Whole blood NAD and NADP concentrations are not depressed in subjects with clinical pellagra

Population surveys for niacin deficiency are normally based on clinical signs or on biochemical measurements of urinary niacin metabolites. Status may also be determined by measurement of whole blood NAD and NADP concentrations. To compare these methods, whole blood samples and spot urine samples were collected from healthy subjects (n = 2) consuming a western diet, from patients (n = 34) diagnosed with pellagra and attending a pellagra clinic in Kuito (central Angola, where niacin deficiency is endemic), and from female community control subjects (n = 107) who had no clinical signs of pellagra. Whole blood NAD and NADP concentrations were measured by microtiter plate-based enzymatic assays and the niacin urinary metabolites 1-methyl-2-pyridone-5-carboxamide (2-PYR) and 1-methylnicotinamide (1-MN) by HPLC. In healthy volunteers, inter- and intra-day variations for NAD and NADP concentrations were much lower than for the urinary metabolites, suggesting a more stable measure of status. However, whole blood concentrations of NAD and NADP or the NAD:NADP ratio were not significantly depressed in clinical pellagra. In contrast, the concentrations of 2-PYR and 1-MN, expressed relative to either creatinine or osmolality, were lower in pellagra patients and markedly higher following treatment. The use of the combined cut-offs (2-PYR <3.0 micromol/mmol creatinine and 1-MN <1.3 micromol/mmol creatinine) gave a sensitivity of 91% and specificity of 72%. In conclusion, whole blood NAD and NADP concentrations gave an erroneously low estimate of niacin deficiency. In contrast, spot urine sample 2-PYR and 1-MN concentrations, relative to creatinine, were a sensitive and specific measure of deficiency

Low and deficient niacin status and pellagra are endemic in postwar Angola

BACKGROUND: Outbreaks of pellagra were documented during the civil war in Angola, but no contemporary data on the incidence of pellagra or the prevalence of niacin deficiency were available. OBJECTIVE: The objective was to investigate the incidence of pellagra and the prevalence of niacin deficiency in postwar Angola and their relation with dietary intake, poverty, and anthropometric status. DESIGN: Admissions data from 1999 to 2004 from the pellagra treatment clinic in Kuito, Angola, were analyzed. New patients admitted over 1 wk were examined, and urine and blood samples were collected. A multistage cluster population survey collected data on anthropometric measures, household dietary intakes, socioeconomic status, and clinical signs of pellagra for women and children. Urinary excretion of 1-methylnicotinamide, 1-methyl-2-pyridone-5-carboxymide, and creatinine was measured and hemoglobin concentrations were measured with a portable photometer. RESULTS: The incidence of clinical pellagra has not decreased since the end of the civil war in 2002. Low excretion of niacin metabolites was confirmed in 10 of 11 new clinic patients. Survey data were collected for 723 women aged 15-49 y and for 690 children aged 6-59 mo. Excretion of niacin metabolites was low in 29.4% of the women and 6.0% of the children, and the creatinine-adjusted concentrations were significantly lower in the women than in the children (P < 0.001, t test). In children, niacin status was positively correlated with the household consumption of peanuts (r = 0.374, P = 0.001) and eggs (r = 0.290, P = 0.012) but negatively correlated with socioeconomic status (r = -0.228, P = 0.037). CONCLUSIONS: The expected decrease in pellagra incidence after the end of the civil war has not occurred. The identification of niacin deficiency as a public health problem should refocus attention on this nutritional deficiency in Angola and other areas of Africa where maize is the staple

Is new olive farming sustainable? A spatial comparison of productive and environmental performances between traditional and new olive orchards with the model OliveCan

