The Role of Perceived Uncertainty, Ego Identity, and Perceived Behavioral Control in Predicting Patient's Attitude Toward Medical Surgery

Medical surgery has sometimes become the only best choice for a patient's well-being. Unfortunately, not all patients have the willingness to live it. Often, therapeutic failure is caused by uncooperative attitudes of the patients which originate from their negative attitudes toward the surgery. This research is aimed at finding a theoretical model to explain psychological factors forming the patient's attitudes. This predictive correlational research was conducted on 99 patients suffering heart disease and cancer continuum who require medical surgery in DKI Jakarta, Indonesia. Research results showed that a commitment aspect of ego identity is able to indirectly predict attitude toward medical surgery through mediation of perceived uncertainty. Perceived behavioral control directly predicts the attitude in a negative direction. This research concludes that patients' commitment towards their identity plays a significant role as they deal with medical surgery

Efek Aplikasi Topikal Gel Ekstrak Pandan Wangi terhadap Penyembuhan Luka Gingiva

The Effect of topical application pandan extract gel on gingival wound. Post-gingivectomy wound is usually covered by periodontal dressing, which generally contains some chemical compounds to protect the wound. However, it can provide allergic effect on some patients. Pandan leaves (Pandanus amaryllifolius Roxb.) contain a number of active substances that have anti-inflammatory, antioxidant and antibacterial effect and play a role in wound healing. This study aims to determine the effect of topical application of Pandan leaf extract gel on gingival wound healing. Gingivectomy model was carried out on mandibular incisive gingival using the 2.5-mm punch biopsy. Thirty-six rats were randomly divided into 3 groups of treatment: negative control (CMC-Na), positive control (Aloclair), and 50% of pandan extract gel. The gels on each group were applied (twice in a day) to the wound area after gingivectomy. The observation of the wound healing process was also carried out on day 1, 3, 7, and 14 by making the histological preparations of gingival wound area. The number of blood vessels was observed using microscope and data was analysed using Two- Way Anova and LSD. The result showed that number of blood vessel increased on day 3 and the peak was on day 7. Anova and LSD test showed several significant differences comparison the number blood vessel between treatment and control. In conclusion, topical application Pandan leaves extract gel could accelerate gingival wound healing

SOSIALISASI BANK SAMPAH DI KELURAHAN PANDAN KASTURI

Pola hidup sehat merupakan aspek yang sangat penting dalam kehidupan manusia apalagi didalam masa pandemi covid-19 ini. Salah satu cara penerapan pola hidup sehat adalah dengan menjaga kebersihan lingkungan. Sampah tentunya menjadi acuan besar dalam mempengaruhi lingkungan, untuk itu pentingnya pengelolahan sampah sangat di perlukan untuk masyarakat. Tujuan PKM ini adalah untuk memberikan pemahaman tentang pentingnya pengelolahan sampah dengan adanya bank sampah. Kegiatan ini melibatkan ketua-ketua RT/RW yang berada Kelurahan Pandan Kasturi, Kecamatan Sirimau,Kota Ambon dan juga Team Green Mollucas Ambon sebagai narasumber. Metode yang digunakan dalam kegiatan ini adalah Ceramah dan Diskusi. Ceramah yang diberikan dalam bentuk penjelasan powerpoint oleh team Green Mollucas Ambon dan dilanjutkan dengan sesi diskusi atau tanya jawab. Hasil dari kegiatan ini adalah Ketua-Ketua RT/RW Kelurahan Pandan Kasturi ,Kecamatan Sirimau,Kota Ambon dapat menerima pengetahuan tentang penting pengelolah sampah dengan adanya Bank Sampah dan bahkan memiliki tekad untuk membangun bank sampah di Kelurahan Pandan Kasturi,Kecamatan Sirimau ,Kota Ambon serta meminta pendampingan lebih lanjut dari Green Moluccas mengenai pembangunan Bank Sampah

Looking to the future of zebrafish as a model to understand the genetic basis of eye disease

In this brief commentary, we provide some of our thoughts and opinions on the current and future use of zebrafish to model human eye disease, dissect pathological progression and advance in our understanding of the genetic bases of microphthalmia, andophthalmia and coloboma (MAC) in humans. We provide some background on eye formation in fish and conservation and divergence across vertebrates in this process, discuss different approaches for manipulating gene function and speculate on future research areas where we think research using fish may prove to be particularly effective

