Den site selection and movement patterns of female raccoons following removal and exclusion from residences

Raccoons (Procyon lotor) are one of many wildlife species that have adapted to survive in urban/suburban environments. Classified as a rabies vector species in many eastern states, their disposition after being handled by wildlife specialists is often dictated by this human health concern. Specifically, some states prohibit relocation and mandate that raccoons be released on site or euthanized. Although management using nonlethal means is often preferred by some segments of the human population, several questions remain to be addressed before appropriate agency policies regarding the handling of urban wildlife can be determined. There is little information available regarding the fate of lactating raccoons and their offspring that are trapped and released on site or excluded from human structures. Therefore, our objective was to determine habitat use, home range size, and fate of adult females and their offspring following capture, exclusion, and subsequent release on site. Nineteen adult female raccoons were live-trapped, anesthetized, fitted with radio-collars, and released. Raccoons were captured in Hartford County, Connecticut between April and June of 1998 and 1999. Movements and den-site selection were monitored weekly using radio-telemetry equipment. Home ranges averaged 10.5 ha. Sixty-two percent of the raccoons selected human occupied structures for den sites immediately after release. In total, 73% of the den sites selected were human built. Further insight into nuisance raccoon behavior will permit state wildlife agencies to better develop management policies

Administering GonaCon\u3csup\u3eTM\u3c/sup\u3e to White-Tailed Deer Via Hand-Injection Versus Syringe-Dart

Immunocontraceptive vaccines have shown some promise for fertility control of white-tailed deer (Odocoileus virginianus) in urban and suburban habitats where traditional methods of population control may not be applicable. Currently, the only contraceptive vaccine approved by the U.S. Environmental Protection Agency for use in white-tailed deer is GonaConTM Immunocontraceptive Vaccine, but it is registered for use via hand-injection only. It has been suggested that remote-delivery of immunocontraceptives would be more cost-effective than hand-injection, but there is the potential for incomplete injection from a syringe-dart. Therefore, the purpose of our research was to: (1) conduct a dart configuration assessment trial to determine the ideal syringe-dart configuration for remote-delivery of GonaCon to white-tailed deer and (2) use the determined syringe-dart configuration in a subsequent trial to evaluate the vaccine efficacy when administered to female white-tailed deer via hand-injection versus syringe-dart. We saw comparable results with regard to vaccine dispersal during the dart configuration assessment and the efficacy trial; syringedart injected deer presented vaccine deposits and reaction sites both subcutaneously and intramuscularly, whereas, hand-injected deer presented vaccine deposits and reaction sites only intramuscularly. One year after administration, 4 of 5 deer treated with syringe-darts were pregnant, compared to 3 of 6 deer that received hand-injections. Anti-GnRH titers were negatively related to pregnancy status. We did not observe a high level of vaccine efficacy with the syringe-dart delivery method we used. Therefore, we recommend further research of syringe-dart delivery of GonaCon with a larger sample size where the vaccine is deployed in a single bolus similar to a hand-injected presentation

Managing White-tailed Deer in Suburban Environments

A print on demand of these books and articles can be obtained from Cornell Business Services (CBS) Digital Services by sending e-mail to [email protected] or calling 607.255.2524. In the body of the message include the identifier.uri for the book or article, and ask to be contacted regarding payment.Deer populations in suburban environments are soaring, resulting in an increase in deer-related conflicts such as property damage, vehicle collisions, and altered forest ecology. This publication describes strategies and methods for controlling deer populations in suburban environments and provides extensive resource contacts and a list of state wildlife agencies.Cornell Cooperative Extension The Wildlife Society Northeast Wildlife Damage Management Research and Outreach Cooperativ

Ecology of Argali in Ikh Nartiin Chuluu, Dornogobi Aymag

Argali sheep (Ovis ammon) are listed as threatened in both Mongolia and internationally. Yet, little is known about the biology and ecology of this species. Available data suggests that Argali in Mongolia are declining due to direct poaching and competition with domestic livestock. We initiated several research projects to better understand and conserve the species. In this report we discuss Argali ecology using radio telemetry. We captured and radio-collared 36 Argali using drive-nets, lamb captures, and dating from 2000–2004. Fifteen collared animals have died: 2 due to capture techniques, 8 from predation, 1 from starvation and exposure, 1 from disease, 1 due to maternal neglect, and 2 of unknown causes. In addition, 1 collar ceased working and 4 others dropped off prematurely. We have collected more than 1,040 locations through mid-May 2004. The majority of the Argali were captured in the northern portion of Ikh Nart. Animals have primarily restricted their movements to that area and have not exhibited seasonal movement patterns. Mean home range size for 17 animals with sufficient data (\u3e 45 days with locations) was 57±3.7 km2 (range = 30– 80 km2) using the 100% minimum convex polygon method, with areas of predicted occurrence of 76±5.3km2 for 95% kernel, 32±3.7 km2 for 75% kernel, 11±1.6 km2 for 50% kernel, and 3.8±0.5 km2 for 25% home ranges. Predation was the main cause (72.7%) of mortality in the collared animals for which cause of death could be determined (non-study related)

Low-temperature far-infrared ellipsometry of convergent beam

Development of an ellipsometry to the case of a coherent far infrared irradiation, low temperatures and small samples is described, including a decision of the direct and inverse problems of the convergent beam ellipsometry for an arbitrary wavelength, measurement technique and a compensating orientation of cryostat windows. Experimental results are presented: for a gold film and UBe13 single crystal at room temperature (lambda=119 um), temperature dependencies of the complex dielectric function of SrTiO3 (lambda=119, 84 and 28 um) and of YBa2Cu3O7-delta ceramic (lambda=119 um).Comment: 14 pages, 6 figure