Olive (Olea europaea L.) is a widely spread tree species in the Mediterranean. In the last decades, olive farming has known major management changes with high economic and environmental impacts. The fast track expansion of this modern olive farming in these recent years casts doubts on the sustainability of such important tree plantation across the Mediterranean. In this work, we performed a spatial modelling analysis to investigate the implications of climate variability and farming management on the productivity and environmental performances of olive orchards around the Mediterranean. Implementation of this research is based on the use of OliveCan; a process-based model able to illustrate responses of water and carbon balances to weather variables, soil characteristics and management techniques enabling the comprehension of olive orchard dynamics under heterogeneous conditions of climate and agricultural practices. Four main intensification levels were adopted to reflect the main olive grove types from traditional to new intensive plantations: low density LD (100 trees ha−1), medium density MD (200 trees ha−1), high density HD (400 trees ha−1) and super high density SHD (1650 trees ha−1). Managements tested were intensification, water supply (rainfed, deficit and full irrigated) and the fate of pruning residues (exported or left on the soil). Two cases studies in two of the main Mediterranean olive-growing regions with contrasting environmental conditions, Tuscany and Jaen regions, focused on mitigation alternative managements for carbon sequestration. Results showed that olive orchards responses in terms of yield and Net Ecosystem Productivity (NEP) vary along with climatic conditions. Water supply was the main driver with a production function that varies for different atmospheric demands. Application of deficit irrigation proved to boost water use efficiency. Besides, intensification from LD to SHD, presented the greatest improvements, 28–73% for yield and 50–100% for NEP. The C sequestration potential of olive orchards was confirmed. In fact, soil organic carbon (SOC) increased continuously over 400 years of simulation, reaching a state of equilibrium. Moreover, intensification and irrigation improved total carbon sequestration. Management of incorporating pruning residues in the soil increased SOC of 10.5 t C ha−1 for Tuscany and 10.8 t C ha−1 for Jaen. Findings of this research enabled the identification of the main drivers influencing the productive and environmental performance of olive groves in the different Mediterranean sub-climates. Impacts of management innovations on olive farming sustainability were also quantified which may help improve production systems for a more sustainable olive cultivation

Chemical equilibration of strangeness

Thermal models are very useful in the understanding of particle production in general and especially in the case of strangeness. We summarize the assumptions which go into a thermal model calculation and which differ in the application of various groups. We compare the different results to each other. Using our own calculation we discuss the validity of the thermal model and the amount of strangeness equilibration at CERN-SPS energies. Finally the implications of the thermal analysis on the reaction dynamics are discussed.Comment: 23 pages, LaTeX (figures included); Talk given at the Int. Symposium on Strangeness in Quark Matter 1997, Santorini (Greece), April 199

Measurement of the Proton's Neutral Weak Magnetic Form Factor

We report the first measurement of the parity-violating asymmetry in elastic electron scattering from the proton. The asymmetry depends on the neutral weak magnetic form factor of the proton which contains new information on the contribution of strange quark-antiquark pairs to the magnetic moment of the proton. We obtain the value $G_M^Z= 0.34 \pm 0.09 \pm 0.04 \pm 0.05$ n.m. at $Q^2=0.1$ (GeV/c)${}^2$.Comment: 4 pages TEX, text available at http://www.krl.caltech.edu/preprints/OAP.htm

Identification of High p⊥\rm p_{\perp} Particles with the STAR-RICH Detector

The STAR-RICH detector extends the particle identification capapbilities of the STAR experiment for charged hadrons at mid-rapidity. This detector represents the first use of a proximity-focusing CsI-based RICH detector in a collider experiment. It provides identification of pions and kaons up to 3 GeV/c and protons up to 5 GeV/c. The characteristics and performance of the device in the inaugural RHIC run are described.Comment: 6 pages, 6 figure

Interventions for preventing or controlling health care–associated infection among health care workers or patients within primary care facilities: A scoping review

Background This review aimed to synthesize the evidence on infection prevention and control interventions for the prevention of health care–associated infection among health care workers or patients within primary care facilities. Methods PubMed, CINAHL, EMBASE, and CENTRAL databases were searched for quantitative studies published between 2011 and 2022. Study selection, data extraction, and quality assessment using Cochrane and Joanna Briggs tools, were conducted by independent review with additional sensitivity checking performed on study selection. Results Four studies were included. A randomized trial and a cross-sectional survey, respectively, found no statistical difference in laboratory-confirmed influenza in health care workers wearing N95 versus medical masks (P = .18) and a significant inverse association between the implementation of tuberculosis control measures and tuberculosis incidence (P = .02). For the prevention of surgical site infections following minor surgery, randomized trials found nonsterile gloves (8.7%; 95% confidence interval, 4.9%-12.6%) to be noninferior to sterile gloves (9.3%; 95% confidence interval, 7.4%-11.1%) and no significant difference between prophylactic antibiotics compared to placebo (P = .064). All studies had a high risk of bias. Conclusions Evidence for infection prevention and control interventions for the prevention of health care–associated infection in primary care is very limited and insufficient to make practice recommendations. Nevertheless, the findings highlight the need for future research

Serum neurofilament dynamics predicts neurodegeneration and clinical progression in presymptomatic Alzheimer's disease

Neurofilament light chain (NfL) is a promising fluid biomarker of disease progression for various cerebral proteopathies. Here we leverage the unique characteristics of the Dominantly Inherited Alzheimer Network and ultrasensitive immunoassay technology to demonstrate that NfL levels in the cerebrospinal fluid (n = 187) and serum (n = 405) are correlated with one another and are elevated at the presymptomatic stages of familial Alzheimer's disease. Longitudinal, within-person analysis of serum NfL dynamics (n = 196) confirmed this elevation and further revealed that the rate of change of serum NfL could discriminate mutation carriers from non-mutation carriers almost a decade earlier than cross-sectional absolute NfL levels (that is, 16.2 versus 6.8 years before the estimated symptom onset). Serum NfL rate of change peaked in participants converting from the presymptomatic to the symptomatic stage and was associated with cortical thinning assessed by magnetic resonance imaging, but less so with amyloid-β deposition or glucose metabolism (assessed by positron emission tomography). Serum NfL was predictive for both the rate of cortical thinning and cognitive changes assessed by the Mini-Mental State Examination and Logical Memory test. Thus, NfL dynamics in serum predict disease progression and brain neurodegeneration at the early presymptomatic stages of familial Alzheimer's disease, which supports its potential utility as a clinically useful biomarker

Amyloid and Tau Pathology Associations With Personality Traits, Neuropsychiatric Symptoms, and Cognitive Lifestyle in the Preclinical Phases of Sporadic and Autosomal Dominant Alzheimer's Disease

Background: Major prevention trials for Alzheimer’s disease (AD) are now focusing on multidomain lifestyle interventions. However, the exact combination of behavioral factors related to AD pathology remains unclear. In 2 cohorts of cognitively unimpaired individuals at risk of AD, we examined which combinations of personality traits, neuropsychiatric symptoms, and cognitive lifestyle (years of education or lifetime cognitive activity) related to the pathological hallmarks of AD, amyloid-β, and tau deposits. Methods: A total of 115 older adults with a parental or multiple-sibling family history of sporadic AD (PREVENT-AD [PRe-symptomatic EValuation of Experimental or Novel Treatments for AD] cohort) underwent amyloid and tau positron emission tomography and answered several questionnaires related to behavioral attributes. Separately, we studied 117 mutation carriers from the DIAN (Dominant Inherited Alzheimer Network) study group cohort with amyloid positron emission tomography and behavioral data. Using partial least squares analysis, we identified latent variables relating amyloid or tau pathology with combinations of personality traits, neuropsychiatric symptoms, and cognitive lifestyle. Results: In PREVENT-AD, lower neuroticism, neuropsychiatric burden, and higher education were associated with less amyloid deposition (p = .014). Lower neuroticism and neuropsychiatric features, along with higher measures of openness and extraversion, were related to less tau deposition (p = .006). In DIAN, lower neuropsychiatric burden and higher education were also associated with less amyloid (p = .005). The combination of these factors accounted for up to 14% of AD pathology. Conclusions: In the preclinical phase of both sporadic and autosomal dominant AD, multiple behavioral features were associated with AD pathology. These results may suggest potential pathways by which multidomain interventions might help delay AD onset or progression