Histone Deacetylase Activity Modulates Alternative Splicing

There is increasing evidence to suggest that splicing decisions are largely made when the nascent RNA is still associated with chromatin. Here we demonstrate that activity of histone deacetylases (HDACs) influences splice site selection. Using splicing-sensitive microarrays, we identified ∼700 genes whose splicing was altered after HDAC inhibition. We provided evidence that HDAC inhibition induced histone H4 acetylation and increased RNA Polymerase II (Pol II) processivity along an alternatively spliced element. In addition, HDAC inhibition reduced co-transcriptional association of the splicing regulator SRp40 with the target fibronectin exon. We further showed that the depletion of HDAC1 had similar effect on fibronectin alternative splicing as global HDAC inhibition. Importantly, this effect was reversed upon expression of mouse HDAC1 but not a catalytically inactive mutant. These results provide a molecular insight into a complex modulation of splicing by HDACs and chromatin modifications

Phototoxicity in live fluorescence microscopy, and how to avoid it.

Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences are often underestimated. Damage to cellular macromolecules upon excitation light illumination can impair sample physiology, and even lead to sample death. In this review, we explain how phototoxicity influences live samples, and we highlight that, besides the obvious effects of phototoxicity, there are often subtler consequences of illumination that are imperceptible when only the morphology of samples is examined. Such less apparent manifestations of phototoxicity are equally problematic, and can change the conclusions drawn from an experiment. Thus, limiting phototoxicity is a prerequisite for obtaining reproducible quantitative data on biological processes. We present strategies to reduce phototoxicity, e.g. limiting the illumination to the focal plane and suggest controls for phototoxicity effects. Overall, we argue that phototoxicity needs increased attention from researchers when designing experiments, and when evaluating research findings

Cervical Spine Injury: Nature and Complication

BACKGROUND: Traumatic cervical spine injury is the commonest and most devastating injuries of the musculoskeletal system occurring more commonly in young patients from high energy vehicular accidents. This is particularly important considering the state of health care delivery system in Nigeria, increasing use of vehicles and belligerent flaunting of traffic rules. This study highlights the pattern of the injuries of the cervical spine as a distinct pathological entity which has not been documented before, the difficulties, the complications encountered and our attempt at overcoming these. METHODOLOGY: This is a three year prospective analysis of all patients with cervical spine injury. Treatment was conservative, Frankel's gradling was used as index of monitor, complications were documented and treated as they occurred and follow-up was for a minimum period of 12 months. RESULT: Twenty-eight patients were recruited comprising 25 (89.3%) males and three 910.7%) with a mean age of 30.5 (+5.54) years. Twenty five (89.3%) patients were aged 20.35 years. Road traffic accident was the leading cause in 22 (78.6%). Five (22.7%) patients of these had worsening of neurological status on transit to the hospital soon after the accidents. Ten (35.7%) patients had complete cord injury, 13(46.4%), incomplete and five had no cord injury at all. Twenty (71.4%) patients sustained associated injuries, facial lacerations and head injury being the commonest. Eleven (39.3%) patients had an improvement of at least one Frankel's grade during the course of rehabilitation. The commonest complications in our series were, urinary tract infection in 11 (39.3%); pressure ulcers in 11 (39.3%). Others were bronchopneumonia 9(32.1%); muscle spasms 9 (32.1%). Mortality was 7.1% both from respiratory failure. The mean duration of hospital stay was 95.4 (+78.5; range 0-2210 days. CONCLUSION: The outlook for the cervical injured patient remains bleak. More attention should be devoted to preventive measures backed by adequate and appropriate government legislation. The Federal Road Safety Corp should be empowered and their scope widened to increase their relevance in the community. A well run ambulance service scheme anchored by the ministry of health is long overdue and there is no better time than now to establish regional spinal center for adequate rehabilitation of the spinal injured patient. KEY WORDS: Traumatic cervical spine injury; Frankel's grade; urinary tract infection; bronchopneumonia, spinal center. Nigerian Journal of Orthopaedics and Trauma Vol.3(2) 2004: 149-16

Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina.

The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination