeing (or proxies for, e.g. satisfaction, function), and quantitatively or qualitatively assessed sexual wellbeing. We included studies with participants aged 16–65. Given study heterogeneity, our synthesis and findings are reported using a narrative approach. We identified 162 papers, of which 10 offered a definition of sexual wellbeing. Drawing upon a socio-ecological model, we categorised the 59 dimensions we identified from studies under three main domains: cognitive-affect (31 dimensions); inter-personal (22 dimensions); and socio-cultural (6 dimensions). Only 11 papers were categorised under the socio-cultural domain, commonly focusing on gender inequalities or stigma. We discuss the importance of conceptualising sexual wellbeing as individually experienced but socially and structurally influenced, including assessing sexual wellbeing freedom: a person’s freedom to achieve sexual wellbeing, or their real opportunities and liberties

PCR-Independent Detection of Bacterial Species-Specific 16S rRNA at 10 fM by a Pore-Blockage Sensor.

A PCR-free, optics-free device is used for the detection of Escherichia coli (E. coli) 16S rRNA at 10 fM, which corresponds to ~100-1000 colony forming units/mL (CFU/mL) depending on cellular rRNA levels. The development of a rapid, sensitive, and cost-effective nucleic acid detection platform is sought for the detection of pathogenic microbes in food, water and body fluids. Since 16S rRNA sequences are species specific and are present at high copy number in viable cells, these nucleic acids offer an attractive target for microbial pathogen detection schemes. Here, target 16S rRNA of E. coli at 10 fM concentration was detected against a total RNA background using a conceptually simple approach based on electromechanical signal transduction, whereby a step change reduction in ionic current through a pore indicates blockage by an electrophoretically mobilized bead-peptide nucleic acid probe conjugate hybridized to target nucleic acid. We investigated the concentration detection limit for bacterial species-specific 16S rRNA at 1 pM to 1 fM and found a limit of detection of 10 fM for our device, which is consistent with our previous finding with single-stranded DNA of similar length. In addition, no false positive responses were obtained with control RNA and no false negatives with target 16S rRNA present down to the limit of detection (LOD) of 10 fM. Thus, this detection scheme shows promise for integration into portable, low-cost systems for rapid detection of pathogenic microbes in food, water and body fluids

Compilation of an interpretation manual for marine habitats within the 25 NM Fisheries Management Zone around the Republic of Malta

Several EU Directives and regional conventions are concerned with the protection and conservation of marine habitats of special importance; a necessary prerequisite to implement such legislation are marine habitat classification systems and information on the distribution of marine habitat types. There are several habitat classification schemes in use for the description, designation and monitoring of habitats. The ‘Interpretation Manual for Marine Habitats within the 25 NM Fisheries Management Zone around the Republic of Malta’ provides an updated description of marine habitats found around the Maltese Islands, as well as a cross-referencing scheme for the different habitat classification systems. Marine habitats are listed according to bathymetric zone, going from shallow to deep water. The bathymetric zones included in the interpretation manual are: (1) mediolittoral, (2) infralittoral, (3) circalittoral, and (4) bathyal. Habitats listed in the European Nature Information System (EUNIS) database were reviewed, habitats which are present in the Maltese Islands were identified, and ‘new’ habitat categories have been included in the manual where there was no equivalent EUNIS habitat. For each habitat category presented, cross- references to the system used in connection with the Protocol for Specially Protected Areas and Biodiversity in the Mediterranean (SPABM) of the Barcelona Convention, to the Marine Strategy Framework Directive (MSFD) classification system of predominant habitat types, as well as to the marine habitats listed in Annex I of the ‘Habitats Directive’, were established. In addition a scheme to enable the assessment of the conservation status of marine habitats as required under the ‘Habitats Directive’ was drawn up by considering the habitat range, the area occupied by a habitat, habitat structure and function, and the habitat’s future prospects. Suitable indicators to assess favourable conservation status are presented for (i) sandbanks, (ii) Posidonia beds, (iii) reefs and (iv) sea caves. In addition, indicators to assess the conservation status of maerl beds are presented due to the local significance and the sensitivity of this habitat. The manual thus intends to aid in the implementation of measures for the protection of marine habitats and hence, ultimately, to ensure effective and efficient management of the marine environment under the jurisdiction of Malta up to the 25 NM boundary, in line with the relevant legislation.peer-reviewe

Relational Social Justice Identity Development among Chinese International High School Students

This research explored the development of a social justice orientation and identity in a sample of high school students in Hong Kong. A sample of 12 students, four teachers, the Principal, and the School Counselor were interviewed in depth about the roles of community service, service learning, and social justice in their identities and school activities. Grounded theory was used to analyze the interview data and six main themes and several subcategories emerged: Six themes emerged from the interview data: (1) Community service as a social experience; (2) Service as a personally rewarding experience; (3) Appreciation of cultural differences; (4) Self-awareness and selflessness; (5) Adult support for social justice; and (6) Challenges in developing a social justice agenda. Implications for social justice identity development in counseling and education are discussed