Cystic fibrosis-related diabetes is caused by islet loss and inflammation

Cystic fibrosis-related (CF-related) diabetes (CFRD) is an increasingly common and devastating comorbidity of CF, affecting approximately 35% of adults with CF. However, the underlying causes of CFRD are unclear. Here, we examined cystic fibrosis transmembrane conductance regulator (CFTR) islet expression and whether the CFTR participates in islet endocrine cell function using murine models of beta cell CFTR deletion and normal and CF human pancreas and islets. Specific deletion of CFTR from murine beta cells did not affect beta cell function. In human islets, CFTR mRNA was minimally expressed, and CFTR protein and electrical activity were not detected. Isolated CF/CFRD islets demonstrated appropriate insulin and glucagon secretion, with few changes in key islet-regulatory transcripts. Furthermore, approximately 65% of beta cell area was lost in CF donors, compounded by pancreatic remodeling and immune infiltration of the islet. These results indicate that CFRD is caused by beta cell loss and intraislet inflammation in the setting of a complex pleiotropic disease and not by intrinsic islet dysfunction from CFTR mutation

Spin Foams and Noncommutative Geometry

We extend the formalism of embedded spin networks and spin foams to include topological data that encode the underlying three-manifold or four-manifold as a branched cover. These data are expressed as monodromies, in a way similar to the encoding of the gravitational field via holonomies. We then describe convolution algebras of spin networks and spin foams, based on the different ways in which the same topology can be realized as a branched covering via covering moves, and on possible composition operations on spin foams. We illustrate the case of the groupoid algebra of the equivalence relation determined by covering moves and a 2-semigroupoid algebra arising from a 2-category of spin foams with composition operations corresponding to a fibered product of the branched coverings and the gluing of cobordisms. The spin foam amplitudes then give rise to dynamical flows on these algebras, and the existence of low temperature equilibrium states of Gibbs form is related to questions on the existence of topological invariants of embedded graphs and embedded two-complexes with given properties. We end by sketching a possible approach to combining the spin network and spin foam formalism with matter within the framework of spectral triples in noncommutative geometry.Comment: 48 pages LaTeX, 30 PDF figure

NRF2-driven miR-125B1 and miR-29B1 transcriptional regulation controls a novel anti-apoptotic miRNA regulatory network for AML survival

Transcription factor NRF2 is an important regulator of oxidative stress. It is involved in cancer progression, and has abnormal constitutive expression in acute myeloid leukaemia (AML). Posttranscriptional regulation by microRNAs (miRNAs) can affect the malignant phenotype of AML cells. In this study, we identified and characterised NRF2-regulated miRNAs in AML. An miRNA array identified miRNA expression level changes in response to NRF2 knockdown in AML cells. Further analysis of miRNAs concomitantly regulated by knockdown of the NRF2 inhibitor KEAP1 revealed the major candidate NRF2-mediated miRNAs in AML. We identified miR-125B to be upregulated and miR-29B to be downregulated by NRF2 in AML. Subsequent bioinformatic analysis identified putative NRF2 binding sites upstream of the miR-125B1 coding region and downstream of the mir-29B1 coding region. Chromatin immunoprecipitation analyses showed that NRF2 binds to these antioxidant response elements (AREs) located in the 5′ untranslated regions of miR-125B and miR-29B. Finally, primary AML samples transfected with anti-miR-125B antagomiR or miR-29B mimic showed increased cell death responsiveness either alone or co-treated with standard AML chemotherapy. In summary, we find that NRF2 regulation of miR-125B and miR-29B acts to promote leukaemic cell survival, and their manipulation enhances AML responsiveness towards cytotoxic chemotherapeutics

Exploiting inflammation for therapeutic gain in pancreatic cancer

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy associated with <5% 5-year survival, in which standard chemotherapeutics have limited benefit. The disease is associated with significant intra- and peritumoral inflammation and failure of protective immunosurveillance. Indeed, inflammatory signals are implicated in both tumour initiation and tumour progression. The major pathways regulating PDAC-associated inflammation are now being explored. Activation of leukocytes, and upregulation of cytokine and chemokine signalling pathways, both have been shown to modulate PDAC progression. Therefore, targeting inflammatory pathways may be of benefit as part of a multi-target approach to PDAC therapy. This review explores the pathways known to modulate inflammation at different stages of tumour development, drawing conclusions on their potential as therapeutic targets in PDAC

One-carbon metabolism in cancer

Cells require one-carbon units for nucleotide synthesis, methylation and reductive metabolism, and these pathways support the high proliferative rate of cancer cells. As such, anti-folates, drugs that target one-carbon metabolism, have long been used in the treatment of cancer. Amino acids, such as serine are a major one-carbon source, and cancer cells are particularly susceptible to deprivation of one-carbon units by serine restriction or inhibition of de novo serine synthesis. Recent work has also begun to decipher the specific pathways and sub-cellular compartments that are important for one-carbon metabolism in cancer cells. In this review we summarise the historical understanding of one-carbon metabolism in cancer, describe the recent findings regarding the generation and usage of one-carbon units and explore possible future therapeutics that could exploit the dependency of cancer cells on one-carbon metabolism