Boys II Men: A Culturally-Responsive School Counseling Group for Urban High School Boys of Color

Using a participatory and collaborative approach, we developed, implemented, and evaluated a culturally responsive school counseling group, Boys II Men, for 11 low-income diverse male students of color at an urban public school. The content of the group focused on five areas: social connections and support, exploring gender roles, navigating identities, school engagement, and future planning. We worked closely with teachers, school staff, and counselors to foster a supportive and positive school climate (Beesley, 2004). Each student was interviewed about his experience in the group to assess the impact of the strategies and techniques used. We also analyzed the specific content of each module for main themes. Strengths and weaknesses of the group were also assessed at post-test. Innovative methods and practical applications for school counselors are discussed

Maximising MEDITS: using data collected from trawl surveys to characterise the benthic and demersal assemblages of the circalittoral and deeper waters around the Maltese Islands

Data from MEDITS trawl surveys in the waters surrounding the Maltese Islands were analysed to characterise the benthic and demersal assemblages of the central Mediterranean, which are only poorly known, hampering Maltese authorities in meeting legislative obligations. A total of 147 benthic species and 98 demersal species were identified. These formed four well-defined assemblages, whose structure and composition were related to depth. Total species richness and abundance of both demersal and benthic species decreased with depth. The most abundant structuring epibenthic species which characterised the identified assemblages were the tall sea pen Funicularia quadrangularis (present at depths of ca. 50–700 m), the red sea pen Pennatula rubra (ca. 100–450 m), the hydroids Lytocarpia myriophyllum (ca. 100–250 m) and Nemertesia sp. (ca. 100–600), the soft coral Alcyonium palmatum (ca. 100–400 m), and the anemone Actinauge richardi (ca. 100–600 m). These results illustrate how, in the absence of dedicated benthic mapping studies, MEDITS data can be used to provide preliminary information that would not otherwise be available.peer-reviewe

Vegetation plot and trait data from phonolitic and basaltic rocks on La Palma (Canary Islands, Spain)

Geodiversity promotes biodiversity by increasing habitat heterogeneity. In times of a global biodiversity decline, data about diversity on such geological elements gains importance, also regarding conservation and restoration. In the Canary Islands, phonolitic rocks are geological elements of volcanic origin that represent additional habitat for species. In the southern part of the island La Palma, phonolite rocks can be encountered in matrices of young lava. We provide biotic and abiotic records from 60 plots of 2 m × 2 m, sampled on phonolitic and neighbouring basaltic outcrops at four different sites that cover a wide environmental gradient. The recorded parameters were species abundances (percentage cover), plant individuals' frequency (number of plant individuals per plot and species), plant growth height (in cm), and canopy diameter (in cm). Additionally, lichen cover (percentage cover) was estimated. To describe site specific characteristics, we recorded plot surface dynamics (3D rock surface), aspect, and inclination. This data set covers vegetation and trait data comparing phonolites and basalts on La Palma. It can be used for island biogeography, vegetation ecology and conservation sciences to help support fundamental research related to insular biodiversity and endemism, and to identify priority areas for protection and preservation in the Canary Islands

Workflows in bioinformatics: meta-analysis and prototype implementation of a workflow generator

BACKGROUND: Computational methods for problem solving need to interleave information access and algorithm execution in a problem-specific workflow. The structures of these workflows are defined by a scaffold of syntactic, semantic and algebraic objects capable of representing them. Despite the proliferation of GUIs (Graphic User Interfaces) in bioinformatics, only some of them provide workflow capabilities; surprisingly, no meta-analysis of workflow operators and components in bioinformatics has been reported. RESULTS: We present a set of syntactic components and algebraic operators capable of representing analytical workflows in bioinformatics. Iteration, recursion, the use of conditional statements, and management of suspend/resume tasks have traditionally been implemented on an ad hoc basis and hard-coded; by having these operators properly defined it is possible to use and parameterize them as generic re-usable components. To illustrate how these operations can be orchestrated, we present GPIPE, a prototype graphic pipeline generator for PISE that allows the definition of a pipeline, parameterization of its component methods, and storage of metadata in XML formats. This implementation goes beyond the macro capacities currently in PISE. As the entire analysis protocol is defined in XML, a complete bioinformatic experiment (linked sets of methods, parameters and results) can be reproduced or shared among users. Availability: (interactive), (download). CONCLUSION: From our meta-analysis we have identified syntactic structures and algebraic operators common to many workflows in bioinformatics. The workflow components and algebraic operators can be assimilated into re-usable software components. GPIPE, a prototype implementation of this framework, provides a GUI builder to facilitate the generation of workflows and integration of heterogeneous analytical tools